查询词典 insert cells

METHODS:Nestin expression was detected in 33 cases of neuroepithelial tumors using the immunocytochemical staining. The Nestin positive and negative cells were isolated by magnetic activated cell sorting. Nestin positive cells were culutered, identified, and induced to differentiate. The expression of Nestin protein was detected in Nestin+ cells by RTPCR.

采用免疫组化法检测神经上皮肿瘤组织中Nestin的表达情况，免疫磁珠法分选肿瘤组织中的Nestin+细胞，悬浮细胞培养法进行Nestin+细胞的增殖、分化与鉴定，RTPCR法检测Nestin蛋白在其中的活性表达情况。

Human embryonic stem cells can differentiate into neuroepithelial progenitor cells in both feeder containing and feeder free systems,yet feeder free system has a relatively lower inductive efficiency with part of undifferentiated embryonic stem cells.

人胚胎干细胞在有饲养层和无饲养层体系中均可诱导分化形成神经上皮祖细胞，但无饲养层体系的效率相对较低，且有未分化胚胎干细胞残余。

We observed that the nestin positive cells distributed mainly near the internal limiting membrane and mantle layer of neuroepithelium. The positive cells arranged regularly along the neuroepithelial layer. After RA-treated, it was found that the number of neural stem cells reduced, especially at the eighth day of embryo p

RA作用后Nestin阳性细胞数目均普遍低于正常组；尤其在E8.5d时阳性细胞数目减少最为显著 p CDK5相似，于胚胎E14d已见其强阳性表达，成年期、老年期表

Bone marrow stromal cells are cells got rid of hemopoietic stem cells in marrow cell of mature individual, which is approved to be multi-differentiation potential including transforming into neural stem cell, and differentiation into neurs and neuroglial cell.

骨髓基质细胞(Bone marrow stromal cells，BMSCs)是成体骨髓细胞中除造血干细胞之外的另一类骨髓干细胞，近年来的研究证明其具有多向分化的潜能，包括向神经干细胞转化，并可进一步分化为神经元和神经胶质细胞。

Results Triglyceride droplets and giant mitochondrias were observed in hepatic cells, mitochondrias were damaged and lipofuscin increase in neuronic cells,observed apoptosis in both cells.

结果肝细胞内出现大量脂肪滴和巨大线粒体；神经细胞线粒体受损、脂褐质增多；发现凋亡细胞。

However , countless neutrophilic leucocytes were seen in 3 cases(15.8%), plasma cells and reticulum cells in 2 cases (10.5%), and atypical epithelial cells in one case (5.3%) out of 19 cases in remission of ulcerative colitis .

然后，在溃疡结肠炎减轻的19个病例中，无数嗜中性白细胞在3例（15.8%）中可见，浆细胞和网状细胞在2例中可见，不规则上皮细胞在1例中可见。

In addition, under the above-selected nitrosification condition, the immobilized cells N4 and B08 were used for the combined short-cut biodenitriflcation with the ratio of immobilized cells 1:1 and the seed volume 15%, 27h the denitrogenation efficiency reached 97.53%; the immobilized cells N4 and B08 were used to do denitrogenation application test on eutrophic lake water of our school, 12h the NH4+-N removal rate was 84.06%.

在以上所选亚硝化条件下，按1：1的比例，亚硝化细菌N_4和反硝化细菌B_(08)固定化细胞接种量为15％，广西大学硕士学位论文固定化微生物捷径生物脱氮的研究作联合捷径脱氮试验，27h脱氮率为97.53%；并作本校富营养化湖水的脱氮应用试验，处理12h，NH4十一N去除率为84.06%。

Of 92 acutely dissociated dorsal root ganglion cells recorded by current patch-clamp technique, 34% were discharged by FA application with the mean onset latencies of the first action potential being (367.34+/-32.96) s. All the FA-sensitive cells were identified as nociceptor cells by their distinguishable features of AP including longer duration, existence of a hump on the repolarizing phase, and longer after-hyperpolarization of APs.

运用电流钳记录的92个背根神经节细胞中，大约有34%的中、小细胞对甲醛溶液敏感，产生动作电位的发放，其产生动作电位的潜伏期是(367.34±32.96) s，并且单个动作电位复极化相具有伤害性感受器细胞的特征性，如动作电位间期较长、复极化相有&驼峰&以及较长的后超极化相。

The optimal yield of MAPCs was obtained when mononuclear cells were cultured at a initial plating density of 1 × 10~6 cells / cm~2 and the medium was exchanged to remove the nonadherent cells after 72 hours of inoculation.

结果表明，在低血清条件下，DMEM／F12培养基更适合于脐带血MAPC生长；1×10~6／cm~2是原代培养的适宜接种密度，原代第72小时首次换液为最佳换液时问。

Methods: Nonadherent cells from bone marrow or peripheral blood mononuclear cells of 8 patients with chronic myeloid leukemia were cultured with rhGM- CSF and rhIL- 4. The cytokines were supplemented every three days. RhTNF-α was used at the ninth day. All the cells were harvested at the twelfth day.

从8例慢性髓性白血病患者的骨髓或外周血中获取非贴壁细胞，利用细胞因子(rhGM蛳CSF、rhIL蛳4、rhTNF蛳α)联合培养，每3 d补充新的细胞因子，其中rhTNF蛳α为第9天添加，第12天收获细胞。

The role of the environment is very important.

的作用，环境是非常重要的。

At that time, the High Court of England and Wales Court of Queen's Bench president of William Murray, Lord Mansfield in June 22, 1772 ruling:"No matter then there is the inconvenience, but there must be a decision, I can not say that this case under the law in England are permitted or authorized; are so black should be released."

当时的英格兰及威尔士高等法院王座法庭院长William Murray， Lord Mansfield于1772年6月22日宣判：「无论有那麽不便，但总要有个决定，我不能说这件案在英格兰法律之下是准许或认可；所以黑人是应该被释放。