查询词典 genes

The mechanism of PPS accelerating IEC-6 cells migration was also researched with gene-chip technique. The Altas Clontech Rat cDNA Chip which consisted of 1176 spots representing different functional categories such as heat shock/stress, cell cycle, transcription factors, hormones, metabolism, etc was used. Result showed in cell migration model of IEC-6 cell line, compared with control group, expression of 38 genes in total 1176 genes varied in cells treated by PPS, including 21 genes up-regulated and 17 genes down-regulated. Date analyze showed the mechanism of PPS accelerating IEC-6 cells migration mainly related with promoting the numbers and activity of cellular ion channel, especially 〓 channel.

从以上实验结果中我们可以得出以下结论：四君子汤总多糖具有促进IEC-6细胞增殖、迁移、粘附的作用；四君子汤总多糖无论是对IEC-6细胞增殖、或者迁移的作用均优于各单味药多糖，可能是各种单味药多糖综合作用的结果；多糖可能是四君子汤"益气健脾"药效的主要成分；小肠上皮细胞可能是四君子汤总多糖的作用靶点之一，提示临床采用四君子汤及"益气健脾"法治疗"脾虚证"的疗效机制可能与增强机体的整体免疫和肠道粘膜免疫功能有关。

It is clear from the comparison of the number of AmphiL34 and AmphiS29 genes with that of rat L34 and S29 genes that duplication of L34 and S29 genes occurred in vertebrates like rat. It therefore appears that the housekeeping ribosomal proteins genes such as L34 and S29 also underwent large-scale duplication during the early chordate evolution.

这一结果分别与大鼠7-9个L34拷贝数和14-17个S29拷贝数相比较，证明持家核糖体蛋白基因L34和S29基因在脊椎动物起源与进化过程中发生了大规模扩增，符合两轮基因倍增规律。

In this article, the domain structure of LBD genes, classes of LBD genes in model plants of monocotyledon or dicot, expression model, mutation phenotypes from loss of function or gain of function, and its relationship to other gene families were reviewed. Furthermore, the redundancy character of LBD genes, basic candidate function shared by LOB domain, and potential molecular mechanism of LBD genes interacting with other genes also were discussed.

本文对LBD基因的结构域特征、在单／双子叶模式植物中的分类、表达特点、己克隆LBD基因的功能及与其它基因或基因家族间的相互作用关系进行了综述，并对LBD基因在高等植物中的功能冗余特性、LOB结构域可能所具有的基本功能和LBD基因与其它基因相互作用的分子机制进行了探讨。

The common features of imprinted genes are, clustering of multiple imprinted genes in one chromosomal region (around 80%), conservation of imprinting among eutherian mammals, asynchrony of DNA replication of imprinted genes, temporal and spatial regulation of expression of imprinted genes, coding for untranslated RNAs as well as proteins, antisense transcripts may regulate expression of imprinted genes. Once established, the imprinting pattern is stably transmitted through cell division but reset in germ cells of the fetal gonads.

约80%的印记基因呈串出现在染色体上；在哺乳动物品种之间，印记基因具有较高的保守性；印记基因的复制通常表现为不同时性；一些印记基因具有印记遗传的时空性；少数印记基因只转录为mRNA而不翻译成蛋白质；印记基因的反意链通常表达，表达产生具有调节印记基因的作用。

In animals, mitochondrial DNA is generally a 15-22 kb circular genome containing 37 genes: 13 protein-coding genes, two rRNA genes, and 22 tRNA genes, as well as a control region. All 37 genes are arranged in the same relative order almost in all vertebrate species from teleost fishes to eutherian mammals. In recent years, mtDNA has been widely used as a useful marker system in numerous phylogenetic analyses in vertebrate relationships.

脊椎动物线粒体DNA是一个环形结构，长度约为15-22kb.mtDNA呈母系遗传，具有相对保守性，已被广泛应用于分析动物个体鉴别和动物类群间的系统发生关系。

Using gene chips to screen an SSH library could efficiently identify those differentially expressed genes without the need to examine previously cloned genes, and increase the number of target genes by decreasing the examination of multiple'house-keeping'genes.

因此，基于SSH方法和基因芯片技术的特点，将两者联合应用将是有针对性地发现大量差异表达基因的有效方法，而且可以大大提高发现新基因的机率。

The gene expression were obvious difference between lung cancer tissues and the para-neoplastic tissues,36 genes differential expression of lung squamous cell carcinoma were screened out,comprising 23 known genes, 13 unknown genes, among these genes, up and down regulated genes were 22 and Irrespectively.

Northern blot分析结果：对7条目的基因进行Northern blot分析后，我们首次发现金属硫蛋白一3基因(MT一3)魔在肺腺癌中明显高表达，MT一3在肺腺癌中的表达较在正常肺组织中的表达高5倍以上。

These genes are related to many plant functions, indicating that the heterosis was related with many metabolization pathways.1.2 The quantity of discrepant genes showed that the genetic discrepancy was notalways the same with the discrepancy of expressed genes, indicating that small-scale genetic discrepancy maybe result in large-scale genes discrepantly expression; in other way, some genetic discrepancies might not embodied on the discrepant genes in the experiment.1.3 Nine and eight discrepant genes were found respectively in the superior bulkand minor bulk in the dominant level.

1.2 各张芯片差异表达基因数量表明遗传差异与表达谱差异不尽一致，可能较小的遗传差异可以造成表达谱的巨大改变，而有些遗传变异可能没有在表达谱上表现出来。 1.3 从优势集团和负优势集团中分别找到9个和8个具显性表达水平的差异表达基因，研究表明显性表达水平的差异表达基因的聚合与杂种优势的表现没有相关性。

We selected mecA gene and femA factor asβ-lactam antibiotic-resistant genes, ermA, ermC, msrAgene as macrolides-lincosamids antibiotic-resistant genes, norA, grlA, gyrA, gene as Quinolonesantibiotic-resistant genes, and tetM gene as Tetracycline antibiotic-resistant genes on the basis of the latestresearch. We designed primers with GenBank enunciable gene sequence, cloned the drug resistantgenes, and sequenced these genes.

根据国内外最新文献报道，筛选出对β-内酰胺类抗生素耐药的mecA基因及辅助因子femA；对大环内酯类及林可霉素类抗生素耐药的ermA、ermC、msrA基因，对喹诺酮类抗菌药物耐药的norA、grlA和gyrA基因及对四环素类抗生素耐药的tetM基因作为拟选的主要耐药基因，分别设计PCR扩增的特异性引物，进行克隆。

Drug sensitive test and three-dimensional test220 strains of Pa were isolated from hospitalized patients between 2003 and 2007. K-B method was used to tested the susceptibility of 10 different antibiotics. IRPa was screened by testing the minimal inhibitory concentration of imipemem by using agar diluiion method.The susceptibility of these IRPa to the antibiotics was analysised. Three-dimensional test was used to identify the different kinds of beta lactamases from 220 strains of Pa.2.Carbarpenems hydrolytic enzyme genes and oprD2 gene were detectedamong the selected IRPa strains, PCR method was performed to detect carbapenemase genes which included GES、KPC、SPM、VIM、IMP、GIM gene and the oprD2 gene;Multiplex PCR were used to detect OXA genes and plasmid-mediated AmpC beta lactamase genes; The expression of the chromosomal AmpC beta lactamases and oprD2 genes in IRPa strains were analyzed by Real-time PCR.3.Identification and characterization of integronsIntegrase gene was detected by PCR, and the classification of integrons was performed by using restriction fragment length polymorphism.PCR was performed to detect the qacE△1-sull gene,and the gene cassetes which are located at variable region of integrons in the strains were detected to be positive.

方法1、药敏实验和三维实验收集2003～2007年临床分离的220株Pa，对这些菌株采用K-B法测定10种临床常用抗生素的药敏情况，同时采用琼脂稀释法检测亚胺培南的最低抑菌浓度(Minimal inhibitory concentration，MIC)，筛选出对亚胺培南耐药的铜绿假单胞菌，并分析其对其它抗生素的药物敏感率；采用三维实验的方法分析220株Pa产β内酰胺酶的类型。2、碳青霉烯类水解酶和oprD2蛋白的检测针对鉴定的IRPa菌株，采用普通PCR方法检测具有碳青霉烯水解作用的β内酰胺酶耐药基因(GES、KPC、SPM、VIM、IMP、GIM基因)和oprD2基因，采用多重PCR的方法检测OXA型基因和质粒携带的AmpC酶基因，用荧光定量RT-PCR方法检测oprD2蛋白基因表达情况；同时对产AmpC酶的Pa(25株，含IMP耐药和敏感株)用RT-PCR方法检测AmpC酶基因的表达量情况。3。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。