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Acute ablation of Treg cells increased the fraction of IFN-gamma-producing Teff cells, indicating that Teff function was limited by the Treg cells.

急性消融T细胞增加的部分中IFN -γ-生产Teff细胞，这表明Teff功能是有限的T细胞。

Transduction of TNF-α gene into TIL of human glioma by retroviral vectors and study of its biological activity in vitro. The development of retroviral vectors for TNF-α gene transfer and preparation of carrier cells-TIL have al- lowed transducing marked gene NeoR and purpose gene TNF-α into TIL cells respectively by use of monoclone tilter virus PLC-2 and PLJC-5. Detect the proliferation of TIL, expression of TNF-α and the anti-tumor activity of glio- ma TJ8510 cells in vitro.

在TNF-α逆转录病毒转移系统的建立及运载细胞TIL制备的基础上，利用构建的单克隆高滴度病毒细胞株PLC-2和PLJC-5将标记基因NcoR和目的基因TNF-α分别导入到TIL细胞中；对TNF-α基因转导后的TIL细胞进行细胞的增殖情况、TNF-α表达水平及对人脑胶质瘤TJ8510细胞的体外抗瘤活性的检测。

Embryonic stem cells derived from inner cell mass of mammalian blastocyst-stage emyst-stage embryos and primordial germ cells are totipotent cells that can be cultured in virto.

胚胎干细胞是从哺乳类动物的囊胚内细胞群或原始生殖嵴细胞分离、体外培养获得的具有发育全能性的细胞。

AIM To study the isolation and in vitro culture of rabbit s bone mesenchymal stem cells and adipose stromal stem cells and the use of both BMSCs and ASSCs cells in nuclear transfer to produce totipotent blastocysts.

目的 研究非休眠期成年兔骨髓基质干细胞和脂肪基质干细胞作为供体，以体外成熟去核兔卵母细胞作为受体进行核移植构建重组胚胎的可行性及囊胚形成率。

A proportion of the triadiate cells could shorten one arm and gradually pass, during successive divisions into the fusiform cells, however there was little to suggest that fusiform cells ever became triradiate by growing out a third arm.

培养液比重、温度及光照条件的改变短期内对细胞形态类型的转变没有明显影响；淡水和低温(4℃～8℃)的长期胁迫能使梭形和三出放射形藻株发生高比例的卵形细胞。

In liquid culture system of new born rat hepatocyte,thesupernatant and cells are havested respectively every 24h,and theresidue cells are resuspended in fresh medium.The collectedsupernatant and cells are used to the concentration of tyramine byfluorescence assay.

在乳大鼠肝脏细胞的培养体系中，每隔24h收集培养上清并更换新鲜培养液，检测上清中酪胺的浓度；结果显示，每次更换培养液后，都能够在培养24h后的上清中重新检出酪胺，而细胞中检测不到酪胺。

And the effects of supernatant of transfected umb ilical blood cells were investigated alone or in coordinate with GM-CSF by colo ny formation test of human bone marrow cells in semisolid culture.Results SCF mRNA was expressed in transfected umbilical blood cells.

结果 KG*2 转染SCF cDNA的脐血细胞SCF mRNA水平表达量高于对照组，其细胞培养上清协同 GM-CSF刺激骨髓细胞形成集落数比GM-CSF单独作用高两倍左右。

In the current human form, some cells are well fed, other cells underfed, and some not fed at all, which cause such cells to die over time.

在当前人类形体中，一些细胞被良好地供给，其他细胞缺乏供给，而另一些完全没有被供给，其导致了这些细胞随时间死亡。

The citric acid-producing strain Aspergillus niger 2363-2, was isolated and used in our research. The fermentation conditions were optimized as follows: sucrose as carbon source 8. 0-14. 0%, ammonium chloride as nitrogen source 3. 0-4. 0g/l, initial pH 2. 0, and cultural temperature 30℃. The influential factors of formation of spores were also studied, and a series of measures were taken to restrain the spore formation on the basis of our experimental results. With the consideration of the cultural environment and microbial cell itself, the factors affecting the duration of citric acid-producing activity of microbial cells were examined, and it is found that undissociated citric acid and the ageing of microbial cells are the main factors. The experimental results also show that partial replacement of fermentation broth can lengthen the duration of citric acid -producing acitivity of microbial cells.

筛选出了用于试验研究的柠檬酸发酵用菌Aspergillus niger 2363-2，确定了其最适发酵条件—碳源选用蔗糖，浓度8.0~14.0%，氮源为NH〓Cl，浓度3.0~4.0g/l，发酵初始pH值为2.0，培养温度30℃；讨论了分生孢子产生的影响因素，并通过试验提出了抑制分生孢子产生的措施；从分析微生物所处的环境和微生物本身两个方面出发，探讨了影响微生物菌体产酸活性持久性的因素，认为分子态柠檬酸和菌体本身的老化是影响微生物产酸活性持久性的两个主要因素，并提出采用部分置换发酵液发酵能延长微生物菌体的产酸活性。

It indicated that MyoD transfected ES cells can be induced into skeletal muscle cells unexceptionally by DMSO and one can gain plenty of skeletal muscle cells in vitro by this method.

说明通过MyoD基因转染再经DMSO诱导的方法可以从ES细胞获得大量骨骼肌细胞。

Tom: You are right, he worked hard all the time.

你是对的。他一直都是努力工作。

Take shortcomings,failures,and mockings of others as commonplace and a drive to complete oneself,hold your own opinion over the evalution and disscution divested from others,afterwards sink into the state of eliminate mistakes if any or encourge oneself being not being influenced by the sayinngs of others or having no ideas to do.

把缺点、失败及别人的耻笑等看成是一种常事，当成完善自己的动力，对别人的评价和议论自己心中有主见，做到&有则改之，无则加勉&，不为人言所左右或无所适从。