查询词典 modifier gene

The cry8C gene from Bt strain HBF-1 was cloned and sequenced. There was one amino acid difference with cry8Cal gene from Bt strain Buibui and it was designed as cry8Ca2 gene (AY518201) by International Bt Insecticidal Protein Gene Nomenclatural Committee.

从菌株HBF-1中克隆的cry8C基因与cry8Cal基因有一个氨基酸的差异，被国际Bt杀虫晶体蛋白基因命名委员会命名为cry8Ca2基因。

In order to understand the function of this gene within short time, we also constructed yeast (Schizosaccharomyces pombe vector to analyze the gene function, but the result investigated that over expression of this gene could not increase the length of yeast cell. It suggested that expression of the gene is not a direct reason in cell elongation.

为了在短时间内初步研究该基因的功能，构建了酵母表达载体，利用裂殖酵母表达系统对该基因的功能进行活体分析，没有发现该基因对单核的酵母细胞的伸长有明显影响。

All the LOH on 〓 and 〓 was observed in invasive ductal carcino- ma, carcinoma simplex, medulary carcinoma and scirrhous carcino- ma, no deletions at these sites were observed in any invasive lobular carcinoma and others. These results imply an etiological difference.P53 gene is a hot point gene in the occurrence and development of breast, cancer. PCR-SSCP analysis was performed to detect P53 gene point mutation in the region between exon 5 and 8, 5 of 12 (41. 6%) stage I breast cancer patients contain mutation of P53, 3 of 5 patients were accompanied by 〓 deletion. These results suggested that point mutation and allelic loss of P53 gene are two vi- tal genetic events in earlier stage of breast tumorigenesis.

我们还发现，〓和〓位点LOH均分布在乳腺浸润性导管癌、单纯癌、髓样癌及硬癌中，在浸润性小叶癌和某些特殊类型乳腺癌中全部为LOH阴性，上述位点LOH可能与某些组织学类型乳腺癌的发生有关。P53基因是乳腺癌形成过程中的一个热点基因，本研究对12例Ⅰ期乳腺癌组织标本中P53基因热点区域第5、6、7、8外显子点突变进行了测定，发现41.6％（5／12）的病人有P53基因一个或多个点突变，其中3例同时伴有〓位点LOH，表明P53基因点突变和等位基因缺失是发生在乳腺癌形成早期的一个重要遗传学事件。

Deduced by the proportion of typical sterile plants and normal fertile plants in segregative generations derived from WA zhenqiuA/6078 that:I gene could inhibit the expression of Rf gene completely by the heterozygosity of 1 pair of Rf in genotype;and only reduce the expression of Rf genes with two heterozygous Rf genes in genotype ;but it would never inhibit the expression of Rf genes if the genotype included 3 pairs of heterozygous Rf genes. When Rf gene was homozygous,the / gene could not inhibit the expression of it.

根据认叭真秋刀6078各衍生分离世代中典型不育株和正常可育株所占比例推断：基因型中仅包含1对杂合的Rf基因时，I对Rf的表达起完全抑制作用；基因型中包含2对杂合的Rf基因时，I仅对Rf的表达起削弱作用；当基因型中包含3对杂合的对基因时，I对Rf的表达不起抑制作用；I对纯合位点的Rf的表达不起抑制作用。

In the present paper, the Sox gene expression analysis of different tissues from the Trionyx sinensis was studied by using RTPCR, and Sox gene fragments of expression from the testicle, brain and spleen were cloned using RTPCR products. The results show that Sox gene has specific expression in the testicle, brain, spleen, cardiac muscle and kindney and hasn't expression in muscle, liver and ovary. The results of clone reveal that the Sox genes of expression in the testicle are TSSox1 and TSSox 4, and those are TSSox2 and TSSox4 in the brain, and that is TSSox4 in the spleen. This suggests that the Sox gene act important role not only on the sex determination, but also on the development of neural system, immunocyte system and the differentiattion of male germ cell.

本文采用RT—PCR技术，研究了中华鳖不同组织Sox基因的表达，并通过PCR直接克隆法，分析了来自睾丸、脑和脾组织中的Sox基因序列结果表明在中华鳖的成体组织中，Sox基因在脑、心肌、肾、脾和雄性的睾丸组织中均有不同程度的表达，而在肌肉、肝脏和雌性的卵巢中则无表达，显示该基因具有组织表达特异性克隆分析显示，在睾丸组织中表达的是TSSox1和TSSox4基因，而在脑组织中表达的是TSSox2和TSSox4基因，脾组织中表达的是TSSox4基因此结果表明Sox基因不仅在性别决定中起作用，还可能在神经系统、免疫系统多种组织中起重要作用

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

This is a very specific form of gene therapy that, if successful, will circumvent one the main objections to current gene therapy methods; namely, current methods insert the unmutated copy of a gene randomly into the genome, and if the insertion point happens to be near an oncogene, the gene therapy will cure one disease but cause another.

这种基因疗法是非常非常精确的。如果成功，现行的所有基因疗法将&报废&。现在的基因疗法是将正常的基因备份随机的加入到基因组中。如果切入点不对，疾病仍然可能发生。通过Rosetta研究出来的方法可以避免这一弊端。

Of the 32 articles, 4 were about the selection of destination genes, 3 about the selection of target cells, 7 about the selection of gene expression vector, 3 about the methods of gene transfer, 4 about gene therapy combined with tissue engineering in treating bone defect, 5 about polygenes in treating bone defects, and 2 about the problems and prospects in the gene therapy of bone defect.

符合纳入标准的32篇文献中，4篇涉及目的基因的选择，3篇涉及靶细胞的选择，7篇涉及基因表达载体的选择，3篇涉及基因转移方法，4篇涉及基因治疗与组织工程结合治疗骨缺损，5篇涉及多基因联合治疗骨缺损，2篇涉及骨缺损基因治疗存在的问题与展望。

Four pairs of primes were synthesized to amplify Salmonella genus special genes such as hut gene(495 bp), hilA gene(490 bp), invA gene(284 bp) and hns gene(152 bp) of Salmonella, respectively. The PCR reaction condition was optimized.

近年来，分子生物学方法如聚合酶链式反应在沙门氏菌的检测上已得到较好应用[1-6]，大大缩短了检测时间，但各报道中所用的DNA提取方法、引物、PCR反应条件各异。

It could be useful for the further study of NS1 gene function. Method The NS1 gene of H5N1 subtype AIV was amplified by RT_PCR and cloned to pGEM_T_easy vector. The NS1 gene expressing plasmids was constructed by inserting the target gene fragments into pGEX_4T_1 vectors. The expression proteins were indued by IPTG and analysed by Western blot.

方法采用RT_PCR方法对H5N1亚型AIVNS1基因进行扩增，将PCR产物克隆于pGEM_T_easy载体，将该基因插入pGEX_4T_1中构建NS1基因原核表达载体，转化BL21大肠杆菌后，在IPTG诱导下表达NS1蛋白，Westernblot鉴定表达NS1蛋白。

I want to be a big fish in a small pond.

我要做一条在小池塘里的大鱼。

While the software functioned well, we did not feel the same about their customer support!

虽然该软件运行良好，我们没有同样的感觉他们的客户支持！