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Methods The method of PCR- RFLP was conducted to examine the genotype of 3 cPLA2 genes ( PLA2G4A, PLA2G4B and PLA2G4C genes) in 263 subjects, including 121 cases of T2DM and 142 controls. The conditional test was used to test the combined effect of distinct loci on the T2DM by conditioning on allele or by conditioning on genotype with UNPHASED analysis platform. Results The genotypic frequencies of the 3 genes did not deviate from Hardy-weinberg equilibrium in both case and control groups.

采用聚合酶链反应-限制性内切酶片段长度多态性方法检测T2DM患者(病例组121例)和健康对照(对照组142例)3个cPLA2基因(PLA2G4A、PLA2G4B和PLA2G4C基因)的基因型;借助UNPHASED分析平台,利用等位基因条件分析和基因型条件分析方法分析基因的联合作用与T2DM的遗传相关性。

Breeders rely on these pairing-control genes to ensure that soybean varieties carrying the technology include the full suite of resistance genes as well as genes that lead to higher yields, said Rick Vierling, adjunct professor of agronomy and director of the genetics program at the Indiana Crop Improvement Association.

印第安那州作物改良协会农业经济副教授、基因项目主任里克·维尔林说,育种公司依靠这种配对控制基因可以确保基于该技术的大豆品种含有全部抗病基因及高产基因。

The results showed that the expression of calm3 and trm112l genes had no remarkable differences in oocytes and 8-cells stage embryos, but were significantly different in blastocysts. It was supposed that these two genes were possibly maternal genes, and they might be involved in oocyte maturation and zygotic genome activation.

结果显示,在卵母细胞和8细胞期胚胎,基因calm3和trm112l的表达量无显著性差异,而在囊胚期的表达量与前两个时期的表达量存在显著性差异,推测这两个基因在牛胚胎发育过程中的表达可能属于母型调控,提示该基因在卵母细胞成熟和合子基因激活中起一定作用。

Therefore, an expansion of the inheritance model, such as linkage between two major genes, three major genes, three major genes plus polygenes, was developed in this paper.

在文献 1~ 4的基础上,拓展利用亲本和DH或RIL群体的 2对连锁主基因、2对连锁主基因+多基因、3对主基因和3对主基因+多基因 4类遗传模型。

Then the plants were used to be crossed with parental lines of two-line hybrid rice, 9311, E32, Peiai 64S and W9834S etc. Seventeen BC,F, plants carrying genes Xa23, Pi-b and IPT genes pyramided, and 6 BC2F plants with Xa23 and IPT genes pyramided were detected.

并通过回交向两系亲本9311、E32、培矮64S和W9834S转移,最终获得了17株抗衰老、抗白叶枯病和抗稻瘟病三基因聚合BC_1F_1植株及7株IPT基因与Xa23基因聚合BC_2F_1植株。

Therefore it is interesting to clone genes related to lemma and palea. In this thesis, six genes from the lemma/palea related gene pool which is established by Liu (2003) were further studied. Those genes are salT gene (salt-induced protein), GA-SPY gene (gibberellin action negative regulator SPY-related protein), U2AF gene (U2 snRNP auxiliary factor, small subunit-related protein), kinesin-like gene, DnaJ-like gene, and EF hand gene. At first, the gene expression in leaves and 1~4 cm inflorescences of normal lemma/palea, smaller lemma/palea, and stunted lemma/palea was compared by Real Time RT-PCR.

从刘(2003)所建立水稻颖花发育相关候选基因库中,挑选6个基因:salT基因(salt-induced protein)、GA-SPY基因(gibberellin action negative regulator SPY-related protein)、U2AF基因(U2 snRNP auxiliary factor,small subunit-related protein)、kinesin-like基因、DnaJ-like基因、及EF hand基因,以水稻正常型颖花、小颖花突变体及内外颖退化突变体之叶片及1~4公分幼花序为材料,利用Real-Time RT-PCR分析,发现salT基因在突变体幼花序中大量表现。

Selecting the better amplified from Diacylglycerol acyltransferase, gene, Acyl- desaturase gene ,then cloning sequencing, afer that, through the NCBI and nucleotide sequence to match homology , the results show that: cloning by the cassava, castor-oil plant Jatropha curcas DGAT and SAD genes and gene fragments have the high homology with other known plant DGAT genes and SAD genes .

从中筛选出扩增效果较好的二酰基甘油酰基转移酶(Diacylglycerol acyltransferase, DGAT)基因,硬脂酰-酰基载体蛋白脱饱和酶(Acyl- desaturase, SAD)基因克隆测序,经测序,通过NCBI与已知的核苷酸序列进行同源性比对,结果表明:克隆所获得的木薯、蓖麻和麻疯树 DGAT基因和SAD基因的片段与其它已知植物的DGAT基因和SAD基因具有很高的同源性。

Selecting the better amplified from Diacylglycerol acyltransferase, gene, Acyl- desaturase gene ,then cloning sequencing, afer that, through the NCBI and nucleotide sequence to match homology , the results show that: cloning by the cassava, castor-oil plant Jatropha curcas DGAT and SAD genes and gene fragments have the high homology with other known plant DGAT genes and SAD genes .

从中筛选出扩增效果较好的二基甘油基转移(Diacylglycerol acyltransferase, DGAT)基因,硬脂-基载体蛋白脱饱和(Acyl- desaturase, SAD)基因克隆测序,经测序,通过NCBI与已知的核酸序列进行同源性比对,结果表明:克隆所获得的木薯、蓖麻和麻疯树 DGAT基因和SAD基因的片段与其它已知植物的DGAT基因和SAD基因具有很高的同源性。

Identification of susceptibility genes for complex diseases in animal model, here rat arthritis as an example, may include the following steps:①selecting inbred animal strains and establishing disease models;② segregating breeding in F2 animals and running linkage analysis to find QTLs;③ producing a series of congenic strains to narrow down QTL region under help of STR or SNPs markers;④ carrying out positional cloning of susceptibility genes, and ⑤ confirming the function of susceptibility genes.

以大鼠类风湿关节炎模型为例,定位克隆易感基因步骤包括:①选择亲代近交系,建立关节炎模型;②分离育种,连锁分析、确定数量性状位点;③建立Congenic系、窄化QTL区域;④位置克隆基因;⑤易感基因的功能验证。

Different contribution of the genes to starch biosynthesisLeaves and developing endosperms of Q319, C7-2, q404, ZN-A and S8 were used to study the expression patterns of 44 genes participating in starch synthesis. We found that ZmGBSSⅠ、ZmSSⅢ、ZmSBEⅡb、ZmBT2-2、ZmSh2-2、ZmSh2-3、ZmBT1 were expressed exclusively in the metaphase and anaphase of developing endosperm, and the expression patterns of ZmSUS1、ZmSus1L、ZmSUS3、ZmSSⅠ、ZmSSⅡa、ZmSBEⅠ、ZmISO1、ZmPul were consistent with the process of starch synthesis in the endosperm, while ZmUGP3 and ZmSUT2 were highly-expressed in all samples, suggesting that these genes perhaps devoted to starch synthesis in endosperm.

淀粉生物合成基因的不同贡献以Q319、C7-2、q404、ZN-A和S8自交系的幼叶、成熟叶、成熟子房、授粉后1d、5d、10d的种子和15d、20d、25d的胚乳为材料,研究了参与淀粉合成的44个基因的表达模式,研究发现ZmGBSSⅠ、ZmSSⅢ、ZmSBEⅡb、ZmBT2-2、ZmSh2-2、ZmSh2-3、ZmBT1在胚乳发育中后期特异性表达,ZmSUS1、ZmSus1L、ZmSUS3、ZmSSⅠ、ZmSSⅡa、ZmSBEⅠ、ZmISO1、ZmPul的表达在淀粉合成期显著上调,ZmUGP3和ZmSUT2在所有组织中高水平表达,说明这些基因可能主要负责胚乳淀粉的生物合成。

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