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4The reproductive activity are fairly between the adipose mesenchymal stem cells and bone marrow mesenchymal stem cells, the postdigestive 2nd and 5th generation cells have 6-9 days log phase growth after the latent period about 24-48 hours, then transit to the platform period.

4脂肪间充质干细胞的增殖能力与骨髓间充质干细胞相当,消化后的第2、5代细胞经历24~48小时的潜伏期后,进入6~9天的对数生长期,然后过渡到平台期。

BACKGROUND: At present, studies show that a kind of stem cell community which in many kinds of organizations can differentiate into tissue cells of different embryonic layers; but those are different from embryonic stem cells, embryonic stem cell will lose the part differentiation potential gradually during the development of pregnancy, and will present some special phenotypes or the molecular markers, as CD105 and so on, will call it postembryonic pluripotent stem cells.

背景:目前研究认为在胚胎发育后的多种组织中存在一类干细胞群体,可分化为不同胚层的组织细胞;但又不同于胚胎干细胞,随着妊娠期间胚胎的发育胚胎干细胞会逐渐失去部分分化潜能,会出现一些特殊表型或分子标记,如CD105,称其为胚胎后亚全能干细胞。

All 31 YSTs (5 pediatric and 26 postpubertal) showed strong positive SALL4 staining in more than 90% tumor cells but had negative OCT4 staining. Both spermatocytic seminomas showed positive SALL4 staining in 80% to 95% tumor cells in all 3 types of tumor cells with weak-to-moderate staining intensity.

为了检验SALL4的特异性,我们还给23例睾丸的非GCTs(10例间质细胞瘤、4例支持细胞瘤、3例腺瘤样瘤、3例睾丸旁横纹肌肉瘤、2例弥漫性大B细胞瘤、1例睾丸网的乳头状囊腺瘤)和275例非睾丸性肿瘤(158例转移癌、12例转移性黑色素瘤、11例原发性和2例转移性间皮瘤、72例原发性和20例转移性肉瘤)做了SALL4免疫染色。

BACKGROUND: The precartilaginous stem cells are limited regarding in vitro proliferative capacity, but the immortalized cell lines can provide a large number of stable immortalized cells, and simian virus 40 large T antigen gene (SV40Tag) is one of gene fragments which are commonly used and effective in vitro immortalized cells.

摘要背景:前软骨干细胞体外培养增殖能力有限,但永生化细胞株可以提供大量性状稳定的永生化细胞,并且猿肾病毒40大T抗原基因(simian virus 40 large tumor antigen,SV40Tag)是较为常用且有效的体外永生化细胞的基因片段之一。

[Objective]To construct eukaryotic expression vector containing Sox9 derived from immortalized precartilaginous stem cells and to probe its inductive effect on marrow stroma cells differentiating into precartilaginous stem cells.

[目的]从永生化骨骺干细胞中克隆Sox9基因并构建真核表达载体,并探讨Sox9诱导骨髓基质细胞向骨骺干细胞分化的可能性。

Ray width 1-4 cells; Body ray cells procumbent with 1-9 rows of upright and/or square marginal cells.

射线宽1-4细胞;多列射线的中部多为横卧细胞,边缘有1-9行直立和/或方形细胞。

Ray width 1-4 cells; Body ray cells procumbent with 1-4 rows of upright and/or square marginal cells.

射线多列和单列射线组成,两种大小,1-4细胞宽。

The results showed that the non-embryonic callus were cultured on the MS medium contained 4.0 mg · L-1 2,4-D and 0.5 mg · L-1 6-BA, embryogenic cells appeared in it. Then the single embryogenic cells formed a proembryo composed of many cells through cell division.

结果表明,非胚性愈伤组织转入到MS + 4.0 mg · L-1 2,4-D + 0.5 mg · L-1 6-BA培养基培养后,愈伤组织中先出现一些胚性细胞;然后单个胚性细胞分裂形成一个由多细胞组成的原胚。

Most techniques in vivo monitoring the distribution, prolification and migration of transplanted cells. To objectively evaluate curative effect after cells transplantation, most techniques for the study of stem cell transplantation in animal models require histological analysis to determine the fate and migration of cells.

监测活体内移植入细胞的分布、增殖、迁移的方法虽然有多种,但传统方法都需要在离体状态下进行组织学切片分析和鉴定,无法准确地观察植入细胞的生长分化情况,从而难以对干细胞移植术的疗效作出客观评价。

Among its many biological activities, the major function of GM-CSF is to stimulate hematogenie stem cells prolification from silent stage to S phage, so that the stem cells can differentiate into granulocyte-macrophage cells.

有4个外显子和3个内含子,基因定位于5号染色体长臂5q21-5q32区,大多数器官均能产生GM-CSF,GM-CSF有多种生物活性,作用于多种细胞谱系,其主要作用是刺激造血祖细胞增殖即从静止期进入S期,并分化为粒细胞---单核巨噬细胞。

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Effective local search tactics reaches local searchers when they are ready to buy - with every way that it is possible for them to buy from you.

有效的地方搜索策略达到当地的搜索,当他们准备购买-以各种方式,这是他们有可能从您那里进行购买。

The first two are periodic sentences for their beginnings; and the fifth and the last are also periodic sentences each for the embedding of a parenthetic structure.

1960年代中后期,当战后女权主义和当时的许多其他抗议运动在西欧和北美发展成某种势力时,性别的概念演绎成补充和延伸生物性别差异的概念。

I habe been staying here for 20 years.

我在这里二十年了,岁月,生活