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Methods Autologous cord blood serum was used to culture cord mesenchymal stem cells. An enzyme digesting method was used to isolate mesenchymal stem cells from the cord. Morphology, immunotyping and hepatogenic differentiation of these cells were assayed.

分离自体脐血清用于后续细胞培养，利用酶消化法从脐带组织中分离培养间充质干细胞，检测其形态和免疫表型，并采用两步法诱导其向肝细胞诱导分化。

Because more than 75. 4% regions were occupied by heterochromatin on the sex chromosomes of spiny eel, its X library should be a good tool to isolate the heterochromatic highly repetitive sequences DNA and study its origin and evolution.

在刺鳅的性染色体上，75.4％以上的长度都是异染色质区域，因此，刺鳅X染色体文库也为分离获得鱼类性染色体上异染色质重复序列，探讨其起源和进化规律提供了良好的实验材料。

Rep-PCR method was used to conduct cluster analysis to the 39 bacterial isolates. rep-PCR analysis revealed that the 39 bacterial isolates could be divided into 12 clusters at the 0.9 dissimilarity percentage level. The 39 bacterial isolates had their own specific band patterns, showing obvious genomic heterogenicity and rich genotypic polymorphism genetic diversity between each isolate.

采用rep-PCR技术对39个细菌分离株进行聚类分析，在rep-PCR图谱的相异百分数为0.9的水平上被聚类为12个类群，39个分离株各自具有独特的带型，说明各细菌分离株的基因组之间存在显著异质性，表现出丰富的基因型多态性。

To enrich and isolate different function hydrocarbon-degrading bacteria from long-term oil-contaminated environment, crude oil and hexadecane were used as sole carbon sources respectively. On the other hand, bacterium community structures of enrichment were analysed by both plate method and PCR-DGGE (denaturing gradient gel electrophoresis) method.

中文摘要：以原油和正十六烷为唯一碳源，从长期受石油污染的环境中富集和分离具有不同功能的石油烃降解菌，并利用平板法和PCR-DGGE法对不同碳源富集到的菌群结构进行分析。

Differential hybridization was used in this experiment to isolate genes expressed in hyphal from specifically.

分别以白色念珠菌菌体和菌丝mRNA为模板合成探针，进行差别杂交筛选。

Immunoprecipitation and matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) were used to isolate and identify proteins which can interact with interceptive AIF respectively.

提取转染截断型AIF基因的细胞的蛋白质，用免疫沉淀方法分离与截断型可能存在相互作用的蛋白质，用基质辅助激光解析电离飞行时间质谱对分离得到的蛋白质进行鉴定，其中一蛋白质条带经鉴定为β-actin。

We introduced improved primary mixed glial culture and different-attachment method to isolate and purify the OPCs, the cells were proliferated in serum-free medium, flow cytometry and immunohischemistry methods were employed to estimate the purity of cultured OPCs. Their abilities of differentiation and expression of trophic factors were identified by RT-PCR and immunostaining. Several methods including TUNEL and MTT were adopted to estimate the protective effects of conditioned culture medium from oligodendrocyte lineage cells on the primary cultured cerebellar granule neurons. Intravitreal transplant of OPCs, combined with retrograde fluorescent labeling the superior colliculus and intraorbital optic nerve transection, were used to investigate the protective effects of OPCs on the axotomized RGCs in vivo. Intravitreal transplantof OPCs or NSCs on the newborn rats, and retinal transplant of OPCs on the young rats were performed, to observe the myelin formation in the retina at different stages after cellular transplantation. Optic nerve transection was carried out on some rats with myelinated retinae, to study the influence of myelination on the injuried RGCs.

为此，本研究采用改良的胶质细胞混合培养与差速贴壁方法获得大鼠OPCs，使用无血清培养基进行扩增、培养，用免疫组织化学和流式细胞技术对培养细胞的纯度进行鉴定，对少突胶质系细胞表达部分营养因子的情况进行检测；采用TUNEL、MTT等方法对少突胶质系细胞条件培养基对原代培养小脑颗粒神经元的保护作用进行检测；将OPCs移植入成年SD大鼠玻璃体内，利用上丘逆行荧光标记技术，观察眼内移植的OPCs对眶内视神经切断时的视网膜神经节细胞的保护作用及其持续时间；将OPCs或NSCs移植入新生和幼年SD大鼠玻璃体或视网膜内，观察不同时期视网膜内髓鞘形成与分布特点，分析髓鞘的超微结构，并观察眼内髓鞘形成对损伤神经节细胞的保护作用。

Objective To isolate and identify the chemical constituents in leaves of Isatis indigotica Fort.

摘要目的对十字花科植物菘蓝（Isatis indigotica Fort。

A modification of a twostep perfusion protocol and isopycnic centrifugation were used to isolate oval cells from livers.

经门静脉灌注消化法和等密度离心法分离卵圆细胞，并在体外进行长期培养。

A modification of two-step perfusion protocol and isopycnic centrifugation were used to isolate oval cells from liver. Primary oval cells were cutured in LE medium in vitro.

经门静脉灌注消化法和等密度离心法分离卵圆细胞，并在体外进行原代培养。

Objective:To compare the response control and attention of Schizophrenic patients with that of the healthy controls by Integrated Visual and Auditorycontinuous performance test. To quantitate the impairment of cognitive function in patients, and to explore the relationship between cognitive function and the severity of the disorder.

目的：探讨精神分裂症病人在IVA持续操作测试中的反应控制能力、注意力等，并与健康人进行比较，从而量化精神分裂症病人的认知功能损害，并进一步探讨其认知功能与疾病严重程度的关系。

Main effective factors including subcooling degree, mechanical vibration, gas hydrate reformation, environment temperature, noncondensing gas and surfactant are analyzed.

指出过冷度、机械振动、重复生成水合物、环境温度、不凝性气体、添加剂是影响气体水合物生成的主要因素，还对R152a水合物的放冷进行了实验研究。