查询词典 genomic

However, the museum C. heterodon specimens were preserved in formalin. It is necessary to approach the methods of effectively extracting the genomic DNA from formalin-fixed C. heterodon, and the feasibility of using the extracted DNA in microsatellite-and mitochondrial analyses. In the present paper, the genomic DNA of ten formalin-fixed C.

然而，过去的铜鱼标本都保存在福尔马林溶液中，有必要探讨从福尔马林固定的铜鱼标本中有效提取基因组DNA的方法以及这些DNA用于微卫星和线粒体分析的可行性。

The comparison between the GuHMGR1 genomic sequence with the cDNA of Pisum sativum showed that the genomic DNA contained two introns.The lengths of three exons were 1206 bp,181 bp and 943 bp,respectively.

GuHMGR1基因在DNA水平上由3个外显子和2个内含子组成；GuHMGR1的cDNA全长为2330bp，其中包括1518bp的开放阅读框、311bp的5'非翻译区和501bp的3'非翻译区。

In this study, the genomic structure and regulatory elements of ApoD from 10 representive species including protozoan, invertebrate, protochordate and vertebrate were analyzed and compared. The genomic structure of ApoD is less conserved in organisms from protostome to deuterostome invertebrates, while it is highly conserved among chordates including amphioxus and verebrates. All four conserved cysteine residues are present in amino acid sequence of deuterostome ApoDs, while there are only two cysteine residues in amino acid sequence of protostomes ApoD. Structure divergence between protostome and deuterostome ApoD proteins suggests their function difference. The majority of regulatory elements are present in nearly all organism ApoD genes ranging from unicellular protozoan to mammals, suggesting that ApoD plays a very fundamental role, and possesses a conserved regulatory mechanism. However, there also exist some specific regulatory elements, which are present only in certain species and may perform some special roles.ApoD mRNA expression in murine NIH/3T3 fibroblasts exposed to various stresses such as as hydrogen peroxide and UV light shows dose-dependent increase. And a fly homolog of ApoD, Glial Lazarillo, whose overexpression results in increased resistance to hyperoxia as well as a extension of lifespan under normoxia and resistance to starvation without altering lipid or protein content.

本文首先从生物信息学角度对分属于原生动物、无脊椎动物、头索动物和脊椎动物类群的10种动物ApoD的基因结构及调控区的调控元件进行分析及比较，发现：(1)ApoD基因外显子-内含子结构从原生动物草履虫到原口动物再到后口动物海胆的进化过程中不保守，但在分析的几种脊椎动物中相当保守；(2)文昌鱼ApoD基因扮演从无脊椎动物到脊椎动物承上启下的角色，可能代表了脊椎动物ApoD基因原型；(3)四个半胱氨酸保守位点在后口动物中都存在，而在原口动物中只存在两个，原口、后口动物ApoD蛋白一级结构上的差异反映蛋白功能上可能也存在一定差别；(4)调控区大多数主要调控元件为不同动物共有，说明ApoD主要功能及其表达调控在进化中相当保守；(5)ApoD基因个别调控元件是随着物种进化而出现并开始发挥相关作用，如SF-1；还有一些调控元件在进化过程中还没有发现其规律，这说明ApoD某些功能和基因表达调控模式可能因物种不同而存在一定的差异。

Since genomic DNA of high quality is a crucial prerequisite for molecular ecology studies, we specially detected efficient protocols to isolate high-quality genomic DNA from ethanol-preserved Pseudaletia separata adults of several natural populations trapped by light traps in fields of different areas.

高质量的基因组DNA样品是分子生态学研究的先决条件。

Currently, by exploiting genomic and associated protein information through in silico analyses, post-genomic research is developing rapidly.

最近，借助基因组信息挖掘技术和蛋白信息的芯片分析技术，后基因组研究正在蓬勃发展。

Currently, by exploiting genomic and associated protein information through in silico analyses, post-genomic research is deeloping rapidly.

最近，借助基因组信息挖掘技术和蛋白信息的芯片分析技术，后基因组研究正在蓬勃发展。

Secondly, the genomic DNA is extracted from Zostera marina L. and then amplified by PCR with two oligonucleotide primers synthesized to obtain the partial nucleotide sequences of the Na~+/H~+ antiporter gene. The Na~+/H~+ antiporter gene clone was obtained after a series of manipulation: purificatioin of PCR product, ligation and transformation of recombinant plasmids, blue-white selection and so on. DNA sequence analysis shows that its DNA sequence is highly homologous with the genomic DNA sequence of E.

其次，通过提取大叶藻基因组DNA，根据设计的简并性引物进行PCR扩增，纯化PCR产物，连接，重组质粒转化，蓝白斑筛选等操作，并测序，提交测定序列到NCBI进行BLASTN，结果表明：该序列与大肠杆菌的基因组序列相似性较高，而与其它物种的Na~+/H~+逆向转运蛋白基因没有相似性。

The genomic DNAs of the transgenic plants carrying Ac:: GUS were probed by Ac DNA in Southern blot analysis. Ac had excised from its original positions in T-DNA and reinserted at other genomic sites.

Ac因子作探针，与上述转基因植株的基因组DNA进行Southern杂交，结果表明，Ac从T-DNA解离后，重新整合到烟草基因组的其它位点。

The research into ways of extraction of genomic DNA of Jinhua pear indicated that improved CTAB was the best way to extract genomic DNA of pears, with the dose of 0.10g/g of Vc added when the sample is triturated,.

2经金花梨基因组DNA提取方法研究表明，磨样时在鲜样中添加0.10g／g的Vc而进行改良的CTAB法是提取梨DNA的最佳方法，嫩芽是最佳提取材料，其次是新梢和成熟叶片。

Its chromosome number was 42, and 21 bivalents could be observed at PMC MI. FISH analysis by using genomic DNAs from S. cereale and genomic DNAs from L.

染色体观察结果表明，山农030-1根尖染色体数目为42，花粉母细胞减数分裂中期I染色体构型为2n=21Ⅱ。

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克，果实色泽鲜艳，汁多味浓，甜酸适口，离核仁甜，清末时就驰名陕、甘两省，清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异：红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感，ED90值分别为47.65、71.67和29.17g/hm2；春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感，ED90值分别为96.91、114.20g/hm2；而番茄不敏感。