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Experimental results based on backcross population indicated that the sensitivity and specificity of PCR marker were almost as same as that of RFLP marker.

回交后代的鉴定结果表明,该PCR标记的灵敏度和专化性与RFLP标记基本相同。

In this essay, we summarized the characteristics of molecular marker-assisted selection, focused on improving qualitative and quantitative characters, backcross breeding and gene polymerization. At the same time, the problems and potential prospects of molecular marker-assisted selection have also been discussed.

综述了分子标记辅助选择的特点,重点介绍了分子标记辅助选择在水稻育种上的利用现状,主要包括质量性状改良、数量性状改良、回交育种、基因聚合等方面的应用进展,同时讨论了该技术存在的问题以及发展前景和展望。

The quality of commercial Mei-Gin products are different from each other XVI and shows unsatisfied results. however, In future, possibly, HMF can be applied as a bioactive standard marker for quality control of Mei-Gin. Based on the qualitative and quantitative analysis of HMF, we can establish the model of quality control, and find out facilitative, efficient, and representative chemical fingerprint for commercial Mei-Gin products. Standardizng the Mei-Gin product from the same procedure will bestead the market of plum in Taiwan. Not only advancing the economic value of plum, but also accelerating the competitiveness in marketplace. Establishent of quality control model with HMF as a bioactive standard marker will be crucial issue in the future.

目前在台湾,梅产品还没有一个可以判断品质优的标准,市面上的产品品质也是差齐,在品质管制方面一直无法获得人满意的成果,透过HMF 的定性定分析,可确初步品质管制的模式,为梅产品订出一个简有效且具代表性的化学指纹图,使所有同一产程的产品一致化以及标准化,这将对於梅子产业有相当大的助,仅可以提升梅子的经济价值,并且增加在市场上的竞争,因此用HMF 作为Bioactive standard marker,并且为梅产品建起品质管制模式在未将会是相当重要的研究之一。

In bivalve molluscs, related phenomena, marker-associated heterosis and distortion of marker segregation ratios, have been widely reported over the past 25 years.

在双壳贝类研究中,与标记相关的杂种优势及偏分离现象在过去的25年中被广泛报道。

Typical bands were used to analyze relation with ecotypes and characters of fruits, indicating that there was no relation between typical bands and ecotypes, but some extent relation between bands and characters of fruits existed. If all cultivars were divided into freestone and clingstone, melting and nonmelting, both freestone (ratio shared total survey, 41/56=73.2%) and clingstone (ratio shared total survey, 33/79=41.8%) appeared in the loci of S_(167)-1050 bp linked marker raised by Warburton et al. It was apparently not strong linkage between the marker and the character, and exchange rate of genes was high. If combinations of S_(167)-850bp and S_(167)-1050bp or S_(167)-850bp and S_(167)-1400bp were used to distinguish characters of fruits, 6 freestone accessions without clingstone ones and 6 melting accessions without nonmelting ones displayed in the first combination of markers, 20 clingstone accessions and 2 semi-freestone ones without freestone ones appeared in the second combination of markers.

通过典型带对不同生态型、果实性状为类别进行分析,结果未发现有与生态型相吻合的特征带出现,而将供试所有品种按离核、粘核和溶质、不溶质划分归类,供试品种在Warburton等得到S_(167)-1050 bp处粘离核分子标记,出现既有离核(占供试离核41/56=73.2%),又有粘核(占供试粘核33/79=41.8%),可见此连锁标记连锁性不强,发生交换的机率较高,而S_(167)-850bp带和S_(167)-1050bp带同时出现时,该组包含6个离核,无粘核品种;6个溶质,而无不溶质。S_(167)-850bp和S_(167)-1400bp同时出现时,包含20个粘核品种和2个半离核品种。

Based on the sequence of the codominant RAPD marker〓,we designed SCAR primers SCN11 to amplify distinct band〓 with the same size as RAPD marker 〓.

根据克隆片段〓和〓的序列设计合成了一对SCAR引物SCN11,SCN11在95-5383中扩增出与〓大小相同的片段〓,在HB1中扩增出与〓大小相同的片段〓,在F1同时扩增出〓。

A 1172 bp fragment was detected for Wx-7A gene using dominant marker primer from the Wx-A1 normal wheat, a 593 bp and a 574 bp fragment were detected using codominant marker primer from the Wx-A1α normal and Wx-A1 null wheat, respectively.

STS标记结果中,Wx-7A位点的显性标记引物在野生型中扩增出一条1172bp的特异带,即Wx-A1α基因出现;Wx-7A位点的共显性标记引物在野生型中扩增出一条593bp的特异带,在缺失类型中扩增出一条574bp的特异带。

There are 2 alleles at a marker locus and they are codominant. We assumed that the sires used were heterozygous at all marker loci with known linkage phases and were mated to females which are randomly sampled from a population being in HardyWeinberg equilibrium.For further understanding of the values of treating QTL effect as random effect and the importance of estimating the variance component of QTL, we estimated the additive QTL effect under several factors combinations assuming that the QTL vanance component to be known.

为了进一步明确将QTL效应作为随机效应处理,并估计其方差组分的重要意义,本文在方差组分真值给定的前提下,采用随机QTL效应模型和单标记及侧翼标记信息,研究了不同试验设计、不同群体结构、不同QTL等位基因类型、不同遗传力和不同QTL方差贡献等因素组合下,TM-BLUP种畜遗传评定的效果。

Compared the VOCs in lung cancer patients and normal people and metabolic product of lung cancer cells to analyze the correlation between lung cancer and its marker VOCs.Results 7 lung cancer patients were detected three marker VOCs in their breath: Benzene,Propyl benzene and Decane.

在肺癌患者的呼吸气体中所含的特征性有机成分的检测方面[1~3],已经有部分科研人员做了尝试,也有培养肺癌组织细胞并探测其代谢气体成分的研究[4],期望能建立通过检测患者的呼吸气体即能够初筛肺癌患者的简便方法。

Vector pDLBRBNPT contained plant selective marker gene nptⅡ and vector pCDMAR-Hyg contained plant selective marker hygramycin phosphotransferase gene , they fitted to dicotyledon and monocotyledon transformation respectively.

载体pDLBRBNPT含有植物选择标记基因nptⅡ,适合双子叶植物转化;而载体pCDMAR-Hyg的植物选择标记潮霉素磷酸转移酶(Hygramycinphosphotransferase,Hyg)分离,主要用于单子叶植物转化。

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