查询词典 fermentation method

One integrative transformant was obtained through PCR and phenotype identification, designated as A.oryzae ONL1. The 7-day fermentation broth of A.oryzae ONL1 could hydrolyze tributyrin verified by forming transparent cleared zone on tributyrin plate, and the enzyme activity reached 2.5 U/mL. The SDS-PAGE electrophoretic map of the fermentation broth demonstrated that there was a typical RML band at 32.5 kDa.

其培养7天的培养液上清在以三丁酸甘油酯为底物的平板上形成清晰的水解透明圈，碱滴定法酶活测定表明培养液酶活可达2.5 U/mL，培养液上清的SDS-PAGE图谱在32.5 kDa处有RML的特征条带。

During the stationary phase of fermentation, cell productivity was slightly reduced. MgSO_4 and tryptone have the positive effect on the product synthesis and cell growth respectively. Based on these conclusions, MgSO_4 and tryptone were added to the feed medium to enhance the production of polysialic acid. Variable feed rate policies were applied to the fed-batch fermentation process and the final biomass and polysialic acid production were both increased by 40 percent.

通过摇瓶实验研究了大肠杆菌发酵过程中菌体生长和产物合成的影响因素，结果表明：产物合成与菌体生长是相耦联的过程，菌体生长受到菌体代谢产物的抑制作用，聚唾液酸的合成不受代谢产物的抑制作用；发酵进入稳定期后菌体的生长和生产能力有所降低；硫酸镁和胰蛋白胨分别对产物合成和菌体生长有一定的促进作用。

The medium composition and some fermentation parameters were studied by cross test and single factor experiments. The optimal conditions are as flows: Tryptone 0.76 %, Bean powder 1.0 %,initial pH 8.0, temperature 28 ℃, inoculation quantity 8 % and loading volume 30 %. Under optimal conditions, the maximum yield of biomass and microbial lipids reached 29.8 g/L and 11.7 g/L, respectively, which was 2.59 times and 2.05 times of those cultured in the original medium, after 9 days fed-batch co-fermentation in shake-flask.

采用单因子和正交实验对培养基组成和发酵条件进行了优化，结果表明，诱变菌在温度28 ℃，pH 8.0，接种量8 %，装液量30 %，蛋白胨0.76 %和豆粕1 %，分批补糖的最优发酵条件下发酵9 d，生物量和菌体油脂积累量分别达29.8 g/L和11.7 g/L，较出发菌提高了2.59倍和2.05倍，同时原料糖利用率达到99.4 %。

Due to the gradually in-depth study on gene, biological fermentation technology and strain, as well as the resistance to the chemical synthetic product, the bio-engineering fermentation technology achieves unparallel development and is widely applied in the fields of medicine, food and chemical engineering.

由于人类对基因、生物发酵技术、菌种研究的逐步深入，以及人们对化工合成产品的抵制，生物工程发酵技术得到了空前的发展，并在医药、食品、化工领域得到了广泛的应用。

Tile cytotoxicity of fermentation broth from 134 strains of marine actinomycetes on P_(388) and KB cancer cells was evaluated and the result showed that about 16% of fermentation broths had ID_(50) at or above 320,of which the highest ID_(50) is 20480,indicating that marine actinomycetes,which produced antitumor agents with strong cytotoxicity,are the potential source of ...

对434株海洋放线菌发酵液的筛选结果发现，约16％的海洋放线菌发酵液对P_（388）和KB癌细胞的ID_（50）等于或大于320，其中最高可达20480。这表明海洋放线菌存在着活性高的细胞在抗肿瘤活性物质，是潜在的抗癌药源。

The jujube culls is developed using enzyme hydrolysis,yeast fermentation and acetic fermentation to produce jujube vinegar.

利用枣制品下脚料枣液，使其通过酶解、酵母发酵、醋酸发酵等工艺研制枣醋。

The processing technology of Huanghua pear fermented wine are : Huanghua pear →selection, washing →cleavage breakage→pretreatment→squeezing juice →pectinase clarification(pH3.5,1%pectinase solution adding 0.15%,45℃,50minutes)→dominant fermentation (1596 yeast, temperature 20℃,inoculation volume 7%,optimum initial pH3.1~3.4,volume loading 80%,sulfur dioxide 100mg/l,circle 8 days )→seperation and taking wine →after fermentation(10~15℃,14days)→seperation and disgorging→maturation→clarification (bentonite adding 0.16%)→filtration→adjustment→bottle

9黄花梨酒的加工工艺为：黄花梨→挑选、清洗→破碎→预处理→榨汁→果胶酶澄清(pH3.5，1％酶液的加入量为0.15％，温度45℃，时间50min)→主发酵(1596菌种，发酵温度20℃，接种量7％，最适初始pH3.1～3.4，装液量80％，二氧化硫加入量100mg／l，周期8天)→分离取酒→后发酵(10～15℃，时间14天)→分离除渣→陈酿→澄清(皂土加入量为0.16％)→过滤→调配→装瓶

Prediction approach of potentiality for environmental factors pH,temperature and dissolution oxygen on yeast fermentation was proponed,Based on constructing the membership functions of environmental factors on fermentation process,the fuzzy relation equation model was formed from fuzzy evaluation vector,membership grade matrix and factor weight vector.

提出一种预测环境因子pH、温度和溶氧对酵母发酵影响潜力的方法。在构造环境因子对发酵过程评价的隶属函数的基础上，建立由模糊评价向量、隶属度矩阵和因子权重向量构成的模糊关系方程模型。

However, the conversion rate of glycerol produced from the first stage using cornstarch to 1,3-propanediol from the second stage was only 26.5 % in 31h and the final concentration of 1,3-propanediol was 13.4 g/L. The productivity of 0.43 g/ of the second stage was much lower than that of a two-stage-fermentation from glucose （1.65g/ L） or of single fermentation from saponified glycerol, which is

多年来，研究人员在甘油生物歧化过程的代谢途径、动力学特征等方面已进行了较为深入的研究，甘油作为唯一碳源的最大理论摩尔转化率为72％，辅助底物的使用可以将甘油转化率提高至100％，发酵液中1，3一PD的最终浓度可以达到65～70 g／L3 。。。。。。

If a malo-lactic fermentation is desired, the wine remains on the lees without sulfating until the mali-lactic fermentation has taken place.

如果需要进行苹果酸-乳酸发酵，沉淀物上的酒体不需要硫化处理，直到完成苹果酸-乳酸发酵。

Pachomius manual labour was organized as an essential part of the monastic life; and since it is a principle of the monks as distinguished from the mendicants, that the body shall be self-supporting, external work of one sort or another has been an inevitable part of the life ever since.

根据圣pachomius体力劳动组织作为一个重要组成部分寺院生活；以来，这是一个原则的和尚，作为有别于乞丐，该机构应自支撑，对外工作的这种或那种已一个必然的一部分，生活至今。

In order to study the effects of parametrical rolling on the ship movement and tilts, in allusion to ship's navigating in regular following waves, the equation of ship rolling motion is transformed into normal Mathieu equation by proper variable replacement when strong parametrical excitation in restoring moment varied according to sine or cosine wave.

为研究参数横摇对船舶运动和倾覆的影响，通过对船在规则波上随浪航行时，恢复力矩中出现的1个以正余弦规律变化的强参数激励项进行适当的变量置换，将船舶的横摇运动方程转换成标准形式的Mathieu方程。