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The recombinants with different multiple copies of amyL integrated in the chromosome were identified by real-time PCR and evaluated by shake-flask fermentation.

对转化子的amyL拷贝数及其BLA发酵水平分别用荧光实时定量PCR及摇瓶发酵试验进行评价与鉴定。

Methods: Mesenchymal stem cells were isolated from human term placenta by digestion of collagenase Ⅱ and their unique growth characteristic of attaching to the wall of cell culture flask. The proliferation ability was detected by living cell number counting and propidium iodide staining. Their surface markers were detected by flow cytometry. The cells were induced to osteoblast with dexamethasone, antiscorbutic acid and β-sodium glycerophosphate. And they also were induced to adipocytes with dexamethasone and insulin. After induction, the cells were observed by Von Kossa staining and oil red O staining.

将人足月胎盘组织经胶原酶Ⅱ消化和贴壁培养法获取间充质干细胞，运用活细胞计数和碘化丙吮检测其增殖能力；采用流式细胞术检测其细胞表面标志的表达；用地塞米松、抗坏血酸及β-磷酸甘油诱导其向成骨细胞分化，并用Von Kossa染色进行鉴定；用地塞米松与胰岛素诱导其向脂肪细胞分化，并以油红O染色进行鉴定。

Filter the separated aqueous layer, wash the diphenyl ether with 5.0ml of ice-cold water, pass the washings through the filter, and collect the filtrate and washings in a 25-ml volumetric flask.

过滤已分离的水层，用5.ml的冰水洗涤二苯醚，合并滤液和洗涤液。

In this paper, contrast flask bacterial tests of high arsenic gold concentrate with arsenopyrite type and pure arsenopyrite mineral showed that the pyrite in the concentrate promoted the efficiency of dearsenication rate of the mineral, it was significantly higher than pure arsenopyrite.

通过毒砂型高砷金精矿和单矿物纯毒砂的氧化对比摇瓶试验，发现由于金精矿中黄铁矿的存在促进了矿物的脱砷效率，脱砷速度明显高于纯毒砂。

If a water run vacuum aspirator is used, irregularities in the water flow may cause a backflow of air or water into the vacuum flask, introducing contaminants to the filtrate.

如果水运行外太空使用中的不正常现象水流可能会导致回流的空气或水进入保温瓶介绍污染物的滤液。

Secondly, TheOHCs body possess the flask-shaped in the basal turn of the cochlea, which don"t likeother mammals"tube-shaped OHCs body. All of the results above indicate that bats andmostly any other little mammals have the similar audiogram in definite range, but alsohave some difference. Echolocating bats always possess of acuity hearing, whichinclude more available frequency.

以上研究结果表明蝙蝠和大多数其他小型哺乳动物在一定范围内虽有着相似的听力敏度图，却还是有不同之处的，回声定位蝙蝠经常拥有对它们来说更为有用频率的敏锐听力。

He believed that one should never learn from a book what one could learn from a beaker, flask, or Bunsen burner.

他相信，学生从书本上是无法学习到那些在操作烧杯、烧瓶和煤气灯中学到的内容。

Everybody had its own "output" of alcohol – a flask, a carboy or some can… Aviators visited us: Guys, we brought presents for you – the leather pilot's jackets.

每个人都有自己的"产量"—用小玻璃瓶、大瓶子或者是罐头…。。飞行员会来找我们兄弟们，我们给你们带来了礼物—飞行员皮夹克。

The soil bacteria was well dispersed after the Dowex resin, sodium cholate, glassware and soils were placed into a flask and the suspension was shaken for 2h .

采用0.1%胆酸钠、钠型离子交换树脂、玻璃珠与土壤样品一起在4℃振荡2h，能够较好的分散土壤中的细菌。

The influence of dissolve oxygen to the yield of coenzyme Q_(10) in flask culture was studied.

考察了发酵过程中溶氧对辅酶Q_(10)产量的影响。

Foods high in this vitamin include oily fish such as salmon, tuna and, and fish liver oils.

维生素D含量高的食物包括诸如大麻哈鱼、金枪鱼和鲭鱼之类的油鱼以及鱼肝油。

We need you to cosign our public letter demanding that press intimidation cease immediately, and that all charges be dropped.

我们需要您cosign我们的公共信，要求记者立即停止恐吓，并要求所有收费下降。