查询词典 clone

Swine;Chlamydophila abortus; omp1 gene;clone; prokaryotic expression

猪；流产嗜性衣原体；omp1基因；克隆；原核表达

Abortus, amplify BP26 gene by PCR and clone to pMD18-T simple vector. Identify the constructed recombinant plasmid pMDBP26 by sequencing, then subclone to vector pET-28a. Transform the constructed recombinant plasmid pETBP26 to E. coli BL21(DE3) for expression under induction of IPTG. Purify the expressed product by histidine-binding resin column chromatography and identify by Western blot.

提取布鲁氏菌基因组DNA，用PCR扩增出BP26基因，并克隆到pMD18-T载体上，测序正确后，再亚克隆至pET-28a载体上，转化到大肠杆菌BL21(DE3)中，用IPTG诱导表达，经组氨酸结合树脂柱纯化，并对纯化后的表达产物进行Western blot鉴定。

The acidophile s composition and community structure in the sediment was studied with 16S rDNA clone library and denaturing gradient gel electrophoresis.

采集了酸性矿山废水区域酸性沉积物样品，运用16S rDNA克隆文库技术和变性梯度凝胶电泳技术，研究了沉积物中的嗜酸菌类群以及它们的群落结构。

These are not aftermarket or clone bridges - they're the real thing.

这些都不是售后或克隆桥梁-他们是真实的东西。

MSCs were cultured in soft agar for 2 weeks to observe the clone growth.

结果 MSC在相应的诱导条件下可以向骨、脂肪和神经细胞分化。

This is the default clone allocator used by all pointer containers.

这是被所有指针容器使用的缺省克隆分配器。

Furthermore, the clone allocator might be allowed to have state.

此外，克隆分配器可能允许具有状态。

Please make sure you understand the Cloneable concept and the Clone Allocator concept.

请确认你已经理解了可克隆概念和克隆分配器概念。

For that reason, this library makes use of the concepts in Boost and exposes the following handy Clone Allocator classes.

出于这个原因，使得这个图书馆在提高概念的使用和公开以下方便的克隆分配器类。

Objective To clone and analyze the innate immunity-associated genes differentially expressed in Anopheles dirus.

目的 克隆及分析与先天免疫相关的大劣按蚊差异基因。

Consumer preference ; fuzzy partial ordering relation ; condorect function

消费者偏好；模糊偏序关系； condorect函数

And she doesn't have a cat, she has a Mexican hairless.

而且她不养猫，她养了一只墨西哥无毛犬。