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Methods Expressions of MMP2 and TGF-β1 gene from 43 human suprarenal epithelioma patients' carcinoma and tumor-adjacent tissues were detected by semi-quantitative reverse transcription polymerase chain reaction.

应用半定量逆转录－聚合酶链反应检测43例肾透明细胞癌患者癌与癌旁组织MMP2和TGF-β1的表达水平。

Methods Semi -quantitative reverse transeriptase - polymerase chain reaction was used to examine the expression of BCRP gene in 62 cases of malignant hematologic diseases, BCRP/β2 - MG ratio was defined as BCRP expressible level.

方法应用半定量逆转录-聚合酶链反应，β2微球蛋白基因作内参照，以BCRP／β2-MG的比值作为BCRP基因的相对表达水平，检测62例恶性血液病BCRP基因的表达。

METHODS: Liver fibrosis models were established in 24 SD rats by intragastric administration (7 g/kg) of alcohol (56°, 560 mL/L) every morning, and 10 normal rats served as controls. The rats in model group were killed by exsanguination from femoral vein at 4, 12 and 24 wk. Pathological changes of liver tissues in rats were identified by Masson staining, and the expression of VEGF mRNA was detected by semiquantitative reverse transcription-polymerase chain reaction.

56度的白酒(560 mL/L)平均以7 g/kg的剂量每日早晨灌胃一次制备肝纤维化模型，灌胃4 wk、12 wk及24 wk采用股静脉放血法分别处死大鼠，观察肝脏病理变化并采用半定量逆转录聚合酶链反应检测VEGF mRNA的表达。

Polymerase Chain Reactionwas performed using P1and P2as primers andthese eyestalk cDNAs as templates.Four specific cDNAs were cloned under properreaction conditions.

用无DNA污染的眼柄总RNA为模板，根据日本对虾的MIH设计兼并引物P1和P2，进行RT-PCR扩增，在适宜的反应条件下，均分别得到一特异性的产物。

The interaction between DNA polymerase and DNA is important to ensure the high fidelity of DNA replication.

DNA聚合酶与DNA的相互作用对于生物体确保DNA复制的正确性具有重要意义。

A constantly fluctuating brigade of enzymatic foot soldiers reg ulates RNA polymerase II's activity by chemically modifying the long cord-li ke tail that hangs from its globular structure, like a chain on a light fixt ure.

有一组不断变动的酶通过化学修饰RNA聚合酶II球状结构长带状尾巴来调节RNA聚合酶II的活性，就像电灯组件后电线。

The study cloned the flic gene of App and performed sequencing and analysis of biological information. Polymerase chain reaction PCR was used to amplify the flic gene from.

构建猪传染性胸膜肺炎放线杆菌(Actionobacillus pleuropneumoniae， App)鞭毛蛋白编码基因的重组表达质粒，对其编码蛋白进行生物信息学分析，并将其在原核细胞中进行表达。

Objective:To develop a real-time florescence quantitative polymerase chain reaction method for quantitation of circulating DNA in plasma and explore its value in the diagnosis and prognosis of breast cancer.

目的：建立一种荧光定量PCR方法用于血浆循环DNA定量，并讨论血浆循环DNA检测在乳腺癌诊断及预后中的意义。

Aim: To study the feasibility of aneuploidy diagnosis using coelocentesis combining with quantitative fluoresce polymerase chain reaction in early pregnancy.

目的：探讨应用荧光定量PCR法检查早孕期胚胎胚外体腔液胎儿细胞染色体的可行性。

Results The serum antibody level reached to 0.71±0.08-0.77±0.06 after genetic vaccine pCD-HCV1(100 μg/mouse) were inoculated into the mice(n=12) three or four times while blank vector pCD-SRα1 could not induce the mice(n=8) to generate antibody response in same way.After the antibody levels in mice(n=8) immunized by pCD-HCV1 had ascended to peak value(0.71), there was no trend of descending during the following 18 weeks of detection(0.68±0.06-0.75±0.07). Specific fragment of HCV cDNA identified by polymerase chain reaction from DNA extracted from the muscles of the mice after pCD-HCV1 had beed inoculated three months.

结果 基因疫苗pCD-HCV1(100 μg/只)3-4次接种小鼠后(n=12)，血清抗体水平达0.71±0.08-0.77±0.06，空载体pCD-SRα1免疫鼠血清抗体阴性；免疫鼠(n=8)抗体水平达高峰后(0.71)，持续检测18周未见下降趋势(0.68±0.06-0.75±0.07)；pCD-HCV1免疫小鼠3个月后，用聚合酶链反应仍可从肌肉组织中扩增出特异性HCV cDNA片段；免疫鼠PBMC对HCV合成肽CP9、基因重组抗原C、E1刺激后均出现增殖反应，刺激指数分别为4.07±1.58、3.88±0.70和3.69±1.30，与pCD-SRα1免疫鼠相比，差异有显著性(P.001)。

He and Nina moved to California and lived at 2005 Ivar Street, Apt.

他和Nina搬到加州，并在2005年伊瓦尔街，公寓生活。

Droperidol （ potently inhibits transfected HERGchannels and this is the probable mechanism for QT prolongation.

氟哌利多有效地抑制了转染的 HERG 钾通道，可能是 QT 间期延长的机制。