查询词典 sequenced

The full-length DNA-A molecules from ageratum sample G8 and G10 were cloned and sequenced. Sequence analyses and comparisons showed DNA-A of G8 and G10 shared 93.2% identity to each other, So G8 and G10 were isolates of a same begomovirus.

从胜红蓟样本G8和G10中克隆并测定了两个DNA-A全序列，序列比较分析发现，G8和G10之间的同源性达到93.2％，认为是同一病毒的不同分离物。

During the postgenomics era the information about genomic sequence and gene functions provides a new foundation for evolutionary biology and ecology As the first whole-genome sequenced plant Arabidopsis thaliana and its wild relatives have played a critical role in understanding the evolution of genomics and speciation Both A halleri and A lyrata are closely related to the model species A thaliana A halleri ssp gemmifera occurs in northeastern China Japan and Taiwan; while its sister A halleri ssp halleri is mainly distributed in Europe Geographical barriers such as Tienshan Mountain Range isolate these intraspecific sisters Likewise A lyrata ssp kamchatica and ssp lyrata occur in East Asia and North America respectively Such distribution patterns seem to be consistent with allopartic speciation The comparison between ancestral and extant polymorphism by multilocus can be informative about the population genetics of speciation In this study we collected and analyzed DNA sequences of 98 genes from four wild relatives of A thaliana A halleri ssp gemmifera A halleri ssp halleri A lyrata ssp kamchatica and A lyrata ssp lyrata The ancestral states of these four species were compared to each other in terms of level of genetic variation However the ancestral species at the time of speciation were substantially more polymorphic than the extant geographical populations The observations are not fully compatible with speciation by strict allopatry At some species pairs parapatric speciation seems more reasonable in speciation of Arabidopsis The 98 gene sequences are also used for the congruence test between gene genealogy and species phylogeny Only 28 genes support the species phylogeny but there are 23 genes supports another major genealogy { lyrata} thaliana Based on the phylogenetic position change of A lyrata ssp kamchatica and Ks value for each species pair suggested the recent directional gene flow between A halleri ssp gemmifera and A lyrata ssp kamchatica

阿拉伯芥是第一个完成基因体定序的开花植物，其基因体资讯提供植物学研究的重要依据；在解析阿拉伯芥属物种的亲缘关系以及种化机制等重要的演化议题时，阿拉伯芥近缘的野生物种自然成了不可或缺的关键；跟阿拉伯芥近缘的物种包括A halleri及A lyrata，其中A halleri ssp gemmifera主要分布於中国东北、日本以及台湾，与近缘的A halleri ssp halleri其分布於欧洲隔著天山及大陆的障蔽，而A lyrata ssp kamchatica主要分布於东北亚及台湾，与分布於北美五大湖的A lyrata ssp lyrata被北极圈所分隔，这样的分布模式暗示异域种化的可能。藉由多基因分析比较祖先物种与现生物种遗传歧异度的相关可提供讯息探讨种化时期的族群遗传结构，本研究针对A halleri ssp gemmifera、A halleri ssp halleri及A lyrata ssp kamchatica、A lyrata ssp lyrata四个物种，两对互为亚种的姊妹群，以阿拉伯芥为外群进行研究，在四个物种完成98个同源基因的分子序列，利用套装软体MCMCcoal来估算祖先物种的遗传变异，亦估算现生物种的核苷酸歧异度，观察到？多物种配对中祖先物种遗传多型性大於现生物种DNA歧异度，显示异域种化模型并无法完全解释阿拉伯芥属物种的种化模式，在某些物种配对间邻域种化模式应比异域种化更为可能；在基因树与物种树的比较，98个基因片段的亲缘模式只有28个是与已知物种树一致的，有23个基因其树状图支持{ lyrata} thaliana的型式，藉由kamchatica位置的变化以及估算各物种配对间的平均同义置换率，推测在A halleri ssp gemmifera与A lyrata ssp kamchatica间具有近代的单方向基因交流。

Abstract:Seven species of Amanita, collected in Lishui of Zhejiang Province, China, were identified primarily based on morphological characteristics. The rDNA ITS regions of these species were cloned and sequenced, and the characteristics of ITS sequences were compared and analysed.

常规对像鹅膏菌属大型真菌的分类鉴定主要是依据子实体的形态，颜色，气味，以及孢子特征等，这些形态特征经常是易变的，模糊的，易混淆的，因而依据传统的形态特征进行分类鉴定总是带有一定的局限性和主观性，要准确鉴定鹅膏属真菌到某一个种实属困难。

The light and heavy chain variable region gene of the monoclonal antibody had been cloned and sequenced. VH region gene consisted of 350 bp, and VL region gene consisted of 321 bp encoding 107 amine acid residues .

采用分子生物学方法，对抗RB单克隆抗体的轻重链可变区基因进行克隆及序列分析，重链约350bp，轻链为321bp，编码107氨基酸。

MethodsA phage random twelve peptide library was screened 4 times by monoclonal antihuman FGF7. ELISA staining examination was performed to choose monoclonal phages at random. Also, DNA extracted from phages with better bindingactivity was sequenced and genecompared.

方法以hFGF7单克隆抗体为靶，进行4轮生物淘选噬菌体随机十二肽库；随机挑取单克隆噬菌体，应用ELISA方法检测单克隆噬菌体的结合力，对结合力较好的单克隆噬菌体提取噬菌体DNA，进行测序，并行序列分析。

You can create an application package, also called a sequenced application, by using the Application Virtualization Sequencer.

您可以创建一个应用程序包，也称为顺序应用程序通过使用应用程序虚拟化序列。

We have sequenced and annotated the genome of the filamentous ascomycete Ashbya gossypii. With a size of only 9.2 megabases, encoding 4718 protein-coding genes, it is the smallest genome of a free-living eukaryote yet characterized. More than 90% of A.

作者对丝状子囊菌Ashbya gossypii进行测序和注解发现基因组有9.2Mb并具有4718个蛋白编码基因，它是迄今描述的最小的自由生长真核细胞。90％以上的A。

Chromosome 22 is the first sequenced human autosome since Human Genome Project was launched.

22号染色体是人类基因组计划实施以来最先完成序列测定的常染色体。

Methods A nested RT-PCR was used for detecting one fragment of SARS-CoV RNA in oropharyngeal swabs from 3 SARS probable patients ,4 SARS suspect patients and other 27 patients with fever in Hangzhou, and the nested RT-PCR product from one SARS probable patient was sequenced.Meanwhile in these 3 SARS probable patients, other three RT-PCR methods, including a hemi -nested RT-PCR targeted for another fragment of SARS-CoV RNA, a real-time RT -PCR and a modified nested real-time RT-PCR, were employed to detect SARS-Co V RNA.Results Two positives were found in the 3 SARS pro bable patients, and none positive in 4 SARS suspect patients and other 27 patien ts with fever, using the nested RT-PCR.

方法对2003年杭州市3例严重急性呼吸综合征临床确诊患者、4例疑似和27名医学观察者的咽拭子标本用巢式实时RT-PCR检测SARS-CoV核酸，对阳性扩增产物进行核酸序列测定，同时对3例患者的标本应用半巢式RT-PCR检测另一位点SARS-CoV核酸片段，并应用常规实时RT-PCR技术及改良实时RT-PCR技术进行检测。

Ravel also sequenced the genome of a Bacillus subtilis strain that was found in one of the letters.

瑞福对于在其中一封信中发现的枯草芽孢杆菌也进行了测序。

Chimborazo and Cotopaxi, took me by the hand.

越过琴博腊索山和科托帕克西山。

This car is in a good condition.

这辆车的状况很好。