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Genetic analysis were carried out to identify powdery mildew and strip rust resistance genes in the F2 of Am6-4 amphiploid and wheat variety Jinan17. Results showed that resistances to powdery mildew and stripe rust were controlled by a single dominant gene respectively. 124 SSR primers from genome D were used for marker analysis, marker Xgwm98 150(150为下标) from the chromosome 6D was found to be linked to the new powdery mildew resistance gene with a linkage distance 20.42 cM; A special DNA band was amplified by primere xgwm33 in resistant stripe rust plants, resistance gene for stripe rust was localized on chromosome 1D, and the genetic distance between resistance gene and marker is 8.0 cM.

利用Am6-4与济南17F2分离群体进行白粉病和条锈病抗性基因的遗传分析结果证明,Am6-4中的抗白粉病和抗条锈病基因均为单显性基因;以124对D基因组SSR引物进行标记分析,引物Xgwm98在抗白粉病DNA池和单株中能扩增出特异标记带,标记与抗白粉病基因间的遗传距离为20.42cM,并将抗白粉病基因定位于6D染色体;引物Xgwm33能在抗条锈病DNA池和单株中扩增出特异标记带,标记与抗条锈病基因间的遗传距离为8.0cM,并将抗条锈病基因定位于1D染色体。

The system gets two image of one object with two CCD, thus obtains depth from two CCD"s parallax. After investigating the operation spot and discussing with surgeons we use LED as markers, and get the marker"s 3-D position with a non-contact optics orientation method. After calibrating the CCD, processing the image, the system can figure out marker"s areal coordinates, get marker"s 3-D coordinate in dummy world coordinate system, thereby obtain instruments 3-D position and orientation. After the coordinate transformation and a series of orientation calculation, operation navigation comes true.

运用两个摄像机对同一目标从不同位置成像,进而从视差中恢复距离;通过对手术现场的仔细了解与手术医师的反复商榷,使用LED作为标志点,利用非接触式光学定位方法提取空间点位置信息:通过对摄像机的标定、图像处理及目标点重心的计算,求出空间点在虚拟世界坐标系下的三维坐标,从而确定了手术器械等的空间位姿,通过空间坐标转换及一系列定位计算,完成了手术导航实验系统。

The quality of commercial Mei-Gin products are different from each other XVI and shows unsatisfied results. however, In future, possibly, HMF can be applied as a bioactive standard marker for quality control of Mei-Gin. Based on the qualitative and quantitative analysis of HMF, we can establish the model of quality control, and find out facilitative, efficient, and representative chemical fingerprint for commercial Mei-Gin products. Standardizng the Mei-Gin product from the same procedure will bestead the market of plum in Taiwan. Not only advancing the economic value of plum, but also accelerating the competitiveness in marketplace. Establishent of quality control model with HMF as a bioactive standard marker will be crucial issue in the future.

目前在台湾,梅产品还没有一个可以判断品质优的标准,市面上的产品品质也是差齐,在品质管制方面一直无法获得人满意的成果,透过HMF 的定性定分析,可确初步品质管制的模式,为梅产品订出一个简有效且具代表性的化学指纹图,使所有同一产程的产品一致化以及标准化,这将对於梅子产业有相当大的助,仅可以提升梅子的经济价值,并且增加在市场上的竞争,因此用HMF 作为Bioactive standard marker,并且为梅产品建起品质管制模式在未将会是相当重要的研究之一。

Concept of culture Shenzhen is a city of speed and we can make a response with the marker's external form and composite streamline system; Shenzhen is a city of diversity and the marker's performance at different visual angles and function composition is its external manifestation; Shenzhen is a city of innovation, marker is the external form and composite function carrier of its urban wonders, a brand-new experience in behavior is triggered when a super strong attraction is generated.

文化 深圳是个速度的城市,我们通过标志物外在形式及复合流线体系予以回应;深圳是个多样的城市,标志物不同视角的演绎与功能复合是其外在体现;深圳是个创新的城市,标志物以其城市奇观外在形式与复合功能载体,产生超强行为吸引力的同时触发全新行为体验。

An RAPD marker was tightly linked to Fulvia fulva resistant gene Cf6 in tomato, with a genetic distance of 8.7 cm, The RAPD marker was converted into SCAR marker.

经F2单株分析,在后代群体中扩增出了两条长约500 bp的特异片段,该特异标记与叶霉病抗性基因Cf6紧密连锁,遗传距离为8.7 cm。

The method is simple, has safe operation, creates an approach utilizing a plant gene to safely select the marker gene for the tomato genetic transformation, can replace the disadvantages caused by the widely used heterogenous genes such as antibiotic genes, weedicide resistant genes, xyl A, pmi, GUS and so on, taken as marker genes, obtains a transgenic tomato with food safety, biological safety and ecological safety, relieves the public from anxiety of marker gene safety, and inevitably facilitates the commercialized application of the transgenic tomato.

该方法简单,操作安全,开创了利用植物基因作为番茄转化安全筛选标记基因的途径,能够代替目前广泛使用的抗生素基因、抗除草剂基因以及xylA、pmi、GUS等异源基因作为标记基因存在的弊端,获得具有食品安全性、生物安全性和生态安全性的转基因番茄,解除公众对标记基因安全性的顾虑,必将促进转基因番茄的商业化应用。

Moreover, CoI gene was more conservative and a lower evolutionary rate than Cyt b gene. CoI gene was an effective marker in analysing the phylogenesis of families in Passeriformes. It can be used in identifying the species of Passeriformes, but it was revealed that CoI gene was more suitable to identify the phylogenetic relationship of avian family unit than Cyt b gene, and CoI gene can be a molecular marker to identify avian species. But in species identification, CoI gene was less stable and accurate than Cyt b gene. We suggested youd better employ the other marker if you have the CoI gene only.(2) In the phylogenetic trees of birds from Lanius, L.

比较分析了雀形目6科15种鸟类的细胞色素b全序列和CoI基因部分序列,结果显示细胞色素b和CoI基因序列的变异位点分别为454个和366个、简约信息位点为337个和303个,而且线粒体CoI基因比细胞色素b基因略微保守、进化速率也较低;CoI基因在确定雀形目科级阶元之间的系统发生关系方面是一种有效的分子标记,同时它也能够用于雀形目鸟类的物种鉴定,但在物种鉴别方面不如细胞色素b基因稳定、准确。

Results:①As for slow and quick transferring methods, the strips of prestained marker in nitrocellulose membrane were stronger than those of nylon and PVDF membranes, and the face and reverso sides of nylon and PVDF membranes were easily confused;②The picture of PVDF through DAB method was slightly clearer than that through nitrocellulose and nylon membrane, and the quick transferring strips were not so regular as the slow transferring strips.

结果1。在快慢转法中硝纤膜的蛋白预染marker条带略强于尼龙膜,而尼龙膜又略强于PVDF膜; PVDF膜和尼龙膜的正反面易于混淆,而硝纤膜的正反面不易混淆。2。

According to the Marker (EcoR1-HindⅢ), the size of the green peach aphid's genome DNA is about 21 kb.

根据分子量Marker(EcoR1-HindⅢ),桃蚜基因组DNA 的大小在21kb 左右。

It is difficult to think of a test or marker system that would not first be developed, in some form, in an animal model.

以上这句实在搞不清楚应该怎么翻,请指教!marker system前文有提及,此处翻成标记系统即可。

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