查询词典 genomic

Objective To clone the BP26 gene of B. abortus, highly express in K. coli and purify the expressed product. Methods Extract the genomic DNA of B.

目的 克隆布鲁氏菌BP26基因，并在大肠杆菌中高效表达及纯化BP26蛋白。

In 1997, Baulieu named these steroids as "neurosteroids". In general, through the genomic and nongenomic effects within the CNS, neurosteroids exert a broad spectrum of action on neuronal function and plasticity.

一般说来，通过基因水平和非基因水平两方面途径，神经甾体调节了神经元的功能及其可塑性，在中枢神经系统中起到复杂多样的作用。

During the postgenomics era the information about genomic sequence and gene functions provides a new foundation for evolutionary biology and ecology As the first whole-genome sequenced plant Arabidopsis thaliana and its wild relatives have played a critical role in understanding the evolution of genomics and speciation Both A halleri and A lyrata are closely related to the model species A thaliana A halleri ssp gemmifera occurs in northeastern China Japan and Taiwan; while its sister A halleri ssp halleri is mainly distributed in Europe Geographical barriers such as Tienshan Mountain Range isolate these intraspecific sisters Likewise A lyrata ssp kamchatica and ssp lyrata occur in East Asia and North America respectively Such distribution patterns seem to be consistent with allopartic speciation The comparison between ancestral and extant polymorphism by multilocus can be informative about the population genetics of speciation In this study we collected and analyzed DNA sequences of 98 genes from four wild relatives of A thaliana A halleri ssp gemmifera A halleri ssp halleri A lyrata ssp kamchatica and A lyrata ssp lyrata The ancestral states of these four species were compared to each other in terms of level of genetic variation However the ancestral species at the time of speciation were substantially more polymorphic than the extant geographical populations The observations are not fully compatible with speciation by strict allopatry At some species pairs parapatric speciation seems more reasonable in speciation of Arabidopsis The 98 gene sequences are also used for the congruence test between gene genealogy and species phylogeny Only 28 genes support the species phylogeny but there are 23 genes supports another major genealogy { lyrata} thaliana Based on the phylogenetic position change of A lyrata ssp kamchatica and Ks value for each species pair suggested the recent directional gene flow between A halleri ssp gemmifera and A lyrata ssp kamchatica

阿拉伯芥是第一个完成基因体定序的开花植物，其基因体资讯提供植物学研究的重要依据；在解析阿拉伯芥属物种的亲缘关系以及种化机制等重要的演化议题时，阿拉伯芥近缘的野生物种自然成了不可或缺的关键；跟阿拉伯芥近缘的物种包括A halleri及A lyrata，其中A halleri ssp gemmifera主要分布於中国东北、日本以及台湾，与近缘的A halleri ssp halleri其分布於欧洲隔著天山及大陆的障蔽，而A lyrata ssp kamchatica主要分布於东北亚及台湾，与分布於北美五大湖的A lyrata ssp lyrata被北极圈所分隔，这样的分布模式暗示异域种化的可能。藉由多基因分析比较祖先物种与现生物种遗传歧异度的相关可提供讯息探讨种化时期的族群遗传结构，本研究针对A halleri ssp gemmifera、A halleri ssp halleri及A lyrata ssp kamchatica、A lyrata ssp lyrata四个物种，两对互为亚种的姊妹群，以阿拉伯芥为外群进行研究，在四个物种完成98个同源基因的分子序列，利用套装软体MCMCcoal来估算祖先物种的遗传变异，亦估算现生物种的核苷酸歧异度，观察到？多物种配对中祖先物种遗传多型性大於现生物种DNA歧异度，显示异域种化模型并无法完全解释阿拉伯芥属物种的种化模式，在某些物种配对间邻域种化模式应比异域种化更为可能；在基因树与物种树的比较，98个基因片段的亲缘模式只有28个是与已知物种树一致的，有23个基因其树状图支持{ lyrata} thaliana的型式，藉由kamchatica位置的变化以及估算各物种配对间的平均同义置换率，推测在A halleri ssp gemmifera与A lyrata ssp kamchatica间具有近代的单方向基因交流。

The results indicated that extensive genomic changes occurred at the early stage of allopolyploid genome.

基因组序列变化始于F_1代，变化最剧烈的时期发生在S_0到S_1代。

The genomic changes occurred form F_1 and the greatest changes of sequence was occurred from S_0 generation to S_1 generation,while,there were miner changes from S_1 to S_2, which indicated that the allopolyploid genome became more stable following the selfing process and the sequence change was a rapid event.

但在S_1到S_2代基因组序列变化比率基本接近，暗示了伴随着异源双二倍体的自交过程，早期基因组表现出稳定的趋势，说明基因组序列变化是一个快速的事件。

This study was to explore the relationship of genomes between Oryza alta and Oryza latifolia using genomic in situ hybridization.

［目的］采用基因组原位杂交技术研究稻属2种CCDD基因组型的野生稻宽叶野生稻和高杆野生稻基因组之间的关系。

A pair of specific primers was designed from the sequenceand was used to amplify the genomic DNAs of Yangmai 5,6VS/6AL line,substitution line of 6V and amphidiploid of Triticum durum-Haynaldiavillosa.

根据该序列设计了一对特异引物，分别以感病对照扬麦5号，抗病6VS/6AL易位系，6V代换系，硬粒小麦-簇毛麦双二倍体及簇毛麦的基因组DNA为模板进行特异性扩增。

Studies on sequence changes in the early genomes of interspecific F_1 hybrid and amphidiploid in CucumisIn the present study,extensive genomic changes were detected in newly obtained allotetraploid of Cucumis,namely Cucumis hytivus Chen and Kirkbride,by AFLP analysis using 12 pairs of EcoR-I/Mse-I primers.

甜瓜属远缘杂种F_1及人工异源双二倍体早期基因组序列变化研究利用12对Eco RI/Mse I选择性引物，对甜瓜属人工异源双二倍体C.hytivus及其二倍体父母本进行AFLP分析，结果发现多倍体基因组在形成早期发生了广泛的序列变化。

The genomic sequence of 5'end was obtained and analyzed; however, it seemed that there was no ortholog of the muscle-specific NAC in amphioxus.

对其cDNA 5'端的基因组序列进行预测和比较分析，没有发现肌肉特异性外显子的存在。

Africanum amphiploid (2n=56) using genomic DNA of S.

应加强对非洲黑麦等野生黑麦的研究和利用，进一

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克，果实色泽鲜艳，汁多味浓，甜酸适口，离核仁甜，清末时就驰名陕、甘两省，清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异：红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感，ED90值分别为47.65、71.67和29.17g/hm2；春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感，ED90值分别为96.91、114.20g/hm2；而番茄不敏感。