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B order to make full use of Ta1, it is essential to improve the investigation to industrial production scale by drawing assistance from high cell density fermentation. Pilot test of tube culture and flask shaking culture were done to get optimized culture conditions. Finally, we reached the goal of high cell density fermentation and high level expression of recombinant human Ta1 E.

在经过试管以及三角摇瓶水平对培养条件进行摸索后，我们成功地实现了该工程菌的高密度发酵，3乙菌液获得湿菌体工4口丁蛋白表达水平与试管水平相当。

Compared with shaking flask cultivation, Tobramycin fermentation in 5L auto fermenter has been studied systematically.

分析和对比了不同溶氧情况下妥布拉霉素菌株在摇瓶和5L全自动发酵罐中发酵过程的特征。

Fermentation processes in shake flask and 15 L fermentor were obtained.

得到了摇瓶发酵和15 L发酵罐中的发酵工艺。

The effects of scale-up from shake flask to fermentor on taxane formation by T.

研究和探讨了从摇瓶到生物反应器的放大对中国红豆杉悬浮细胞生产紫杉烷的影响。

Than that of fermentation in shake flask fed batch and 5-liter fermentor batch.

在此基础上进行了5L发酵罐补料分批发酵试验。

In order to provide some useful information for large-scale production of taxol by plant cell cultures, the emphases of this study were put on the high density cell cultivation and the effects of scale-up from shake flask to fermentor on taxane formation.

论文的重点是实现中国红豆杉悬浮细胞的高密度培养，并考察和探讨了该细胞株在生物反应器培养过程放大中存在的一些相关问题。

I would like you to go and get me a flask of hot water.

我想请你给我拿一瓶开水来。

I would like you to go get me a flask of hot water.

我想请你给我拿一瓶开水来。

I'm sorry that your flask is empty.

我想请你给我拿一瓶开水来。

I want to drink a flask of wine.

我想喝一瓶酒。

I use an example quoted by Hu Jiaqi:"It is reported that America separates the DNA of a kind of virus by making use of genetic technology and combines it with another kind of DNA. Finally, they get a kind of virulent biological agent called a "pyrotoxin". Someone discloses in private that 20 grams of such a kind of biological agent could result in the global death of 6 billion people due to infection."

我在这里例举胡家奇所引用过的例子："据报道，美国利用转基因技术，将一种病毒的DNA分离出来，与另外一种DNA进行结合，拼结成一种剧毒的"热毒素"生物战剂，且私下有人透露，这种生物战剂只需20克，就可以导致全球60亿人全部感染死亡。"

Waiting, for the queers and the coons and the Reds and the Jews.

等待着疯子和黑人还有红色共产主义者还有犹太人