查询词典 directed edge sequence

A method for detecting the presence of a target nucleic acid sequence in a sample, said method comprising subjecting said sample to an amplification reaction using a set of nucleotides, at least one of which is fluorescently labelled, contacting amplification product with a probe under conditions in which the probe will hybridise to said target sequence, said probe comprising a reactive molecule which is able to absorb fluorescence from or donate fluorescent energy to said fluorescent labelled nucleotide and monitoring fluorescence of said sample.

一种用于检测在某样品中是否存在目标核酸序列的方法，该方法包括：使用一套核苷酸对所说的样品进行扩增反应，其中至少一个核苷酸是被荧光标记的，在探针与目标序列杂交的条件下使扩增产物与探针混合，所说的探针包括一种活性分子，该活性分子能够从荧光标记核苷酸吸收荧光或向其贡献荧光能量，及检测样品的荧光。

According to the sequence of the hydrolase gene, we designed the primers to clone the unknown sequence by rapid amplication of cDNA ends.

根据筛选所获得的其中一个阳性克隆水解酶基因的序列，设计引物，通过cDNA末端快速扩增的方法扩增基因的未知序列。

Anti-histidine-tag antibody is usually used for identification of expression of the recombinant protein expressed using pRSET-A. A pair of primers that contained flanking hydropathic amino acid codons were synthesized, to amply the sequence coding for 10~34 aa in the precursor sequence.

质粒pRSET-A是一个常用的高效原核表达载体，编码一N端含组氨酸标签（6×His）的34aa前导肽序列，以方便利用抗组氨酸标签抗体鉴定或纯化所表达的重组蛋白。

A pair of primers that contained flanking hydropathic amino acid codons were synthesized, to amply the sequence coding for 10~34 aa in the precursor sequence. The amplified fragment was inserted into pRSET-A to generate the first double repeat of the precursor gene. By utilizing a pair of isocaudamer BamH I and Bgl II sites, and another downstream Hind III site of plasmid pRSET-A, following a series of simple double digestions and ligation of the resulted products, a series of repeat (3, 4 and 6) precursor peptide fragment genes were derived.

本实验设计一对两侧含编码疏水性氨基酸密码子的引物，经过扩增前导序列10~34aa基因序列，并重新克隆入质粒pRSET-A构建串联二聚体后，再利用质粒pRSET-A的BamH I / Bgl II同尾酶克隆位点，经一系列简单的酶切和连接，快速构建这一前导肽中不含组氨酸标签序列的串联多聚体基因，并成功表达其六聚体重组蛋白。

This thesis has made a deep study on lacustrine sequence stratigraphy and its application to oil and gas exploration and development, based upon the latest theory of sequence stratigraphy, limnology and petroleum geology incorporated with sedimentology, geophysics, tectonics, geochemistry, paleontology, paleoecology, ichnology, paleogeomagnatics and mislaneous analytical means.

"济阳坳陷下第三系沙河街组油气层序地层学研究"以层序地层学、湖泊学和石油地质学的最新理论为指导，充分利用丰富的地震、测井、岩心、录井和油藏工程等资料，综合沉积学、地球物理学、构造学、地球化学、古生物学、古生态学、遗迹学、古地磁学及多种分析测试手段对湖泊层序地层学及其在油气勘探开发中的应用进行了深入探讨。

The method applied in this paper uses the identic sequence instead of chirp sequence.

与以往采用chirp序列作为训练信号的方法不同，该文采用全序列作为训练信号。

Its website provides the introduction of this center, information of achivements, research areas, resources of genetics, related news, databases (includes GenBank Nucleotide Sequence db, DNA Data Bank of Japan, Genome Sequence db, Homologous Vertebrate Genes db, Immunogenetics db and so on).

网站提供该中心介绍，成果信息，研究领域，遗传学资源，相关新闻，数据库（包括基因银行核苷序列数据库，日本DNA资料库银行，染色体序列数据库，同源脊椎动物基因数据库，免疫遗传学数据库等等）。

Now, we can make the sequence diagram for each implemental method, if required, to describe its algorithmic sequence of signals.

现在，我们可以为每个而推行方法的序列图，如果需要的话来描述其算法的信号序列。

Base on the analysis, an improved training symbol with symmetric and repetitive structure is proposed, and the estimate range of the frequency offset is enlarged by increasing the number of repetitive sequence and shortening the interval of repetitive sequence.

在此基础上，提出了一种改进的训练符号结构，即在SC算法重复结构的训练符号中引入共轭对称结构，同时通过增加重复序列的数目，缩小重复序列之间的间隔，进一步扩大基于单个训练符号的频偏估计范围。

The increasing sequence in compliance with modular hierarchy number was speed optimizing sequence of minimum delay time, fixed resource expense and power dissipation in all cascade sequences of hybrid modules of TC2CLA.

并得出和证明了按模块层数递增级联序列是混合模块TC2CLA各序列中延迟时间最短、资源占用与功耗不变的速度优化序列。

Foods high in this vitamin include oily fish such as salmon, tuna and, and fish liver oils.

维生素D含量高的食物包括诸如大麻哈鱼、金枪鱼和鲭鱼之类的油鱼以及鱼肝油。

We need you to cosign our public letter demanding that press intimidation cease immediately, and that all charges be dropped.

我们需要您cosign我们的公共信，要求记者立即停止恐吓，并要求所有收费下降。