查询词典 genes

This study was designed to clone the duck FABP gene based on the sequences of chicken and mammal FABP gene by comparative genome and bioinformatics approaches; to investigate the expression characterization of FABP genes by RT-PCR; to detect the polymorphisms of FABP genes by DNA sequencing, PCR-SSCP methods, and to investigate the effects of FABP genes polymorphisms on growth and body composition traits in Kunshan sheldrake, Cherry Valley Meat duck and so on.

本研究以鸡、哺乳动物FABP基因序列为基础，通过比较基因组学和生物信息学等方法克隆鸭的FABP基因；采用RT-PCR方法研究FABP基因的组织表达特性。

Results (1) The forward and reverse subtracted cDNA libraries of different metastastic potential large cell lung cancer cell lines were successfully constructed;(2) With blue-white colony and dot blot, 307 positive clones in the forward subtracted library and 78 positive clones in the reverse subtracted library were obtained;(3) 55 clones were successfully sequenced in the forward subtracted library. Homolog analysis confirmed 23 differential expression segments, which were similar to the human genes already known, including NME2, NPM1, MT 2A, HSPE1, TAFIA, EPRS, PX19 and EIF3S9 et al;(4) 31 clones were successfully sequenced in the reverse subtracted library. Homolog analysis confirmed 16 differentially expressed segments. 15 of them were similar to the human genes already known, including ANXA2, TUBB, PKN2, GNAS, EEF1A1, SSR2 and RPLPO et al. Only one segment had partial homology to known human genes. This segment was supposed to be the new EST segments which might not have been cloned.

结果(1)成功构建人大细胞肺癌高低转移株差异表达基因正向消减cDNA文库和反向消减cDNA文库；(2)经蓝白菌落筛选和斑点杂交，正向消减文库获得307个阳性克隆，反向消减文库获得78个阳性克隆；(3)正向消减文库挑选55个克隆成功测序，经同源性分析最终确定差异表达基因片段23个与已知人类全长基因具有很高相似性(95％～100％)，这些基因包括NME2、NPM1、MT2A、HSPE1、TAFlA、EPRS、PX19和EIF3S9等；(4)反向消减文库挑选31个克隆成功测序，经同源性检索和比对最终确定差异表达基因片段16个，其中15个与人类全长基因具有很高相似性(95％～100％)，包括ANXA2、TUBB、PKN2、GNAS、EEF1A1、SSR2和RPLPO等基因。1个片段和己知人类基因仅有部分相似性，表明它可能是未被克隆的人类基因EST片段。

Japonicum, covering 90% of its total genes; find 8400 protein-coding genes; set up the largest functional gene database and clone libraries in the world; for the first time, identify as many as 3260 proteins from variety of life stages of S. japonicum; analyse the eggshell proteins and tegument proteins, which were the putative targets for drugs and candidates of vaccines; reveal a large quantity of genetic polymorphisms in the genes of S.

对人CD34+造血干／祖细胞进行了转录组和蛋白质组学研究，鉴定了370个蛋白质，这是迄今为止鉴定最多的CD34+细胞蛋白，并且鉴定了各种转录组研究没能发现的133个CD34+细胞蛋白，发现了CD34+细胞具有可塑性的新的证据，证明了反义核酸未能完全阻止其相应基因的表达，并提出一些蛋白的异质性是由于翻译后修饰造成；2。

Using semi-quantitative RT-PCR, the differential expression profiles of eleven selected genes were confirmed in the ovaries of triploid and diploid. These genes fell in gene categories with a wide range of functions. The results indicated that triploidy affects the dynamic gene regulatory network in triploid ovary. This study established a firm basis for future investigation on characterization of crucial molecular events for normal ovarian development in shrimp.To further dissect exact gene functions for gonad development of shrimp, three differentially expressed genes between diploid and triploid ovary, PCNA (proliferating cell nuclear antigen), CAS/CSE1 (cellular apoptosis susceptibility protein/chromosome segregation 1) and SSRF (spermatogonial stem-cell renewal factor) were characterized on certain aspects.

利用抑制性消减杂交技术，建立了对虾二倍体和三倍体卵巢间的2个消减文库；在正向消减文库(以三倍体卵巢作为实验组，二倍体卵巢作为驱动组)中，鉴定到54个基因；在反向消减文库(以二倍体卵巢为实验组，三倍体卵巢为驱动组)中，鉴定到16个基因；选取11个差异表达的基因，利用半定量RT-PCR的方法对其在二倍体和三倍体卵巢间的表达进行了检测，均能很好地与消减结果相吻合；这些差异基因编码多种功能的蛋白，分析表明染色体的三倍化使三倍体卵巢中的基因调控网络受到了影响；为深入揭示维持卵巢正常发育的关键分子调控事件奠定了基础。

The comparison of the genes with white-tailed deer and red deer PrP gene revealed that the nucleotide and its putative amino acid identities were 97.4%-98.2% and 97.7%-98.8%, respectively, it show the PrP genes of ruminant among the same family or different families are all conservative. The comparison of the genes with white-tailed deer and red deer PrP gene revealed there are five base substitutions produced amino acid mutation, e.g.

与同科不同亚科的马鹿和白尾鹿以及不同科的奶牛和绵羊朊蛋白基因序列比较，核苷酸序列和推导氨基酸序列同源性为97.3%～98.2%和97.7%～98.8%，说明朊蛋白基因在同科和不同科的反刍动物间是保守的。

Comparison of genes in yolk sac cells with a total of 1 200 genes in the control cells, 79 genes differently expressed between the two groups were detected.

在神经管缺陷大鼠胚胎卵黄囊细胞和对照组1 200个基因中，共筛选出表达差异基因79个，其中42个基因表达上调、37个基因表达下调。

Firstly, the SSH was used to isolate tissues or organs specific expression genes in different development stages of plant. These genes provide evidence for explaining the molecular mechanism of plant development. Then SSH was widely used to isolate genes related to resistance under biological or abiological stress in different plants and the molecular mechanism of the resistance was primarily elucidated.

首先， 官中的组织特异性表达的基因，为揭示植物生长发育过程的分子机理提供了有效手段；另外，在不同植物中，该技术被大量应用于分离各种生物及非生物胁迫条件下诱导表达的抗性相关基因，可以揭示植物抗逆的分子 SSH 技术已开始应用于分离与次生代谢产物合成相关的基因。

We also summarized the genes which expressed 5-fold (116 genes) and 10-fold (32 genes) higher in the amoeboid stage.

我们也选取了在阿米巴体时期基因表现量大於5倍(116个基因)及10倍（32个基因）的基因做分析。

On comparing with the common type 4, there are 18 genes presenting change in the rest 21 types, most of them are tRNA genes, and gene replacement, gene increase and gene absence are frequently happened; on the contrary, protein genes are more steady, gene change is mostly of gene replacement. The genome size of Gymnophiona are all smaller than 18000 bps and most of them range from 15000 to 16000 bps, those of Urodela and Anura are bigger than 16000 bps, most of Urodela range from 16000 to 17000 bps, most of Anura range from 17000 to 18000 bps.

与类型4比较，其余21种线粒体基因组类型涉及基因变动的基因共有18个，其中变动比较多的是tRNA基因，移位、增多和缺失的发生频率都较大，而蛋白编码基因比较稳定，主要是移位。78种两栖动物中，蚓螈目的线粒体基因组均小于18000bps，多数在15000~16000bps；有尾目和无尾目均大于16000bps，其中有尾目多数在16000~17000bps，无尾目的多数在17000~18000bps。

We summarized the progress in research on avirulence genes of the rice blast fungus, including the importance of the research, avirulence genes cloned and their interaction characteristics with rice resistance genes.

本文从研究稻瘟菌无毒基因的意义、已鉴定和克隆的稻瘟菌无毒基因、稻瘟菌无毒基因与其抗病基因的互作特点等几个方面，对稻瘟菌无毒基因研究进展作了简要评述。

Chrysanthemum of 10 thousand birthday is lax to edaphic requirement, with the arenaceous qualitative loam with fecund, good drainage had better.

万寿菊对土壤要求不严，以肥沃。排水良好的砂质壤土为好。

He unstepped the mast and furled the sail and tied it.

他拔下桅杆，把帆卷起，系住。