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Four strains BL-11, BL-12, BL-21, BL-22 were identified to be Bacillus laterosporus. They were inoculated into a 250 mL Erlenmeyer flask containing 50 mL of the media with organophorus pesticide as the only phosphorus source.

菌株BL-11、BL-12、BL-21、BL-22是自行分离得到的四株具有解磷能力的细菌，经鉴定为侧孢芽孢杆菌。

After cultivated in the optimized medium (soy power 1.5, corn power 1.5, NaCl 1,K2HPO4 0.01, KH2PO4 0.01, CaCl2 0.4, span40 0.1,pH 10.0) at this condition (50℃,with shaking 180r/min) for 72 h in 100 mL erlenmeyer flask, the activity ofα-amylase obtained 105U.

经优化培养基(g/100 mL)(黄豆粉1.5，玉米粉1.5， NaCl 1， K2HPO4 0.01， KH2PO4 0.01， CaCl2 0.4，司盘40 0.1，pH 10.0)在50℃、装液量10%、180 r/min的摇床培养72 h后，测得酶活105 U。

The optimum fermentation conditions for HA07009 were initial pH7.0, seed age 48h, 8% inoculation amount, and the size 75 ml in 250ml Erlenmeyer flask at 200r/min, 6d.

发酵培养条件为：初始PH7.0，种龄48h，接种量8%，摇床转速为200r/min，250ml三角瓶装液量75ml，发酵培养时间6d。

In volumetric analysis, we use many glass vessels such as mortar, test tube, beaker, separate funnel, grade cylinder, pipette, Erlenmeyer, buretteand volumetric flask to ground, dissolve, separate, pipe, titrate samples or dilute samples to volume.

在容量分析中，我们使用很多玻璃容器，如迫击炮，试管，烧杯，单独的漏斗，职缸，移液器， erlenmeyer滴管和容积烧瓶至地面，溶解后，就分开，管，滴定样品或稀释样本量。

The optimal fermentation conditions for Penicillium sp. D-l producing chitosanase were incubated at 32, in 500ml Erlenmeyer flask containing 100ml medium (pH 5.0) with 2.5% chitosan as substrate.

通过研究，确定了Penicillium sp.D-1产酶的最适发酵条件：培养温度32℃，底物壳聚糖浓度2.5％，装量为500ml三角瓶装100ml培养基，培养基pH5.0。

The optimum inoculation conditions for HA07037 were initial pH7.0, seed cultural time 48h, 10% inoculation amount, and the size 75ml in 250ml Erlenmeyer flask at 200r/min, 6d.

发酵培养条件为：初始pH7.0，种龄48h，接种量10%，摇床转速为200r/min，250ml三角瓶装液量75ml，发酵培养时间6d。

In volumetric analysis, we use many glass vessels such as mortar, test tube, beaker, separate funnel, grade cylinder, pipette, Erlenmeyer, buretteand volumetric flask to ground, dissolve, separate, pipe, titrate samples or dilute samples to volume.

在容量分析中，我们使用很多玻璃容器，如迫击炮，试管，烧杯，单独的漏斗，职缸，移液器， erlenmeyer滴管和容积烧瓶至地面，溶解，就分开，管，滴定样品或稀释样本量。

In this experiment, 250mL Erlenmeyer flask with 50mL reaction liquid(400mmol/L glucose, 10mmol/L CMP, 50mmol/L choline phosphate, 100mmol/L K 2HPO 4 KH 2PO 4, 10mmol/L MgSO 4, pH=8.0) and 1 200g/L immobilized cells were used. After 4h incubation 400mmol/L of glucose was supplemented.

结果表明：2 5 0 m L摇瓶中加入 5 0 m L反应液(葡萄糖 40 0 mmol/ L，CMP1 0 mmol/ L，磷酸胆碱 5 0 mmol/ L，K2 HPO4 -KH2 PO4 缓冲液 1 0 0 mmol/ L，Mg SO4 1 0 mmol/ L，p H=8.0 )，固定化细胞 1 2 0 0 g/ L，3 4°C、1 5 0 r/ min下，反应 4h后补加 40 0 mmol/ L葡萄糖，反应 8h可使60 %以上的 CMP转化为 CDP-胆碱。

The optimum culture medium components of DA07405 contained glucose 1.0%, sucrose 0.5%, corn powder 0.5%, soybean powder 0.75%, K2HPO4 0.05%; The optimum fermentation conditions of DA07405 were initial pH7.0, seed age 48h, 12% inoculation amount, the size 75mL in 250mL Erlenmeyer flask at 200 r/min and 28℃ for 5d.

确定菌株DA07408的最佳培养基配方及发酵条件为：葡萄糖1.0%，甘油1.5%，玉米粉0.75%，大豆粉0.75%，K2HPO4 0.05%，初始pH值7.0，种龄48h，接种量10%，200r/min发酵5d，培养温度28℃，250mL三角瓶装液量75mL。

With the single factor test and orthogonal test, the optimal culture media for HF3-04 strain producing agarase was decided as: agar 3‰, NH4NO31‰, Na2HPO4 0.5 mM, pH 8.0. The optimal fermentation conditions were inoculum size 3‰, 110ml medium in 250mL Erlenmeyer flask, rotation rate 150r∕min, culture time 48h,culture temperature 25℃.

通过正交试验确定其最佳培养基组成为琼胶3‰、硝酸铵1‰、磷酸氢二钠0.5mM、pH值8.0；该菌株的最佳培养条件为接种量1%、装液量为250ml三角瓶装入110ml发酵培养基、摇床转速150r∕min、培养温度25℃、培养时间48h。

If you were not , and OS X just booted normally, have no fear.

如果你没有，和OS X刚刚启动通常，没有恐惧。

I only want to preserve our all .

我只想保存好我们的一切