查询词典 genomic

To evaluate the specificity of the PCR, genomic DNA of Theileria annulata,Babesia bovis,Toxoplasma gondii,Leishmania donovani and standard strain of N. caninum were used as a template in the PCR. For determining the detection limit of amplification procedure, PCR was run on a dilution series of genomic DNA from N. caninum（1.562 5-200 ng/ml）. Brain tissue samples of 32 aborted fetuses were detected by PCR-based assay, and 23 blood samples from mothers were tested by ELISA. Results The amplified DNA fragment （350 bp）had a high identity of 98% with the Nc-5 gene sequence of N.

以环形泰勒虫、牛巴贝斯虫、刚地弓形虫、杜氏利什曼原虫以及犬新孢子虫标准株DNA为模板进行扩增以验证PCR的特异性，采用紫外分光光度计测定犬新孢子虫标准株DNA浓度和纯度，取高纯度的DNA样品用灭菌水稀释，分别取不同量的DNA进行PCR扩增，确定PCR方法的敏感性；利用该方法对32份奶牛流产的胎牛脑组织样品进行检测，同时，对其中23份流产的母牛血样进行ELISA血清学检测，以评价犬新孢子虫PCR方法的检测效果。

The result showed that high quality genomic DNA could be got from both fresh and frozen tissues except scale by common method. Tissue fixed with 70% alcohol and 10% formaldehyde could also extract high quality genomic DNA after effective pretreatment. Noteworthy high quality DNA could be got from scale with fresh and some other fixation methods.

结果表明，除鳞片外，新鲜组织、-20℃冰冻组织以常规方法可提取出较高质量的DNA；保存于70%酒精或10%甲醛溶液中的组织，经预处理，亦可得高质量DNA；新鲜、冰冻、70%酒精或短时间10%甲醛固定的鳞片经适当预处理也可提取出较高质量的DNA。

The mitochondrial genomic sequence in snappers and cobia are similar to other vertebrate mitochondrial genomes with respect to gene order and genomic organization.

线粒体基因组的比对分析表明本文测定的mtDNA基因组的绝大部分区段与GenBank中现有的脊椎动物的序列有较高的同源性。

The foxtail millet genomic sequence, which will be completed soon, shall accelerate the advance of foxtail millet genomic study and improve its academic level.

即将完成的谷子基因组测序，也加速了谷子分子标记和功能基因组研究的进程，提升了研究水平。

To establish a new method to isolate genomic regulatory sequence using unpurified nuclear extract, here a new strategy was developed based on modified whole genome PCR and electrophoresis mobility shift assay. The principle is as follows. After sonication and ligation with DNA linker, human genomic DNA is amplified and labeled with isotope by whole genome PCR.

为了建立一种利用未经纯化的细胞核粗提物就能从人基因组中快速、大量筛选DNA调节片段的新方法，本研究对原有的全基因组PCR技术进行改进，结合凝胶阻滞实验，建立了筛选DNA调节片段的新方法。

With Actinidia which is abundant in secondary metabolites of polysaccharide and polyphenol as materials, the extraction effect of different variety and method on the genomic DNA of Actinidia was compared with, and influence of the different part of tissue culture, antioxidant and the method of DNA purification on the yield of genomic DNA was studied.

方法］以富含多糖和多酚次生代谢产物的猕猴桃为材料，比较不同品种在不同方法下的基因组DNA抽提效果，并对不同材料部位、抗氧化剂以及DNA纯化方法等对基因组DNA的获得进行研究。

After thecomplete genome extraction of the strain was performed, the genomic DNA was partiallydigested by restriction enzyme Sau3AⅠ, the DNA fragments from 1 to 5Kb was clonedinto prokaryote expression vector pET-28a-c, and transformed host bacteria. The resultsshowed that we succeeded in constructing the gene expression library of haemophilusparasuis serovar 5, which is fundamental for the study of advanced gene screening. Inaddition, primer design was performed based on haemophilus influenzae in this study. In addition, PCR was performed by using genomic DNA of haemophilus parasuisserovar 5 as the template. The results demonstrated that we obtained two neo-gene:23SrRNA gene(conserved gene belonging to the large-subunit of ribosome) and adenylatecyclase gene(encodes adenylate cyclase and participates in converting adenyl nucleosidetriphosphate to cyclic adenosine3",5"-monophosphate). Furthermore, the phylogeneticanalyses between the species was performed, and neighbor-joining tree was constructedbased on comparison of 23S rRNA gene sequences, so it was illuminated betweenHaemophilus parasuis and other species in molecular evolution relationship.

选择我国流行优势菌株副猪嗜血杆菌血清5型地方株为研究对象，提取细菌基因组DNA，用限制性内切酶Sau3AⅠ对基因组DNA进行部分酶切，回收大小为1～5Kb的DNA片段，将其连接入原核表达载体pET-28a-c，最后转化宿主菌，结果成功地构建了基因表达文库，为后续的基因筛选工作奠定基础；另外，本研究选择嗜血杆菌属的流感嗜血杆菌为参考对象进行引物的设计，以副猪嗜血杆菌血清5型地方菌株的基因组DNA为模板，进行PCR扩增反应，结果表明成功地获得两个新基因：23S rRNA基因(存在于核糖体大亚基中的保守性基因)和腺苷酸环化酶基因(负责将腺嘌呤核苷三磷酸转变为环腺苷酸)，并进一步做了不同物种之间的分子系统发育分析，构建了基于23S rRNA基因的邻接法系统发育树，阐明了副猪嗜血杆菌与其它菌种的分子进化关系。

The methods of improved CTAB,rapid CTAB,improved SDS and improved low pH medium were used for genomic DNA isolation in Carya cathayensis.The results indicated that the method of improved SDS was the most suitable one for Carya cathayensis genomic DNA isolation and the DNA isolated can be used for PCR reaction.

以山核桃嫩叶为材料，对分别用经过改良的SDS法、改良的CTAB法、简易CTAB法及改良的高盐低pH法提取的基因组DNA进行检测比较，结果显示，改良的SDS法更适合于山核桃基因组DNA的提取，此方法提取的DNA能很好地用于PCR扩增。

Genomic DNA was successfully extracted from fresh leaves of Toona ciliata var. pubescens using CTAB method. Microsatellite DNA was isolated from genomic DNA with improved method of streptavitin beads.

利用改良的链亲和素磁珠法亲和捕捉出毛红椿基因组微卫星DNA片断，并构建了富含微卫星的基因组文库。

The barley chromosome in wheat was identified by genomic in situ hybridization in which biotin labelled total genomic DNA of barley betzes was used as probe and the unlabelled total DNA of common wheat Chinese Spring as blocking DNA. A series of wheat materials were tested as follows; two disomic alien substitution and monosomic alien addition lines, 2n=43; two monosomic alien substitution lines.

用生物素(Biotin-16-dUTP)标记的大麦Betzes基因组DNA作探针，以普通小麦中国春总DNA作封阻进行基因组原位杂交(Genome in situ hybridiz ation，简称GISH)，从13株小麦-大麦杂交后代中鉴定出2个含有3条大麦B etzes2H染色体的材料(2n=43)；2个2H单体异代换系(2n=42)；7个2H二体异代换系(2n=42)。

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克，果实色泽鲜艳，汁多味浓，甜酸适口，离核仁甜，清末时就驰名陕、甘两省，清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异：红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感，ED90值分别为47.65、71.67和29.17g/hm2；春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感，ED90值分别为96.91、114.20g/hm2；而番茄不敏感。