查询词典 isolate

In order to isolate specific functional genes involved in reproduction and endocrine regulation and to reveal their evolutionary mechanisms in Chinese sturgeon, a chondrostean fish with a history of 140 million years, we constructed its pituitary cDNA library from a 4 year-old male.

为了能在中华鲟中发现与生长和生殖内分泌调控相关的基因，我们构建了中华鲟垂体的SMART cDNA质粒文库。

The algorithm needn't generate candidate itemsets and scan database, because it employs FP-Tree to store trasanctions in database compressedly and takes full advantage of the characteristic of FP-Tree.Consequently,the complexity of space and time the algorithm used is reduced obviously in the procedure of data mining.In a thorough experimental analysis of our algorithm on real and synthetic data,we isolate the effect of the individual components of the algorithm. Our performance numbers show that MFP-Miner outperforms previous work.

该算法利用频繁模式树来压缩存储数据库中的事务，并充分利用频繁模式树的特点，在挖掘最大频繁项目集的过程中不需要产生候选项目集和扫描数据库，从而减少了算法在挖掘过程中使用的存储空间和计算时间，通过实验结果显示，该算法的性能优于同类算法。

Contrarious moods of men recoil away;And isolate pure spirits, and permit

一个化学家能将水中的氧与氢分解出来。

Let us stay rather on earth, beloved, where the unfit contrarious moods of men recoil away, and isolate pure spirits, and permit a place to stand, and love in for a day, with darkness and the death hour rounding it.

在这里，人晦涩的对抗情绪会畏缩，会分离出纯洁的精神，会有个地方可以容身并且相爱，那怕一天也罢，哪怕一天之后便是一片黑暗，到了死期。

Contrarious moods of men recoil away;And isolate pure spirits, and permit

他在难以作出决定时往往踌躇不前。

Extraction of high-quality RNA from Panax ginseng tissues is particularly difficult due to high levels of polyphenolics and polysaccharides, or other compounds that bind and/or coprecipitate with RNA. The methods such as guanidinum thiocyanate, CTAB and modified Trizol were applied to isolate high purity and integrity RNA from Panax ginseng.

针对人参组织多酚、多糖类物质含量较高的特点，比较了改良的异硫氰酸胍法、改良的CTAB法和改良的Trizol法等3种不同的RNA提取方法。3种改良的方法均能从人参组织中提取到总RNA。

In order to isolate the active compounds in ethyl acetate fraction of Psoralea corylifolia L., activity-guided isolation was performed along with chromatographic techniques. Four active compounds were isolated from ethyl acetate fraction and identified by their NMR spectral data and physical-chemical properties. They are corylin and bavachin, which showed stimulating effect on osteoblastic proliferation and differentiation, isopsoralen and psoralen exhibited activity of promoting cell differentiation in some degree.

为了追踪分离补骨脂乙酸乙酯萃取物中的活性成分，本实验采用多种色谱分离技术，以活性测试为导向，结合波谱技术及理化数据，从补骨脂乙酸乙酯萃取物中分离并鉴定了4个活性化合物的化学结构，它们是具有促进细胞增殖和分化作用的补骨脂异黄酮和补骨脂二氢黄酮，对细胞分化有一定促进作用的异补骨脂素和补骨脂素。

Methods: The olfactory nerve layer and glomerular layer was isolated from the olfactory bulb of three-day old rats, Enzyme digestion method was used to isolate cells and the cells were purified by differential adhesion method. Freshly isolated OECs were plated onto poly-L-lysine-coated tissue culture and then incubated for 2 days. The purity of OECs was identified by immunocytochemistry of NGFRp75 and S100 and Hoechst33342 counterstain method.

从新生SD大鼠(3d)嗅球中迅速分离嗅神经层和嗅颗粒层，采用酶消化法分离细胞，差速贴壁法纯化细胞，接种于多聚赖氨酸包被的培养板内培养2d，采用NGFR p75和S100蛋白双标免疫组化、以Hoechst33342复染鉴定OECs的纯度。

On the other hand, we tried to isolate heavy metal tolerance genes with novel function in higher plant Cynodon dactylon.. The functional complementation of a yeast mutant strain M379/8, which was sensitive to heavy metal, Cd, was carried out.

木文还尝试了利用酵母重金属敏感突变株M379／8功能互补的方法从重金属抗性植物绊根草中分离新的重金属抗性相关基因。

Methods Five different ultraviolet absorption components were isolated from the filtrate of Botrytis cinerea isolate 7–3 culture. Of the five components, one showed strong inhibitory to Digitaria sanguinalis.

在前期薄层层析法、柱层析法和高效液相色谱法分析的基础上对灰葡萄孢诱变菌株BC7–3的代谢产物中具有除草活性的5个不同组分分别进行了液相色谱制备。

Chinese traditional virtue is humility and wariness,the compliment and praise to the others should be declination

中国人的传统美德是谦虚谨慎，对别人的恭维和夸奖应是推辞。如

We bought this house on the never-never.

我们以分期付款的方式买下这座房子。