查询词典 fluorescence

The results showed that: three major peaks,humuslike fluorescence peak A,proteinlike fluorescence peaks B and C appeared on the threedimensional fluorescence spectroscopy;the DOM fluorescence intensity was well related with DOC and CODMn,indicating that fluorescence dissolved organic matter content can reflect both the organic carbon content and the organic matter content by oxidation in the water in August,during the nonalgae bloom outbreak period,fluorescence intensity had a poor correlation with chlorophyll a,indicating that phytoplankton was not the main source of the fluorescent substances.

结果显示：小江回水区DOM的三维荧光光谱主要表现出3个峰，类腐殖质荧光峰A、类蛋白荧光峰B和C；DOM荧光强度与DOC、CODMn相关性较好，表明荧光溶解有机物质的含量可以较好反映水体中有机碳和可被氧化有机物的含量；在8月份非水华暴发时期荧光强度和叶绿素a相关性较差，表明DOM荧光物质主要不是由浮游植物产生。

The results show that ponceau 4R excited by light at the wavelength of 330-430 nm can generate a strong fluorescence at the 621 nm peak wavelength with its best excitation wavelength being 376 nm, amaranth excited by light at the wavelength of 300-440 nm can generate a strong fluorescence at the 643 nm peak wavelength with its best excitation wavelength being 370 nm, tartrazine excited by light at the wavelength of 280-380 nm can generate a strong fluorescence at the 565 nm peak wavelength with its best excitation wavelength being 315 nm, sunset yellow excited by light with wavelength of 310-410 nm can generate a strong fluorescence at the 592 nm peak wavelength with its best excitation wavelength being 348 nm, and brilliant blue excited by light at the wavelength of 320-390 nm can generate a strong fluorescence at the 456 nm peak wavelength with its best excitation wavelength being 350 nm.

结果表明，胭脂红在波长330～430 nm的光激发下，产生较强荧光，荧光峰值波长为621 nm，最佳激发波长为376 nm；苋菜红在波长300～440 nm的光激发下，产生较强荧光，荧光峰值波长为643 nm，最佳激发波长为370 nm；柠檬黄在波长280～380 nm的光激发下，产生很强荧光，荧光峰值波长为565 nm，最佳激发波长为315 nm；日落黄在波长310～410 nm的光激发下，产生较强荧光，荧光峰值波长为592 nm，最佳激发波长为348 nm；亮蓝在波长320～390 nm的光激发下，产生较强荧光，荧光峰值波长为456 nm，最佳激发波长为350 nm。

Secondly, we provide a two-level model to depict the one-, two-and three-photon fluorescent process and with it to calculate the one-, two-and three-photon fluorescence intensity dependence of the incident excitation intensity and time. We study the fluorescence intensity when excitation light's time distribution are square pulse、Gaussian pulse and sech pulse. We find that the influence of excitation light's time distribution on the two- and three- photon fluorescence peak intensity is larger than the one-photon fluorescence peak intensity. Two-level model can account for the behavior of saturation fluorescence.

然后用二能级模型解释了单、双、三光子激发原理，在理论上推导出了单、双、三光子吸收诱导荧光强度与脉冲强度以及脉冲作用时间的关系，并进一步研究了入射光时间分布分别为矩形、高斯型、双曲正割型时激发溶解在氯仿溶液中的C22H22N4O样品所产生的荧光峰值光强，结果表明激发光的时间分布对双、三光子荧光峰值强度的影响比对单光子的影响要大。

To establish new methods for highly sensitive time-resolved fluorescence bioassay, several new lanthanide fluorescence complexes were synthesized and used for the preparations of nanometer-sized lanthanide fluorescence biolabels. Novel lanthanide fluorescence nano-biolabels are uniform (25-55 nm in diameters),strongly fluorescent with long fluorescence lifetime, highly photostable and biocompatible.

本项目以时间分辨荧光生物分析新方法的建立与应用为研究目的，在合成出几种稀土荧光配合物的基础上，建立了几种新型强荧光性纳米稀土荧光生物标记材料的可控制备方法，生物标记方法及其在时间分辨荧光免疫分析中的应用方法。

To establish new methods for highly sensitive time-resolved fluorescence bioassay, several new lanthanide fluorescence complexes were synthesized and used for the preparations of nanometer-sized lanthanide fluorescence biolabels. Novel lanthanide fluorescence nano-biolabels are uniform (25-55 nm in diameters),strongly fluorescent with long fluorescence lifetime, highly photostable and biocompatible.

中文摘要：本项目以时间分辨荧光生物分析新方法的建立与应用为研究目的，在合成出几种稀土荧光配合物的基础上，建立了几种新型强荧光性纳米稀土荧光生物标记材料的可控制备方法，生物标记方法及其在时间分辨荧光免疫分析中的应用方法。

And a fluorescence molecule beacon probe is designed between upstream and downstream priers, 5' end of probe can mark fluorescence reporter group FAM, and the 3' end markes fluorescence quenching group DABCYL, and 5' end and 3' end of the probe have 6 bases which can be reflected and complemented to make fluorescence molecule beacon probe do not give out fluorescence signal in free state.

在上下游引物中间设计一条荧光分子信标探针，探针的5'端标记荧光报告基团FAM，3'端标记荧光淬灭基团DABCYL，探针的5'端和3'端有6个碱基能反折互补，使荧光分子信标探针在游离状态下不发出荧光信号。

The results indicate that the weak election-withdrawing group 4-hydroxymethyl in 4-position of pyridine in 4-HMDPA could weaken the fluorescence intensity of the lanthanide complexes. The contradistinctive experimental results show that the fluorescence intensities of these complexes are related to pH value of the aqueous solution and the dipole moment of solvent molecule: in the neutral aqueous solution,the fluorescence intensities of these complexes were strongest,while the less the dipole moment was,the stronger the fluorescence intensity was.

结果表明，在吡啶4位引入弱吸电子基团4-羟甲基会减弱稀土配合物的荧光强度；在水溶液中稀土配合物与溶液的pH值有着密切的关联，中性水溶液中荧光强度较大；分子偶极矩较小的溶剂中稀土配合物荧光强度较强。

Pyronine B adheres to on the surface of CdS and prevents CdS aggregating, which leads to the decrease of fluorescence quantum yield of CdS. Fluorescence resonance energy transfer happens from CdS to Rhodamine B, Butyl rhodamine B and Rhodamine 6G, respectively. There is a higher FRET efficiency between CdS and Neutral red with the value being 0.282. Moreover, coupling reaction between -NH_2 of Neutral red and -COOH of CdS -TGA leads to red shift of CdS fluorescence spectrum. The reaction also occurs between Safranine T and CdS. At the same time, the Fluorescence of ST is quenched by forming ST -CdS ground state complex. Chapter 6, CdS QDs modified by chitosan and polyethyleneimine can be quenched by Cu~(2+). According to the experimental result, we develop the novel sensor for Cu~(2+).

研究结果，吡啰红与CdS之间作用形式是染料聚集在CdS的表面使得CdS不易聚集，但同时也降低了CdS的发光的量子效率；罗丹明B、罗丹明6G、丁基罗丹明B均与CdS之间存在能量转移，位阻效应使得能量转移效率最高的BRhB的相对荧光强度增大最小；中性红与CdS的能量转移效率更高，同时推测可能使CdS-TGA上的-COOH与部分NR的-NH_2发生偶联导致粒子变大而发生荧光光谱的红移；番红花红与CdS是相互荧光猝灭，可能是ST的-NH_2与CdS-TGA上的-COOH发生偶联使得纳米粒子变大以及二者形成基态复合物的共同作用的影响。

To investigate the fluorescence dynamic change of protoporphyrin IX in bladder carcinoma cell induced by 5-ALA and the influence of 5-ALA concentration and pH on fluorescence of PpIX. 2. To discuss the biodistribution and express of PpIX in bladder wall of different lesions by method of instilling of 5-ALA into bladder cavity. 3. To discuss the clinical value of the fluorescence diagnosis in early detection of bladder cancer and the radicality of bladder tumor resection under fluorescence cystoscopy.

本研究目的：1了解5-ALA诱导膀胱癌细胞内生原卟啉IX荧光随时间的动力学变化及5-ALA的浓度、pH值对产生PpIX荧光的影响。2通过膀胱灌注5-ALA探讨其所诱导内生PpIX荧光在膀胱上皮中分布及不同病变表达上的差异。3探讨5-ALA诱导荧光膀胱镜检对发现早期膀胱癌的临床应用价值及在荧光膀胱镜下膀胱肿瘤电切治疗的彻底性。

In this thesis, the resonance fluorescence spectra of a A-type three-level atom is calculated, which is driven by two bichromatic fields having the same difference of the frequency. In addition, the influence of the relative phase difference on the fluorescence spectra are considered. It is found that the fluorescence spectra of the atom are deeply depend on the relative difference and the appearance and cancellation of the fluorescence are obtained by changing the relative phase difference.

文中以具有相同频差的两个双色场驱动下的三能级Λ型原子为模型，计算了其共振荧光谱，考虑了两个双色场的相对相位差对谱线的影响，结果表明：原子的荧光谱强烈地依赖于驱动场的相对相位差，可以利用相对相位差的改变对荧光的产生和抑制加以操纵。

Panorama Many people are dancing in a large sitting room.

在一个大客厅里，许多人在跳舞。

We fully understand how a microwave oven cooks food, it is first necessary to talk ...

我们充分理解一个微波炉煮的食物，这是首先要谈。。。