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As they are evenly dispersed throughout genomes, usually characterized as codominant and highly polymorphic systems amenable to genotyping by polymerase chain reaction, and generally inherited in a Mendelian fashion, microsatellites have become instrumental as genetic markers in many areas.

摘 要：微卫星标记广泛分布于原核生物和真核生物基因组中，具有密度大、多态性丰富、遵循孟德尔分离定律共显性遗传、易于PCR扩增等特点，已成为近年来最引人注目的新型DNA标记。

The method of detecting coliform using Polymerase Chain Reaction is established.

建立了饮用水大肠杆菌的聚合酶链式反应检测方法。

Target DNA fragments were amplified by polymerase chain reaction from the liver tissue of Columba livia using a pair of specific primers, and the amplified PCR products were cloned into pMD18-T vector.

利用特异引物，通过聚合酶链式反应(polymerase chain reaction， PCR)技术，从家鸽肝脏组织的总DNA中扩增到目的片段，并将扩增产物克隆到pMD18-T载体中，经菌落PCR与酶切鉴定、序列测定及序列分析。

In this thesis, we studied the effects of nuclear actin on gene transcription regulation, with a special focus on the relations between the nuclear actin and RNA polymerase II in concanavalin A-stimulated lymphocytes and HeLa cells.

本文以ConA激活的淋巴细胞和HeLa细胞为实验材料，从不同角度研究了细胞核肌动蛋白与RNA聚合酶Ⅱ相互作用，并对细胞核肌动蛋白参与RNA聚合酶Ⅱ的作用机制进行了探讨。

Objective To establish a method for rapid detection of clinical suspect fungal corneal ulcer by polymerase chain reaction.

目的 探讨聚合酶链反应快速检测在真菌性角膜溃疡诊断中的价值。

Methods The mutations in blood corpuscle mitochondrial DNA T2DM without consanguinity and controls were determined by polymerase chain reaction-restriction fragment length polymorphism.

采用聚合酶链反应-限制性片段长度多态性法对无血缘关系的糖尿病患者及正常对照个体的血细胞线粒体DNA进行突变分析。

After 240 weeks, the cumulative probability of HBV polymerase mutations was 29%, but the cumulative probability of mutations with virologic resistance was 20% and of mutations, virologic resistance, and ALT elevations was 11%.

在治疗240周后，HBV聚合酶突变、病毒学抵抗和临床抵抗累积率分别为29%、20%和11%。4例患者有轻度肌酐水平升高。

Methods The deletion and mutations of exon5, 8 of FHIT gene were detected in 10 cutis primary T cell lymphoma samples tissues by polymerase chain reaction and single-strand conformation polymorphism methods.

用PCR-SS-CP法检测了10例原发性皮肤T细胞淋巴瘤患者皮损FHIT基因外显子5和8的缺失和突变状况，10例健康皮肤组织做对照。

A full-length cDNA of cytolysin was obtained by reverse transcriptase-polymerase chainreaction, using primers designed according to the EST sequence from the cDNA library.

玫瑰红绿海葵触手cDNA表达文库的成功构建，为研究海葵触手毒腺的活性组分和分子作用机制奠定了基础，也是分离新基因的重要资源。

Objective To establish a method for quantitative detection of cytotoxin associated gene A positive Helicobacter pylori by competitive polymerase chain reaction.

目的 建立竞争性聚合酶链反应定量检测幽门螺杆菌细胞毒素相关蛋白基因A的方法。

This paper designs the communication system of radio telescope FAST.

针对500m口径球面射电望远镜FAST分布式控制系统的特点完成了其通信系统的设计。

Political sheep's step-by-step a large number of fossils unearthed in the county soil Ma Ali after rural village in the middle of sallow pelitic siltstone.

步氏和政羊化石大量出土于广河县阿力麻土乡后山村，产于灰黄色泥质粉砂岩中。