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The result is consistent with previous studies. Whereas, tapetal cells of sterile anthers were disintegrated since tetrad cells were disjoined, indicating that disintegration of tapetal cells is earlier occurred in sterile anther than that in fertile anther. DNA ladder revealed that the cleavage of nuclear DNA into oligonucleosome-sized fragments to

结果表明，在花粉母细胞减数分裂后的四分体解离时期，不育花药的绒粘层细胞较可育花药提前开始裂解；在不育系S-Mo17rf3rf3花药和花粉S-(rf3)中均明显出现PCD过程的DNA片段化以及线粒体细胞色素C外渗的现象，证明了玉米CMS-S的花粉败育与花药绒粘层细胞的提前凋亡和小孢子细胞的细胞程序性死亡有关。

Results Smad4 genes were expressed throughout the postnatal cochlea of three mouse genotypes at a high level. The expression concentrated in the vascular stria, spiral ligament, basal membrane, tectorial membrane, hair cells, supporting cells and spiral ganglion cells.

结果 Smad4在三种基因型小鼠耳蜗均有广泛表达，表达部位主要集中于血管纹、螺旋韧带、基底膜、盖膜、毛细胞、支持细胞、螺旋神经节细胞等处，其中血管纹和基底膜表达最为明显。

As the first attempt, it was designed to reveal the general pattern of lectin receptor distribution on the surface of female cells, namely egg cells, central cells and synergids of Torenia fournieri and, especially, toensure the possible effects of cell isolation procedure on the distribution of lectin receptors by four different systems.

在烟草活体的中央细胞及卵细胞表面发现了伴刀豆凝集素受体的极性分布或者称之为&镶嵌性分布&。在大部分的中央细胞膜表面，该凝集素受体主要分布在靠近核的部位，而在相对侧的荧光要弱或缺失。

Reactive oxygen species causing DNA oxidative damage comes from two kinds of ways:one is from cellular normal physiological metabolism;the other is from outer environment.Redox-sensitive green fluorescent protein was expressed in Saccharomyces cerevisiae.Recombinant cells were evaluated in monitoring the changes in the redox state of living cells when challenged with toxicologically relevant metal ions NaAsO_2 or Pb(NO_3)_2 by measuring emission intensity at 510 nm with a Hitachi F6500 fluorescence spectrophotometer,roGFP expressed in yeast responded not only to typical membrane-permeant oxidants H_2O_2 and reductants DTT,but also to toxicological metal ion-induced intracellular redox changes in a dose-dependent manner.Moreover,exposure of yeast cells to NaAsO_2 or Pb(NO_3)_2 at concentrations that induced redox changes reported by roGFP caused up to 2～3 fold increases in DNA mutation frequency.This mutagenic effect was largely caused by oxidative stress since blocking the production of hydryl radicals with thiourea significantly reduced the mutation rate as well as delayed the cell death.

本文将对氧化还原状态变化敏感的绿色荧光蛋白roGFP1-R12，在酵母细胞中实现了多拷贝强表达；荧光扫描经强氧化剂H_2O_2和还原剂DTT以及环境中重金属NaAsO_2或Pb(NO_3)_2处理后的酵母细胞悬液，测定510 nm处的荧光发射强度结果显示，表达的绿色荧光蛋白对氧化还原水平敏感，且在510 nm处的荧光强度与一定的重金属浓度呈正相关，即roGFP1-R12在510nm处的荧光发射值随重金属浓度的增高而增强，从而说明重金属对细胞的毒性在一定程度上很可能是通过破坏细胞内的氧化还原平衡发生作用；同时通过该绿色荧光蛋白对胞内氧化还原状态变化的响应情况可以来实时检测环境中的重金属；遗传学的点突变频率及致死率实验数据表明，重金属能导致菌体的点突变频率和致死率升高，且活性氧的清除剂巯基脲能明显降低这种点突变和致死率，说明由重金属引发的这种点突变和致死效应在很大程度上是依赖于重金属对细胞诱导产生的氧化胁迫。

The rates of cells adhesion, cells motion and cells invasion were correlated with concentration of Trametes robiniophila.

结论］ 槐耳浸膏能明显抑制肝癌细胞的黏附、运动及侵袭能力，故可以阻抑肝癌细胞的转移。

RESULTS: Olfactory bulb-derived olfactory ensheathing cells adhered to the wall at 24 hours. Olfactory mucosa-derived olfactory ensheathing cells adhered to the wall at 4-5 days. The morphology of the two kinds of cells was similar. Most of them were bipolar and spindle-shaped, and few were tripolar and flat.

结果：体外培养24 h嗅球源性嗅鞘细胞即可贴壁，而嗅黏膜源性嗅鞘细胞多在培养四五天后贴壁，两种来源的嗅鞘细胞形态相似，以双极或梭形细胞为主，少量为3极及多突起形多级细胞，同时夹杂扁平、煎鸡蛋形细胞。

Results The olfactory ensheathing cells from human fetal olfactory mucosa were positive for P75 and GFAP, and in in vitro culture, the cells exhibited dipolar or tripolar appearance with long thin neurites. On the 9th day of cell culture, the purity of the olfactory ensheathing cells reached about 83%.

结果 人胚嗅粘膜嗅鞘细胞形态多呈双极、三极，伴有细长的突起。p75和GFAP染色均呈阳性反应，体外培养9d时纯度可达83%。

Dr. Krymskaya found that tuberin plays very similar roles in LAM cells as it does in fly cells, and that an FDA approved drug called rapamycin can mimic the function of tuberin in LAM cells.

Krumskaya博士发现马铃薯球蛋白在LAM细胞中的作用和在果蝇细胞中的作用非常相似，并且FDA已经批准的一种叫做rapamycin的药物能够模拟马铃薯球蛋白在LAM细胞中的功能。

P19 differentiation was induced by retinoic acid, and P19 cells cultured on gelatin were used as control. Differentiated cells were observed with microscope. TURNEL method and DNA fragmentation analysis were used for apoptosis confirmation. Survival cells were detected by AlamarBlueTM method.

视黄酸(retinoic acid，RA)诱导P19细胞分化，培养于明胶包被培养板上的P19细胞为对照组，普通光学显微镜观察其神经分化情况，TURNEL法和DNA片段化分析法检测细胞凋亡情况，alamarBlueTM染色法检测细胞存活情况。

Flow cytometric analysis showed that after mock infection, Ad5null infection, and Ad5hSeFT infection, the mean fluorescence intensity values for the binding of Ulex europaeus Ⅰ lectin to NIH3T3 cells were 2. 3±0.6, 2. 1±1. 0, and 36. 5±5. 9, respectively; MFI values for the binding of Griffonia simplicifolia isolectin B4 (GS-IB4) lectin to NIH3T3 cells were 167±23, 170±19, and 100±14, respectively; MFI values for the binding of human natural IgG and IgM antibodies to NIH3T3 cells were 31±3, 32±4, and 22 ±4, respectively.

流式细胞分析结果表明，小鼠NIH3T3细胞及分别被Ad5null和Ad5hSeFT转导的NIH3T3细胞，UEA-1凝集素结合细胞的平均荧光强度值分别为2.3±0.6、2.1±1.0和36.5±5.9；GS-〓凝集素结合细胞的MIF值分别为167±23、170±19和100±14：人天然IgG和IgM抗体结合细胞的MFI值分别为31±3、32±4和22±4。

Effective local search tactics reaches local searchers when they are ready to buy - with every way that it is possible for them to buy from you.

有效的地方搜索策略达到当地的搜索，当他们准备购买-以各种方式，这是他们有可能从您那里进行购买。

The first two are periodic sentences for their beginnings; and the fifth and the last are also periodic sentences each for the embedding of a parenthetic structure.

1960年代中后期，当战后女权主义和当时的许多其他抗议运动在西欧和北美发展成某种势力时，性别的概念演绎成补充和延伸生物性别差异的概念。