查询词典 sequencing problem

Long-PCR amplification, clone and primer-walking sequencing methods were employed in determine the complete sequence of mitochondrial genome of tokay.

采用长PCR扩增、克隆和引物步行等方法，测定了大壁虎线粒体基因组全序列。

This year, on the 150th anniersary of the discoery of the Neandertal type specimen, researchers in Europe and the UnitedStates transformed the study of this ancient human by sequencing more than 1 million bases of Neandertal DNA.

今年是发现穴居人标本150周年，研究者在欧洲和美国开始通过超过1百万碱基对的穴居人DNA测序，来研究此种古人类。

The direct sequencing of uncultivable organisms present in complex biological and environmental samples has opportunities to discover new life forms and metabolic processes.

在复杂生物和环境样本中出现的不可培养的生物的直接测序具有发现新的生命形式和代谢过程的机会。

George Church, a molecular geneticist at Harvard Medical School and a pioneer of whole-genome sequencing, is unpersuaded.

哈佛医学院的分子遗传学家George Church，同时也是人类基因组测序的先驱则不为所动。

Morphological and physiological identification showed that NTG-01 was Gram-negative, short rod shape, with flagella around the bacteria, V-P test positive and Methyl red test negative. The bacterium can use glucose as carbon suource and produce gases. No H2S produced on TTS medium. Lysine decarboxylase and orthinine decarboxylase, catalase and gelatine test are positive. Arginine decarboxylase, dehydrolase, oxidase and urase test are negative. Sequencing and alignment of 16S rDNA showed that it shared a 99% homologous sequence with Enterobacter aerogenes.

结果显示菌株NTG-01为细菌，革兰氏染色阴性，短杆状，鞭毛周生，V-P试验阳性，甲基红实验阴性，利用葡萄糖产酸产气，不能在TIS培养基上产生H_2S，赖氨酸和鸟氨酸脱羧酶试验呈阳性，精氨酸脱羧酶和双水解酶试验呈阴性，氧化酶和脲酶试验阴性，接触酶和明胶液化试验阳性，但后者反应迟缓，一周后才观测到结果。

In addition, it is very difficult to perform sequencing at levels of viremia below 200 IU/ml.

而且，对于病毒载量低于200 IU/ml的样品，测序往往也很难进行。

Sequencing and homology analysis revealed that the gene fragment was 395 bp in length and shared up to 89% similarity with NB gene from winter squash. The deduced amino acid sequence for the fragment was composed of 131 amino acid residues.

经过测序和同源性分析表明：该基因片段长度为395bp，与GenBank上所登录的笋瓜NR基因同源性达89%，根据所测序列推导该片段由131个氨基酸残基组成。

The principle and foundation of selecting and sequencing the important defending targets in defense zone are analyzed.

分析了防空重点保卫目标优选的依据和原则，构建了评价保卫目标重要程度的指标体系。

Seven major bacterial isolates were obtained and purified. Their 16S rRNA genes were amplified by PCR for sequencing and their identities were determined with homology comparisons. Five of the seven isolates belong to Actinomycetales including Kocuria KD139, Rhodococcus KD140, Rhodococcus KD142, Arthrobacter KD230, and Arthrobacter KD232; one is classified as Bacillus d KD178; and another one as Stenotrophomonas KD237. The phylogenetic tree was also constructed based on the analysis of 16S rRNA gene sequences.

通过对分离菌株的16S rRNA基因序列进行分析，发现其中5株细菌分别属于放线菌目的考克氏菌属(KD139)、红球菌属(KD140和KD142)和节杆菌属(KD230和KD232)， 1株细菌属于杆菌目的芽胞杆菌d属(KD178)，另外1株细菌属于黄色单孢菌目的寡食单胞菌属(KD237)；同时我们构建了系统进化树，确定分离菌株的相对进化地位。

Results The activity of FⅫ were 52.5%, 78.6% and 89% for the proband, his father and mother, respectively. Direct sequencing suggested that the proband was 46 T/T genotype in exon1, while both his parents were 46 C/T genotype in the same alle.

结果 先证者及其父、母FⅫ：C分别是52.5%、78.6%、89%，直接测序结果提示先证者F12基因的第1外显子46位碱基即启动子上游-4位为T/T基因型，其父、母在该位点均为C/T基因型。

Don not attempt to do something which you can not to do.

不要企图做那些办不到的事情。

The expression of CTGF and TNF-αweredetected by immunochemistry and the number of Clara Cells was calculated.

光镜下观察肺组织的病理变化，采用免疫组化染色观察肺组织中结缔组织生长因子和肿瘤坏死因子-α的表达和Clara细胞的数量。