查询词典 fluorescence

The direct interaction of copper ion with CuZnSOD was studied by fluorescence spectroscopy, and a type of 1:1 stable complex has been generated, which was certified by the quenching experiment of intrinsic fluorescence. The process of the generated complex shown by dynamic analysis of fluorescence quenching, consisted of two steps, in which the first step was a quick association process of double molecules to generate a loose complex, and the second step was a slow process of single molecule, where the loose complex was isomerized into the compact complex.

通过荧光光谱方法研究了这种相互作用，内源荧光的猝灭实验表明Cu(上标 2+)与CuZnSOD形成1：1型稳定配合物；荧光猝灭的动力学分析表明配合物形成过程由两个独立步骤完成：第一步是双分子快速缔合过程，形成了结合疏松的配合物，第二步是单分子慢速过程，即松散的配合物&异构化&成为结合紧密的配合物。

The direct interaction of copper ion with CuZnSOD was studied by fluorescence spectroscopy, and a type of 1∶1 stable complex has been generated, which was certified by the quenching experiment of intrinsic fluorescence. The process of the generated complex shown by dynamic analysis of fluorescence quenching, consisted of two steps, in which the first step was a quick association process of double molecules to generate a loose complex, and the second step was a slow process of single molecule, where the loose complex was isomerized into the compact complex.

通过荧光光谱方法研究了这种相互作用，内源荧光的猝灭实验表明Cu2＋与CuZnSOD形成1∶1型稳定配合物；荧光猝灭的动力学分析表明配合物形成过程由两个独立步骤完成：第一步是双分子快速缔合过程，形成了结合疏松的配合物，第二步是单分子慢速过程，即松散的配合物&异构化&成为结合紧密的配合物。

The result showed that the maximum chlorophyll index was more suitable for the estimation of chlorophylla concentration in the water body of Taihu Lake than the fluorescence line height in the inversion analysis for the estimation of chlorophylla concentration using MERIS fluorescence parameters, and the normalized fluorescence height (NLHR681/R665) had the best fit with the measured chlorophylla concentration.

结果表明：MERIS荧光参数中最大叶绿素指数较基线荧光高度更适合太湖水体叶绿素a浓度的反演；归一化荧光高度与实测叶绿素a浓度间的拟合效果最好。

Methods pna conjugated fish (fluorescence in situ hybridization)-fitc was performed on paraffin sections from melanoma,melanoma metastases and common naevi, respectively. telomere mean maximum diameter, area and number per nuclear area were calculated by image analysis on fluorescence images elaborated through confocal laser scanning microscope and fluorescence microscope.

应用肽核苷酸探针标记荧光原位杂交—荧光素异硫氰酸法检测黑色素瘤、转移黑色素瘤及黑色素痣石蜡切片中的端粒，以荧光显微镜、激光共聚焦显微镜生成图像，并详细记录细胞核平均面积、端粒数目、端粒面积。

And 17 were capable of detecting specific metal ions by showing dramatic enhancement of fluorescence. In contrast with 15 and 17, the fluorescence signals of 13 and 14 were inefficiently altered by metal ions. Hence, 15 and 17 were further investigated with detailed 1H-NMR, UV-vis, and fluorescence titrations.

其中，15和17对特定金属离子有明显的侦测能力，混合后萤光强度会大幅增强，但13及14与金属离子混合后，萤光讯号的改变幅度不如15及17的显著，因此我们只对15和17进行详细的1H-NMR、UV-vis及萤光光谱的滴定。

Through this topic, we discover with the 2%NaOH-NALC digesting fluid may in 10 minute in rapid decomposition sputamentum resisting medium, during guarantee digestion effect not because the NaOH strong function will have killed the bacterium (timing control in 20 minutes), also will not affect the magnetic pellet the function; Through the application self-restraint quota simulation clinical specimen, we have compared the acid-fast stain and the pyoktanin yellow O fluorescence dye pick out limit, the pyoktanin yellow O fluorescence dyes the dark background to after-crop the obvious green bright the bacillus tuberculosis to compare in the acid-fast stain white background the red bacillus tuberculosis to accommodate strikingly, the pyoktanin yellow O fluorescence dyes picks out limits between 0.05~0.001 Mai unit, is higher than the acid-fast stain 0.05 Mai unit, the former sensitivity is high; Moreover, concentrates the function after magnetic nano particles the specimen smear, under the mirror demonstrated that its bacillus tuberculosis quantity is higher than the specimen which obviously has not passed through concentrates.

通过本次课题，我们发现用2%NaOH-NALC消化液可在10分钟内迅速裂解痰液的粘性介质，在保证消化效果的同时不会由于NaOH过强的作用杀死细菌（时间控制在20分钟内），亦不会影响磁性颗粒的作用；通过应用自制定量模拟临床标本，我们比较了抗酸染色和金胺O荧光染色的检出限，金胺O荧光染色黑暗背景下发出明显绿色亮光的结核杆菌较抗酸染色白色背景中的红色结核杆菌更容醒目，金胺O荧光染色的检出限在0.05~0.001个麦氏单位之间，高于抗酸染色的0.05个麦氏单位，前者敏感度较高；另外，经过磁性纳米颗粒富集作用后的标本涂片，在镜下显示其结核杆菌数量明显高于未经过富集的标本。

The results show that the as-synthesized chalcone-containing copolymers are a kind of thermosensitive polymer with a lower critic solution temperature and the LCST of the copolymer decreases with the increase of the chalcone content in the copolymer. With the increase of the solvent polarity, both the maximum absorption wavelength and the fluorescence emitting wavelength of the chalcone-containing copolymers redshifts, and the intensity of fluorescence first increases, then decreases, which exhibits solvent polarity sensitive fluorescence properties.

结果表明，侧链查尔酮共聚物是一类具有低临界溶解温度的温敏性聚合物，其LCST温度随着共聚物中查尔酮含量的增加而降低；随着溶剂极性的增加，侧链查尔酮共聚物的紫外-可见最大吸收波长红移，其荧光发光波长红移并且发光强度先增强后降低，具有溶剂极性敏感的荧光特性；同时对比侧链查尔酮共聚物水溶液低温和高温下的荧光，发现低温下几乎无荧光，高温下其荧光得到明显增强，其荧光具有可逆的温度&开/关&特性。

Tenuiflora seedlings and the fine regulation mechanism of metabolism of cell membrane stability under salt stress, electrolyte leakage rate using relatively electrical conductivity method and malondialdehyde content using the thiobarbituric acid method relating to cell membrane stability were determined as the variable of physiological parameters that could reflect the status of physiological system or injury extent by stress, and the fluorescence parameters such as Fv/Fm, Fv/Fo, qP, Fv'/Fm',ΦDPⅡ, HDR and qNP were also determined by the method of fluorescence induction kinetics with FMS2- facilitative Fluorescence Monitoring System under normal condition and different degrees of NaCl stress (0.4%, 0.8%, 1.2%, 1.6%, 2%, 2.4%) after 7d.

星星草幼苗在不同浓度NaCl胁迫处理7d后，测定了叶绿索荧光参数、MDA含量和叶片电解质外渗率。

Through fluorescence spectrum and photocurrent action spectrum measurements,we found that fluorescence signal of CdS weakened after TiO_2 film was deposited CdSby electrodeposition, and accordingly improved photoelectric capability of TiO_2 filmand achieved sensitization action.

通过荧光光谱和光电流作用谱结果表明在TiO_2薄膜电极上采用电化学沉积法沉积CdS之后，CdS的荧光信号减弱，从而提高了TiO_2薄膜电极的光电性能，起到了敏化的作用。

Except for this, the others such as ionic bridge (e. g. Me〓, I〓), appositional replacement and destroying the binding site etc. is involved in also. 5. The type of BSA intrinsic fluorescence being quenched is mainly static quenching, and the nonradiative energy transfer is attributed to BSA intrinsic fluorescence being quenched also.

各种类型的外加剂对中药小分子-蛋白质作用过程的影响方式各不相同，与BSA结合引致BSA分子构象改变是外加剂参与并影响中药小分子-BSA相互作用过程的共通机制；除此共通机制外，还可能存在离子架桥、同位取代以及对BSA分子上药物结合部位的破坏等作用形式。

It has to be computed by the host for the actual clock rate.

主控制器可以用来计算实际的时钟速率。

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