查询词典 fluorescence

The results of its intrinsic fluorescence spectroscopy and fluorescence phase diagram showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L, there existed a partially folded intermediate of Bacillus amyloliquefaciens a-amylase during its unfolding procedure, which followed a three-state model; the result of its fluorescence probe showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L, there existed some stable hydrophobic regions, which could interact with a hydrophobic reagent 8-anilino-1-naphthalene sulfonic acid, in the partially folded intermediate of Bacillus amyloliquefaciens a-amylase; and the results of fluorescence quenching using acrylamide and potassium iodide as quenchers showed the distribution of Trp residues in Bacillus amyloliquefaciens a-amylase in different denaturation solution, with the maximum number (8) of tryptophan residues in a partially folded intermediate Bacillus amyloliquefaciens a-amylase molecule could be quenched by potassium iodide; and the results of their protein electrophoresis and SEC showed that no aggregate or aggregate precipitation of Bacillus amyloliquefaciens a-amylase formed during the whole unfolding procedure of Bacillus amyloliquefaciens a-amylase induced by guanidine hydrochloride.

内源荧光光谱和荧光相图结果表明，当变性液中盐酸胍浓度约为1.0 mol/L时，芽孢杆菌a-淀粉酶的去折叠过程中出现一个部分折叠中间体，其去折叠过程符合&三态模型&；荧光探针结果表明，在溶液中盐酸胍浓度约为1.0 mol/L时，中间态芽孢杆菌a-淀粉酶分子中存在着能够与探针分子1-苯胺基-8-萘磺酸结合的稳定的疏水区域；荧光猝灭研究给出了不同程度变性的淀粉液化芽孢杆菌a-淀粉酶中的Trp的分布情况，结果表明中间态芽孢杆菌a-淀粉酶分子中能够被碘化钾猝灭的位于分子表面的色氨酸残基数目达到最大的8个；蛋白电泳和体积排阻色谱结果表明，在盐酸胍诱导的芽孢杆菌a-淀粉酶分子的整个去折叠过程中，不会以共价键或非共价键形式形成芽孢杆菌a-淀粉酶分子之间的集聚体或集聚体沉淀。

At 0-60 ℃, the maximum fluorescence intensity of GCS-EPF decreased on heating and the relationship between fluorescence intensity and the change of temperature was linear; At pH=0-13.5, its fluorescence intensity increased with basification and completely disappeared at very acidic pH.

结果表明，修饰后水溶性壳聚糖的水溶液、固体粉末和薄膜在520 nm附近具有较强的荧光发射，其荧光强度不仅在0-60 ℃时对度有较快敏感响应，同时在pH=0-13.5时对pH也有较快敏感响应，具有双重敏感响应，因此可将其作为温度荧光探针和pH荧光探针的高分子材料。

Sunset clouds has comfortable pragmatic simple furniture within the red floor house , living room white hangs on the wall to oil painting having personage or scenery, bedding has the bilayer milk white quilt on bedroom 180 CM * 200 CM bed , both sides readjusts oneself to a certain extent the desk lamp having two Bixa orellana pole light yellow modelled after antiques charpie lamp shade to may adjust luminosity on the head of a bed on the bedside cupboard , the bedroom *allpox gushes the coating material blue seabed pattern having fluorescence, the queen who puts out the light in the evening may issue fluorescence , fluorescence is a dolphin and a group of fingerling.

晚霞红地面的屋内有简单舒适实用的家具，客厅白色墙上挂有人物或风景的油画，卧室180CM*200CM床上铺垫有双层奶白色被子，床头两旁床头柜上放有两盏红木仿古支杆淡黄绒布灯罩可调节光度的台灯，卧室的天花喷有荧光的蓝色海底图案的涂料，晚上熄灯后可发出荧光，荧光是一个海豚和一群小鱼。

CWhen the fusion protein was co-culture with nematode,the analysis of fluorescence microscope showed the digestive system and the coelom of nematode could be detected green fluorescence, and the fluorescence intensity was time-and protein concentration-dependent.

其三，融合蛋白喂食线虫发现蛋白进入其整个消化道及其原体腔，且与喂食时间与蛋白浓度呈正相关关系。

Fig 1 The results of two color FISH A:Normal sperms(a sperm nucleus with a red fluorescence domain was X sperm, and with a green fluorescence domian was Y sperm);B:Numeric chromosome abnormal sperm two green fluorescence domains within the same sperm nucleus which may be classified in Y disomic sperm or diploidy sperm, in the case of hybridization with two probes, one euchromosome specific and the other sex chromosome specific, this sperm could be discriminated as disomic or diploidy sperm

图1 双色荧光原位杂交结果 A：正常精子(精子头部有1个红色荧光信号者为X精子，有1个绿色荧光信号者为Y精子)；B：染色体数目异常精子两个绿色荧光信号同在1个精子核内，可为Y双体精子或二倍体精子，若用1条常染色体探针和1条性染色体探针进行双色荧光原位杂交，可以区别该精子属Y双体精子或二倍体精子

In the field condition, PAM-2100 chlorophyll fluorometer was used to observe chlorophyll fluorescence kinetics parameters of 18 winter wheat varieties in different growth stages, the correlations of 14 fluorescence parameters such as Fo were analyzed. Fluorescence characteristics of winter wheat varieties were comprehensive evaluated by the methods of factor analysis and cluster analysis.

在大田条件下应用PAM-2100型叶绿素荧光仪对18个冬小麦品种叶绿素荧光动力学参数进行了不同生育期的观测，分析冬小麦叶绿素荧光参数Fo等14个荧光参数间的相关关系，利用因子分析和聚类分析对其冬小麦品种荧光特性进行综合评价。

At different temperatures,the binding of naphthol green B to bovine serum albumin was studied by the fluorescence spectroscopy,three-dimensional fluorescence spectrum,synchronous fluorescence spectrum and ultra-violet spectrum.

在不同温度下，研究了萘酚绿B作用于牛血清白蛋白的荧光猝灭光谱、同步荧光光谱、三维荧光光谱和紫外-可见吸收光谱特征。

The result shows that ponceau 4R has four obvious fluorescence spectral peaks respectively located at 420,530,635 and 687 nm,each of these fluorescence spectral peaks has different best induced light,and the corresponding fluorescence spectra were listed.

文章分别从理论与实验上对220-400 nm不同激发波长下胭脂红标准溶液的荧光光谱进行了分析，结果表明，胭脂红可以产生较强的荧光，分别在420，530，635，687 nm波长处产生了4个荧光峰，各荧光峰的最佳激发波长也不尽相同，文章给出了相应的荧光光谱图。

The fluorescence properties of DAKGM solutions were studied in detail.The fluorescence properties of DAKGM solutions were studied via steady fluorescence spectra. Two emission peaks appeared at 425 and 465 nm, respectively.

过渡金属离子Fe3+和Cu2+能显著降低KGM的荧光强度，根据Stern-Volmer方程得到了Fe3+和Cu2+对KGM的淬灭常数分别为203.8 L/mol和171.0 L/mol.3、系统地研究了醛基葡甘聚糖溶液的荧光光谱特性。

Methods A lamellar flap was produced in the rabbit cornea with a microkeratome. The flap interface in whole corneas and corneal beds were soaked in the rAAV –EGFP solution for 10 min, BSS solution was applied in the control group. The corneas were harvested at day 1, 3, 14, and 21 after application for the structure analysis of cornea by HE staining. Expression of the transferred gene was monitored by fluorescence stereoscope and laser confocal microscope. Results After 3d of infection with rAAV-EGFP ,slight fluorescence was detected in rabbit cornea by fluorescence stereoscope .

微型角膜刀制作兔角膜基质瓣；转染组用含rAAV－EGFP转染液浸泡角膜瓣和角膜基质床10 min，对照组用等量BSS液浸泡角膜瓣和角膜基质床10 min，不同时间点(1、3、7、14、21d)通过荧光体视镜观察角膜出现EGFP荧光的起始时间及分布情况，收集角膜，其中一半用激光共聚焦显微镜观察、照相，另一半固定后行HE染色。

Panorama Many people are dancing in a large sitting room.

在一个大客厅里，许多人在跳舞。

We fully understand how a microwave oven cooks food, it is first necessary to talk ...

我们充分理解一个微波炉煮的食物，这是首先要谈。。。