查询词典 genes

Therefore, the LsHNXs genes were cloned in this study, and their function was studied by RNAi. The main results were shown as follows:1 The conservative sequences of three LsHNXs genes (LsHNX1, LsHNX2, and LsHNX3), and the 3'-terminal specific sequences of LsHNX2 and LsHNX3, were cloned by RT-PCR and 3'-RACE.2 The plant RNAi vectors were constructed and the transformation of Limonium sinense was done. The RNAi lines of three single-genes and one double-gene were obtained and were propogated rapidly for the molecular and the stress tolerance analysis.3 To be compared with wild type, the Na+ and K+ contents secreted by the leaves of LsNHX1 transgenic plants were remarkably higher.

主要实验结果如下：1采用RT-PCR和3'-RACE方法从补血草中克隆获得LsNHXs基因家族三个成员LsNHX1、LsNHX2和LsNHX3的保守区序列以及LsNHX2和LsNHX3的3'端特异序列。2构建植物基因RNAi沉默表达载体并转化补血草，获得三个单基因和一个双基因的RNAi沉默表达植株，快繁转基因植株供分子鉴定和耐逆分析。3LsNHX1转基因植株叶片表面分泌物中的Na+、K+离子含量比野生型对照显著增加；叶组织内Na+离子含量和K+离子含量均略高，但差异不显著；K+/Na+比没有显著差异。4LsNHX2转基因植株叶片表面分泌物中的Na+离子含量比野生型对照有所下降，但差异不显著；K+离子含量比对照显著降低。

Compared the sequences of rRNA genes in A. pernyi with that of other insect mtDNA, the 16SrRNA genes are longer except for B. mori and B. mandarina, and equal to A. mellifera; the 12S rRNA genes are shorter except for T. imagines, and equal to H. macropus.

柞蚕线粒体DNA编码的16SrRNA较长，比家蚕和野桑蚕的小，但仅差几个bp，与蜜蜂相近，而长于其他所比较昆虫。12SrRNA则较短，仅长于蓟马，与咬虱相近，而短于其他昆虫。

The Major histocompatibility complex of Macaca mulatta, also called Mamu antigen, is a gene cluster directly related to immune response. Evolution creates the highly polymorphic nature of the MHC genes, and this leads to changes in the number as well as the function of these genes in individual Macaca mulatto, and even the emergence of species-specific genes.

恒河猴是应用最广泛的非人灵长类实验动物，其MHC基因是一个庞大的与免疫功能密切相关的基因群，在进化过程形成了Mamu基因不同的存在状态，使得不同个体的Mamu基因在数量和功能上有所差异，同时有些个体还产生了特异性的MHC基因，它的多态性和免疫反应的复杂性相对应。

Moutains of evidences from laboratary and clinical data show that gene abnormality is the major etiological factor of over 40% patients affected with epilepsy.There were also 6 epilepsy genes been cloned successfully, and mutations of over 1000 genes maybe related to epilepsy. These abnormal genes influence many aspects from molecular level to the neuronal plasticity, such as the development of brain,neuronic degeneration, remodeling of neural circuits, energy metabolism, ion channel and so on.These factors would at last affect the formation of epilepsy focus, propagations of epileptic potentials, vulnerability of epilepsy cells.

大量的实验和临床资料提示基因异常是40%以上癫痫患者的病因，有6种常见全身癫痫的基因已被成功克隆，1000种以上的基因突变可能与癫痫的发作有关，这些表达异常的基因分布在脑发育、神经元变性及神经环路重组、能量代谢、离子通道等多个环节，从分子、细胞、神经元可塑性等多个方面影响着癫痫灶的形成、痫性放电的扩布及癫痫细胞的损伤，构成了癫痫的基因机制。

The resulting strain, designated YES2MTM1, was transformed with a yeast genomic library. Transformants lost the plasmid overexpressing MTM1 after 5-FOA treatment. Yeast strains able to grow on nonfermentable carbon source with MTM1 deletion and overexpression of some DNA fragments were picked up and candidate suppressor genes were identified. Overexpression of five genes were identified to be able to rescue the growth defect on nonfermentable carbon source. The study will provide reference for MTM1 gene function and screening for suppressor of genes whose deletion result in irreversible damage.

为了避免MTM1缺失造成的不可逆损伤，在野生型酵母中先转入带有MTM1 基因的质粒，再敲除染色体上的MTM1 基因，随后转入基因组文库，再利用药物5-氟乳清酸(5-FOA)迫使细胞丢失表达MTM1基因的外源质粒，再筛选能在非发酵培养基上生长的转化子，通过这种方法筛选发现，POR2等5个基因的过表达可以挽救MTM1 基因缺失造成的非发酵培养基上的生长缺陷，为深入了解MTM1基因的功能提供了线索，对筛选其他造成不可逆损伤的突变基因的抑制基因提供了一条可行的研究思路。

The discussed emphasis was put on the expression profile of similarity to rice MADS box genes, phylogenic kin of new genes and some function-known genes.

其中既有与雄蕊有关的，又有与雌蕊和胚胎发生有关的，还有与雌雄蕊都有关的基因。

It is estimated that there are about 40,000 functional genes.In order to understand those genes related to human diseases and the novel functional genes, the genetic manipulated mice including transgenic mice and knockout mice have been generated to facilitate the purpose.This transgenic core has been collaborated with industrial and research institutes for years, and has built up competitive know-how for transgenic mice, embryo cryopreservation, and pathogen free rederivation.

小鼠长期以来即为研究人类疾病最佳动物模式，2001年美国赛洛因公司已将小鼠129的两个品系之基因序列解码完成提供付费使用，2003年小鼠B6的基因序列也被Mouse Genomic DNA Sequencing Consortium解码完成，预估约有4万个功能基因，随著基因操控技术的成熟，制造出各种带不同基因缺陷的模式动物供生物医学研究已非难事。

This report identified S-RNase genes of sweet cherry (Prunus avium L.), presented the sequences of S genes by using a pair of specific primers PruC2 and PruC4R based on the conserved regions C2 and RC4 of Rosaceous S-RNase genes and researched the S gene specific productsfrom the genomic DNAs of different cultivars in which most of the S genotypese were unknown.

根据蔷薇科S-RNase基因高度保守区C2 和RC4区设计一对特异引物PruC2 和PruC4R，对甜樱桃品种的基因组DNA进行S基因特异PCR扩增。

In order to understand the expressed genes, construct the known gene expressed profile, study the tissue-specific genes and the common genes from pig and chicken; In order to do primary researches for comparative genomics, functional genomics, QTLs mapping, animal molecular breeding and so on, we have constructed 3 cDNA libraries of Xiang Pig muscle tissue, Silkie Fowl muscle tissue and liver tissue, sequenced a large scale ESTs and analyzed their bioinformation from the ESTs of Silkie Fowl muscle tissue and liver tissue in our study.

为全面了解肌肉组织和肝脏组织的基因表达类型，构建猪、鸡肌肉组织和肝脏组织的功能基因表达谱，研究肌肉组织和肝脏组织的特异表达基因及这两种组织的共同基因；为比较基因组学的研究、功能基因组学的研究、QTLs定位等的动物分子育种做前期基础科研工作，本研究构建了香猪肌肉组织、丝毛乌骨鸡肌肉组织和肝脏组织的3个cDNA文厍，并对丝毛乌骨鸡的肌肉组织和肝脏组织进行了大规模的ESTs测序及其生物信息学的初步研究。

The article focuses on the aberrant expression and significances of proto-oncogenes, tumor suppressor genes and genes associated with the proliferation process of gastric cells such as telomerase, transcription factor and cyclooxygenase, etc. These genes, alone or synergically, participate in gastric cancer cell proliferation, resulting in an uncontrolled growth characterized by over-proliferation of decreased apoptosis of gastric cancer cells.

本文着重阐述了与胃癌细胞增殖过程密切相关的端粒酶、核转录因子、环氧化酶等基因以及参与细胞周期调控的原癌基因、抑癌基因的异常表达及临床意义，这些基因或单独作用，或相互协同，参与胃癌细胞的增殖，使肿瘤细胞生长以增殖过多、凋亡过少的失控性生长为特征。

Chrysanthemum of 10 thousand birthday is lax to edaphic requirement, with the arenaceous qualitative loam with fecund, good drainage had better.

万寿菊对土壤要求不严，以肥沃。排水良好的砂质壤土为好。

He unstepped the mast and furled the sail and tied it.

他拔下桅杆，把帆卷起，系住。