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This helped them to isolate the 0.3% of cells in mouse testes that are stem cells.

这有助于他们把老鼠睾丸中占细胞比例为 0.3%的干细胞隔离出来。

The observation also indicated the important influence of pH on the growth of the bacterial population, and their quantitative distribution on the glacier surface. The sequence analysis indicated that the obtained DNA fragment from the thermophile isolate Staphylococcus equorum Muzt-D84 belonged to the partial of cspA gene sequence.

结果表明：慕士塔格冰芯中存在3种类型的微生物，其中嗜冷菌为Muzt-D8，其生长温度范围为0~23℃，最适生长温度为15℃；耐冷菌为Muzt-D7， Muzt-G32和Muzt-F01，其生长温度范围为0~40℃，最适生长温度为23℃；宽温菌为Muzt-D84，其生长温度范围为0~45℃，最适生长温度为30℃。

Grass carp reovirus was isolated from outbreak grass carp fingerling in South China freshwater region, and Threadfin reovirus is a Singapore isolate from acute diseased marine threadfin fingerling.

草鱼呼肠孤病毒(Grass carp reovirus，GCRV)是引起中国南方淡水养殖草鱼暴发性出血病病原，鲅鱼呼肠孤病毒(Threadfin reovirus，TFV)是引起海水养殖鲅鱼病毒病病原。

It is also an ideal source of tissue engineering and cell substitutive therapy of regenerative medicine. The target of this experiment is to investigate how to isolate totipotent mouse ESCs from BALB/C species mice and to analysis some biological characteristics of ESCs.

本实验旨在研究如何从BALB／C小鼠的囊胚中分离出具有全能性的胚胎干细胞，并对胚胎干细胞集落进行培养、鉴定，以了解胚胎干细胞生物学特性，为掌握小鼠ESCs分离培养技术及将来进一步研究人ESCs打下基础。

In recent years, gene trapping mediated by T-DNA has provided a powerful tool to isolate and identify genes.

T-DNA介导的基因诱捕技术是近年来发展起来的鉴定和分离基因的方法。

objective to isolate and purify the antimicrobial peptides with anti-toxoplasma gondii activity isolated from the haemlymph of musca domestica larvae.methods the antimicrobial peptides of musca domestica larvae were induced by infection and injury were isolated and purified by trituration,centrifugalization and column chromatography.then the antimicrobial peptides with anti-toxoplasma activity were sieved by mtt colorimetric method and haemacytometry.results it was found that two antimicrobial peptides had anti-toxoplasma activity through resource s cationic column chromatography and superdex g75 gel column chromatography.conclusion the two antimicrobial peptides with anti-toxoplasma activity existing in the haemlymph of musca domestica larvae had different anti-toxoplasma effect.

作者单位：山西医科大学寄生虫教研室，太原 030001；化学生物学与分子工程教育部重点实验室山西大学生物技术研究所目的从家蝇幼虫血淋巴中分离纯化具有抗弓形虫作用的抗菌肽。方法通过损伤加感染的方法诱导家蝇幼虫大量表达抗菌肽，然后经过研磨、离心和层析等过程，将家蝇幼虫抗菌肽进行分离纯化，采用四甲基偶氮噻唑蓝比色法和血细胞计数法筛选对弓形虫速殖子有抑制作用的抗菌肽。结果经resource s阳离子柱和superdex g75凝胶柱层析后，筛选出2种对弓形虫速殖子有杀伤作用的抗菌肽。结论家蝇幼虫血淋巴中存在抗弓形虫作用的抗菌肽，而且不止一种，但其效果有所不同。

To isolate mononuclear cells from foetus umbilic cord blood, After MNCs were incubated in the presence of APS group (APS 200 mg·L-1) and control group for 24 h,the cells were stimulated with Epo（5 IU/mL） for 0,2,5,30 min; STAT5 was measured by ICC and laser confocal scanning microscope, JAK2、STAT5 in nucleus and cytoplasm were measured by western-blotting-ECL in each group at different groups.

分离纯化胎儿脐带血单个核细胞，在常规体外培养体系加入APS（200 mg·L-1）作用细胞24 h，促红细胞生成素分别刺激0，2，5，30 min，免疫细胞化学与激光共聚焦显微镜观察细胞STAT5的表达；Western-blotting-增强化学发光法检测细胞JAK2与浆和核中STAT5的表达。

OBJECTIVE: To isolate MSCs from human umbilical cord blood, and to detect their biological properties.

目的：拟从人脐带血中分离培养间充质干细胞，并对其生物学特性进行检测。

Based on modified whole genome PCR and electrophoretic mobility shift assay , our laboratory established a new method to isolate genomic regulatory sequences using unpurified nuclear extract.

本实验室在对原有的全基因组PCR技术进行改进的基础上，结合凝胶阻滞实验，建立了筛选DNA调节片段的新方法。

To establish a new method to isolate genomic regulatory sequence using unpurified nuclear extract, here a new strategy was developed based on modified whole genome PCR and electrophoresis mobility shift assay. The principle is as follows. After sonication and ligation with DNA linker, human genomic DNA is amplified and labeled with isotope by whole genome PCR.

为了建立一种利用未经纯化的细胞核粗提物就能从人基因组中快速、大量筛选DNA调节片段的新方法，本研究对原有的全基因组PCR技术进行改进，结合凝胶阻滞实验，建立了筛选DNA调节片段的新方法。

Objective:To compare the response control and attention of Schizophrenic patients with that of the healthy controls by Integrated Visual and Auditorycontinuous performance test. To quantitate the impairment of cognitive function in patients, and to explore the relationship between cognitive function and the severity of the disorder.

目的：探讨精神分裂症病人在IVA持续操作测试中的反应控制能力、注意力等，并与健康人进行比较，从而量化精神分裂症病人的认知功能损害，并进一步探讨其认知功能与疾病严重程度的关系。

Main effective factors including subcooling degree, mechanical vibration, gas hydrate reformation, environment temperature, noncondensing gas and surfactant are analyzed.

指出过冷度、机械振动、重复生成水合物、环境温度、不凝性气体、添加剂是影响气体水合物生成的主要因素，还对R152a水合物的放冷进行了实验研究。