查询词典 polymerase

A pair of primers were designed based on M1 gene sequence of known H5N1 Avian influenza virus.M1 gene was cloned from total RNA,extracted from tissue of H5N1 subtype virus inoculated embryo by reverse transcriptase-polymerase chain reaction using high proofreading polymerase(Pyobest~TM DNA Polymerase),and expressed using Invitrogen champion~TM pET directional TOPO expression system.Recombinant protein containing polyhistidine(6xHis) tag in N-terminal about 29.8kDa in size,wac obtained and purified.

根据已发表的禽流感病毒M1基因序列设计合成PCR克隆引物，自接种H5N1亚型病毒的鸡胚组织中提取RNA，反转录后采用高可信度DNA聚合酶经PCR扩增M1基因，采用Invitrogen定向表达系统(ChampionTMpET directional TOPO expression system)进行克隆表达，纯化获得N末端携带多聚组氨酸标签的重组蛋白，分子量约29.8 kDa。

Furthermore, by using two-colored probes to study the relationships between RNA polymerase Ⅲ and RNA polymerase I, we observed that the signals of the two RNA polymerase were very close.

同时，应用这一研究方法对RNA聚合酶Ⅰ的转录发生在核仁中的结论进行了确证，并进而应用双探针标记荧光原位杂交的方法对RNA聚合酶Ⅲ和RNA聚合酶Ⅰ转录位点的关系进行了初步探讨，结果显示两者的转录位点之间非常接近，并且有伴随发生的趋势。

Based on the fact that the dye probe can intercalate DNA duplex to emit fluorescence, a novel method for detection of DNA polymerase has been developed. The polymerization process has been indicated by fluorescent signal in real-time. Based on this assay, the influence of anti-tumour drugs on the activity of polymerase has been investigated.

利用染料探针技术，建立了一种简便快捷地实时监测聚合酶活性的方法，探讨了三种抗肿瘤药物对聚合酶活性的影响，为以聚合酶为作用靶标的抗肿瘤新药的开发及筛选提供了一种新的思路。

The results of these early research work showed that RNA polymerase Ⅰ transcription was localized in the nucleoli and RNA polymerase Ⅱ and Ⅲ in the nucleoplasm.

当时的研究结果显示：RNA聚合酶Ⅰ的转录发生在核仁内，RNA聚合酶Ⅱ和RNA聚合酶Ⅲ的位于核质中。

The results of these early research works showed that the localization of RNA polymerase I transcription was in the nucleoli and that of RNA polymerase II and Ⅲ in the nucleoplasm.

研究结果显示：RNA聚合酶Ⅰ的转录发生在核仁内，RNA聚合酶Ⅱ和RNA聚合酶Ⅲ的转录发生在核质中。

The transcription localizations of these polymerases have been studied since early 1960s. The results of these early research works showed that the transcription of RNA polymerase I was localized in the nucleoli while that of RNA polymerase Ⅲ in the nucleoplasm.

自上个世纪六十年代初期，人们相继运用细胞化学染色、电镜放射自显影等进行研究的结果表明：RNA聚合酶Ⅰ的转录发生在核仁中，RNA聚合酶Ⅲ的转录发生在核质中。

To compensate the descent of pH for the heat-up, exalting the Tris-HCl buffer's pH can avoid the abscission of the purine and make the long sequence PCR go on smoothly. The GC-rich template's secondary structure is complex and stable. The organic reagent methylamine contributes to uncoil the secondary structure.The pfu DNA polymerase has more fidelity and thermostability than Taq DNA polymerase.

高GC含量使模板二级结构复杂而稳定，一般热启动难于解开，而变性温度过高、时间过长又不利于DNA聚合酶的活性作用发挥，于是借助能缓释模板二级结构的有机试剂甲胺扩增高GC含量模板。pfu DNA聚合酶比Taq酶有更高的保真复制性能和热稳定性，更有利于高GC含量模板的扩增。

The recombi- nant hepatitis B virus polymerase expressed in cells infected by recombinant baculovirus was determined by SDS-PAGE, The hepatitis B virus polymerase was expressed in insect cells at high level.

结论利用杆状病毒表达系统，构建的重组杆状病毒在昆虫细胞中高效表达乙型肝炎病毒多聚酶，为进一步的乙型肝炎病毒多聚酶的体外功能研究奠定了基础。

However, with the development and the application of new techniques, the reliability of the methods used in the research of the transcription site of RNA polymerase I was doubted; and also, some indirect results indicated that the transcription of RNA polymerase Ⅲ might occur in the nucleoli.

但随着新的研究技术的发展和应用，人们对RNA聚合酶Ⅰ转录位点的早期的研究方法的可靠性提出质疑；同时，根据一系列间接的实验推论RIgA聚合酶Ⅲ的转录可能发生在核仁中。

NodD binds to and bends target promoters through anchoring two tandem and individual specific DNA sites. NodD functions as a tetramer, which has a V-shaped main body. Tetrameric NodD is to change its own conformation rather than its oligomeric forms in response to small signal molecules. The specific interaction between each NodD DNA-binding domain and each specific DNA site does not alter itself in spite of naringenin induction, and the induced conformational change is transferred from protein to DNA. Only the DNA conformation incited by induced NodD is competent for RNA polymerase to form the transcriptional open complex. It cannot be excluded that NodD may have protein-to-protein contacts with RNA polymerase, and that the NodD conformational change may also directly contribute to the transcriptional open complex formation. However, the NodD conformational change itself cannot serve as the determinant of the transcriptional molecular switch.

通过研究，我们提出了初步的NodD操纵子激活模型：第一，四聚体是NodD蛋白的功能单位，它通过铆钉两个串联的相对独立的DNA靶位点结合被诱导的启动子；第二，小分子配基的结合是改变NodD四聚体的构象而不是引发不同形式的寡聚体，在我们的模型中，NodD四聚体缩小其V形主体的弯折角，进而缩短其DNA结合功能域的间距；第三，小分子信号的诱导并没有改变NodD的DNA结合域和其DNA靶位点的相互作用，NodD的构象改变由蛋白质经其双铆钉位点传递给DNA；第四，只有诱导状态的NodD激发的DNA构象才能有效地使RNA聚合酶形成转录开放复合物；第五，不排除NodD与RNA聚合酶可能有直接的相互接触位点，不排除NodD构象的改变可能直接有利于RNA聚合酶形成转录开放复合物，但是我们认为NodD构象改变本身不是充当转录激活开关的决定因素。

I can not negligent, he should do what?

我不能不在意了，他要做什么呢？

It is very dangerous to swim in the river alone.

一个人到河里游泳是很危险的。