查询词典 expression mark

It will provide the rational for exploring the ideal treatment for liver cancer by detecting the expression of c-Met,hTERT and MAGE-1,which has high expression in liver cancer,in cancer tissues and tissues surrounding tumor by immunohistochemistry,analyzing the different expression of gene in tumor tissue and tissues with different distance from tumor,and then initially debate the molecular boundary in liver tumor exairesis.

通过免疫组织化学方法检测原发性肝细胞癌组织中高表达基因c-Met、hTERT、MAGE-1的表达以及它们在肝癌癌周组织中的表达，分析基因在肝癌组织和癌周不同距离组织的差异表达，初步探讨肝癌的分子切缘，为探索新的治疗肝癌途径奠定坚实的理论基础。

It is a little of expression in the physiological condition,while stronger expression under pathological.The high expression of glial fibrillary acidic proteinis a marker of activation of astrocyte.

GFAP的表达增强是Ast活化增殖的特征性标志，在神经系统发育及病理过程中发挥重要作用，对神经元的存活、突起的再生和神经系统功能的恢复有重要的影响。

The high expression of mmp-2 and cd44v6 were positively correlated with lymph node metastasis and fibromembranous infiltration （p＝0.011, p＝0.034和p＝0.022, p＝0.001）.the expression of mmp-2 was positive relation to that of cd44v6 （r＝0.427,p＝0.002）.conclusion the detection of expression about mmp-2 and cd44v6 in esophageal carcinoma may be helpful to judge the malignant behavior,metastasis and progronsis.

食管癌组织中mmp-2表达与cd44v6表达呈正相关（r＝0.427，p＝0.002）。结论 mmp-2和cd44v6表达是食管癌发生与发展的重要促进因子，其过度表达与食管癌浸润转移密切相关。检测mmp-2和cd44v6蛋白表达可作为判断食管癌预后的客观指标。

The expression of ILK in the preretinal membranes in PDR indicate that ILK involves in the pathological process of neovascularization in PDR. Intravitreal injection of Avastin can decrease the numbers of endothelial cells in the proliferative fibrovascular membranes, and it also can down-regulate the expression of VEGF and HIF-1αprotein, but it can not affect the expression of ILK protein.

结论1。人PDR增殖膜中的新生血管有ILK表达，提示ILK参与了视网膜新生血管的形成过程；玻璃体内注射Avastin治疗PDR，能够减少视网膜新生血管内皮细胞数量，下调HIF-1α、VEGF在增殖膜的表达，但不能下调ILK的表达。

The results showed that when both β-actin and GAPDH were used as internal control the MYH mRNA expression was complete consistent, and its expression was increased from the gastrula stage to larval stage, which represented the statistic significant difference at the level of 0.05 possibility. However, the level of mRNA expression during the developmental stages was instable when 18S rRNA was used as internal control gene.

研究结果表明，以β-actin和GAPDH作为内参，MYH基因mRNA表达水平完全一致，其表达量从原肠到仔鱼阶段逐次递增，仔鱼与原肠期阶段相比表达量差异显著；当采用18S rRNA作为内参时，MYH基因mRNA在发育阶段的表达量呈不稳定状态。

For resolving the two questions, based on criticism to anterior theory of expression in his early stage, Merleau-Ponty creates the theory of gestural expression which considers that act of body has its meaning, and it is original act of expression, and speech as a kind of act of body has its meaning.

为解决这两个问题，在早期，他在批判旧的表达理论的基础上，创建了身体姿势表达理论，认为身体动作自身就蕴含了意义，是一种原初的意义表达行为，而言语就是一种身体动作，蕴含了自身的意义。

We aim to screen and identify the key potential trans-factors during hemoglobin switch. We firstly analyzed differential expression of mRNAs in erythroid induction cultures of CD34+ cells derived from normal umbilical cord blood, adult bone marrow, and bone marrow of a heterocellular hereditary persistence of fetal hemoglobin patient. We identified ZF (HCF-binding transcription factor, Zhangfei) and SH3GLB1(SH3-domain GRB2-like endophilin B1) that had differential expression in the above three cultures. Furthermore, we confirmed the different expression of the above two genes by quantitative real-time PCR.

为鉴别影响珠蛋白基因表达和开关的新的重要调节因子，我们首先分析了人脐带血、正常成人骨髓和异细胞型胎儿血红蛋白持续存在综合症患者骨髓细胞红系诱导培养物中基因表达的差异，发现ZF(HCF-binding transcription factor，Zhangfei)和SH3GLB1（SH3-domain GRB2-like endophilin B1）在这三种不同来源的红细胞内具有显著的表达差异，并通过实时定量PCR方法验证了差异的真实性。

After the NK gene from recombinant plasmid pUC19-NK was cloned on expression vector pBV220, it was transformed into host cell E. coli HB101. Recombinant plasmid pBV220-NK was extracted for identifying by analysis of restriction enzymes, PCR and sequencing. Studies on recombinant strain's growth and expression showed that heterogene have no distinct effect on host cell. The recombinant plasmid have excellent segregational stability but low structural stability. The results of kinetics of pBV220-NK expression in E.

将重组质粒pUC19-NK上的纳豆激酶基因成功克隆到表达载体pBV220上，并将之转入大肠杆菌HB101中，得到转纳豆激酶基因工程菌，提取重组质粒经单酶切、双酶切及PCR分析验证后，对重组菌的生长及表达进行一系列研究，结果表明：外源纳豆激酶基因的导入对宿主的生长没有明显的影响：重组质粒的稳定性实验表明：该质粒具有良好的分离稳定性，而结构稳定性较差。

E studied expression levels of WAVE1 in six leukemia cell lines (the human Jurkat T leukemia cells, U937 histiocytic lymphoma cells, Burkitts lymphoma cell Raji, acute promyelocytic leukemia cell HL-60, chronic myelogenous leukemia cell K562 and K562/A02) by Western blotting analysis. Levels of WAVE1 expression were high in all six human leukemia cancer cell lines. In contrast, the constitutive expression levels of WAVE1 were noticeably lower in normal human peripheral blood mononuclear cells, or non-blood cancer cell-lines, including human lung A549 cancer cells, Hela cervical cancer cells, MG-63 osteosarcoma cells, CNE2 nasopharyngeal carcinoma cells, human umbilical vein endothelial cell, QSG7701 hepatocarcinoma cells, and WI-38 lung fibroblastoma cells.

AVE1在人类血液肿瘤细胞系(人T细胞白血病细胞系Jurkat、人组织细胞淋巴瘤细胞系U937、人Burkitts淋巴瘤细胞系Raji、人原髓细胞白血病细胞系HL-60、人慢性髓原白血病细胞系K562和K562／A02)中高表达，在非血液肿瘤细胞系(人肺腺癌细胞系A549、人宫颈癌细胞系Hela、人成骨肉瘤细胞系MG-63、人鼻咽癌细胞系CNE2、人脐静脉内皮细胞系HUVEC、人肺成纤维细胞系WI-38、人肝上皮细胞系QSG7701)以及正常人外周血单个核细胞中低表达或不表达。

PartⅡThe mechanism elucidation about effects ofα-(2,3)/(2,6)sialic acid on the Cx43gap-junction functions.(1) Westernblotting experiment showed that the decrease of sialic acid didn\'t changethe Cx43 expression and its phospholation level.(2) Westernblotting experiment showed sialidase didn,t change the ZO-1 expression,IP and confocal experiment showed sialidase improved the interaction of Cx43 andZO-1.(3) Westernblotting experiment showed sialidase didn\'t change N-cadherin expression,IP and confocal experiment showed sialidase promoted the complex formation ofCx43 and N-Cadherin.(4)Sialidase could increase the ERK1/2 phospholation level,and enchanedintercellular homotypic adhesion,Immunofluorometric assay showed sialidase couldpromote the N-cadherin cluster on the membrance.

第二部分：α—(2，3)／(2，6)唾液酸对肿瘤细胞CX43间隙连接功能影响的机制研究1、Westernblotting结果表明降低细胞膜表面唾液酸并不改变Cx43的表达及其磷酸化水平：但Cx43连接斑形成增多；2、Westernblotting结果表明唾液酸酶作用后细胞ZO—1表达没有改变，IP及免疫荧光共定位结果表明唾液酸酶作用后促进了Cx43与ZO-1的结合；3、Westernblotting结果表明唾液酸酶作用后细胞N-cadherin表达没有改变，IP及免疫荧光共定位结果表明唾液酸酶作用后促进了Cx43与N-Cadherin复合物的形成；4、唾液酸酶作用后细胞内ERK1／2磷酸化水平明显增加，细胞间同质粘附增加，以及免疫荧光表明唾液酸降低后可促进N-cadherin的膜成簇。

"Second Life is remarkably easy to work with, and is very popular,"

"第二次生命是显着容易的工作，并且很受欢迎，"

For example, we usually assume that materials are homogeneous and isotropic and free of internal defects or flaws.

为了得到适合有限元分析的模型，我们必须经过如图2所示的简化步骤。