查询词典 callus

The results showed that among three regulator combinations (2, 4-D KT and IBA), 2, 4-D2~3 mgL^(-1 treatment was the best with the highest callus induction rate, most of the explants induced callus and grew well. 2, 4-D2mgL^(-1+KT0.5mgL^(-1 treatment could induce callus from stem parts while the callus induced from the stem top was water-soaked and easy to be brown stained. KT4mgL^(-1 treatment could induce callus on some stems but was easy to be brown stained. KT4mgL^(-+IBA0.5mgL^(-1 treatment could induce callus but they grew very slowly.

试验结果表明：在3种生长调节剂(2， 4-D、KT、IBA)的组合中，以2，4-D2~3mgL^(-1效果最好，愈伤组织的诱导率最高，大部分外植体都能诱导出愈伤组织，生长良好；2，4-D 2mgL^(-1)+KT0.5mgL^(-1)能使部分茎段诱导出愈伤组织，而茎尖诱导的愈伤组织水渍化，易褐变；KT 4mgL^(-1)能使个别茎段有愈伤组织产生，但易褐化；KT 4mgL^(-1)+IBA 0.5mgL^(-1)能诱导出愈伤组织，但生长缓慢。

Three kinds of explants from Cajanus cajan were used to established callus culture. Ethanolic extract prepared from five plant materials, callus from cotyledon, callus from hypocotyl, callus from root, Cajanus cajan seeds and Glycine max seeds were examined for their phenolic contents, flavonoids contents and DPPH radical - scavenging activity.

摘要 本研究建立培养树豆三种部位愈合组织，以总酚含量、总黄酮含量及DPPH自由基清除能力三种方式分析其抗氧化能力，并与成熟的树豆及黄豆种子比较；再利用genistein和daidzein两种标准品，检测这五种检品中大豆异黄酮的含量。

Content of flavone is the hightest in browning callus. It is supposed, that suppose which the last pace of synthesize flavone is in brovwing callus. SDS-PAGE cataphoresis electrophoresis show protein has big change from differentiating callus to browning callus. The change may has relation with change of enzymire activiation of control flavone synthesize.

黄酮含量的变化以褐化愈伤组织中含量最高，因此我们推测：黄酮合成的最后几步，可能发生在褐化愈伤组织中，从可溶性蛋白质的 SDS-PAGE 平板电泳结果表明，分化愈伤组织与褐化愈伤组织相比蛋白质谱带发生了明显的变化，这可能与控制黄酮合成的有关酶的活性变化有关。

Result: The inductivity of leaves was the highest about 87.5%, followed with the stem section and leafstalk; The inductivity of nutrient medium such as MS, B5 callus was higher than the ones like H, SH and the White callus amended one; It was found that low-grade Phvalue benefits the growth of callus. The experiment result showed that different pH showed little difference in quality. The best condition of culture was 25 ℃in temperature. Conclusion: The best culture condition for callus was the leaves as explantation.

结果：叶片的诱导率最高，为87.5%；茎段次之，叶柄较差；培养基MS，B5愈伤组织诱导率较高，达到了80%以上，高于培养基H，SH和改良White的愈伤组织诱导率；偏低的pH对愈伤组织生长有利，出愈率高，但从愈伤组织生长质量方面看，不同pH差异不明显；愈伤组织诱导最适温度为25 ℃。

B Of the six basic media MS, MS1/2 (half-strength of MS salts and vitamins), WPM, DKW, B5 and SH, MS1/2 was the most proper one to induce somatic embryos. Somatic embryos generally regenerated directly from excised zygotic cotyledons. PGRs combination affected somatic embryogenesis significantly. Medium with NAA 1mg/L, TDZ 0.05mg/L, IBA 2—10mg/L combined with BA 10mg/L, or IBA 10mg/L integrated with BA 0-2mg/L gave the highest induction rate. Excised zygotic hypocotyls had the strongest potential to produce callus. Callus induction was also affected significantly by media and PGRs. The proper callus induction condition was MS1/2 medium containing NAA 1mg/L, IBA 10mg/L, BA 2-5mg/L and TDZ 0.05mg/L. Harvest period affect somatic embryogenesis significantly. Zygotic embryo explants collected from the end of July to the middle of August had strong potential to generate somatic embryos, when endosperm finished solidification, different parts of the embryos were completely formed, the size of embryos occupied about 2/3 of the embryo sac. Provided with optimized conditions, direct somatic embryogenesis rate can attain to 33. 68%, and callus induction rate of hypocotyls was up to 90.7%. Cytological observation on megasporogenesis and zygotic embryogenesis of Manchurian ash showed that the ovary was twicarpellum, twilocular with two ovules each loculus. The ovule was tenuinucellar and anatropous, with one megasporcocyte. The development of embryo sac is of the Polygoum type.

体细胞胚胎发生研究的结果表明：(1)成熟过程中的合子胚是诱导水曲柳体细胞胚胎发生的最佳外植体材料；(2)在所试验到的MS、MS1/2（将MS的所有成分均减半）、WPM、DKW、B〓、SH等六种基本培养基中，MS1/2是最适合诱导水曲柳体细胞胚胎发生的基本培养基；(3)水曲柳的体细胞胚胎发生以直接发生为主，体细胞胚主要来自于从合子胚分离的完整子叶；(4)培养基中的激素组合对水曲柳的体细胞胚胎发生有显著影响，诱导直接体细胞胚发生较好的激素组合有NAA 1mg/L+IBA 2，5，10mg/L+BA 10mg/L+TDZ 0.05mg/L和NAA 1mg/L+IBA 10mg/L+BA 0，2mg/L+TDZ 0.05mg/L；(5)合子胚分离的下胚轴具有最强的愈伤组织诱导潜力，少数愈伤组织可以分化出体细胞胚；(6)愈伤组织的诱导也受培养基和激素配比的显著影响，最适宜诱导的培养条件为MS1/2+NAA 1mg/L+IBA 10mg/L+BA 2，5mg/L+TDZ0.05mg/L；(7)采种时间对体细胞胚胎发生有显著影响。7月末到8月中旬的合子胚具有较强的体细胞胚发生潜力，此时种子尚未成熟，胚乳已呈固态，种胚的各个部分已分化完全，种胚体积占胚腔的大约2/3；(8)在各自综合的最适条件下，完整子叶的体细胞胚诱导率可达33.68%，下胚轴的愈伤组织诱导率可达90.7%。

Culture tender leaves in culture medium of MS+2,4-D1.5mg/L+30g/L suger+0.7% agar pH5.8 for 20 days in darkness at 25℃, and then subculture to induced Ⅱ-type calli. Use forceps cutting the tissue to nubble with 2mm2, and put the tissue into Agrodacterium tumefaciens LBA4404 liquid supplemented with AS 100mg/L,then, co-culture 3 days, resume 7 days in MS+2,4-D1.5mg/L+30g/L suger+500mg/L cef, take to MS+2,4-D1.5mg/L+30g/L suger+100mg/L cef+10.0mg/L kanamycin culture 20 days in darkness. After that to make it polarize in MS+30g/L suger+100mg/L cef+10.0mg/L km. The percentage of km resistant callus was reached max after infection for 45 min, the average is 29.66%. Simultaneity, tender leave callus are infected 5 min by A. tumefaciens liquid in different negative pressure, and kept on 15 min in Agrodacterium tumefaciens liquid without negative pressure. Then screen out resistant callus and obtain transgenic plants. When the negative pressure is -0.05MP the percentage of km resistant callus was reached max, the average rate is 37.5%.

将心叶接种于MS+2.4-D1.5mg/L+30g/L 蔗糖，琼脂0.7%，pH5.8 培养基中25℃暗培养20d 后继代一次，诱导产生Ⅱ型胚性愈伤组织，用镊子将其夹碎成2mm2大小的小块，置入添加100mg/L AS 的根癌农杆菌LBA4404 菌液中，侵染时间为45min，共培养3 天后，转入MS+2.4-D1.5mg/L+30g/L 蔗糖+500mg/L Cef 培养基中恢复7天，再转入MS+2.4-D1.5mg/L+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 中，遮光培养20d后，置入其MS+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 分化，卡那霉素抗性愈伤组织获得率在侵染45min 时达到最大值平均为29.66%；同时以甘蔗心叶愈伤组织材料，通过循环水式真空泵，产生负压，设定不同的负压值，在农杆菌菌液中感染5min 后，在负压条件下继续侵染15min 后，筛选出抗性愈伤组织并获得转化植株，其中在负压为-0.05 时转化率达到最大值，其卡那霉素抗性愈伤组织获得率平均为37.5%。

The normal and abnormal embryoid were distinguished by the rate of normal plantlets induced and the regeneration modes of the cottons varieties were compared to two ways, one with the highest rate of normal inducement was the normal way of regeneration plantlet from callus, embryogenetic callus, global embryoid, heart-shape embryoid, torpedo-shape embryoid, cotyledon embryoid to normal plantlet, etc, the other with the lowest rate of normal inducement was the abnormal way from callus, embryoid callus, global embryoid-like, heart-shape embryoid-like, fish torpedo-shape embryoid-like, cotyledon embryoid-like, abnormal plantlet to normal plantlet, etc.

转化后采用平行实验得到不同品种的不同培养体系的愈伤组织、胚性愈伤组织、胚状体、类胚状体、畸形苗和正常苗，以最终正常苗诱导率高的体系的材料作为正常苗的发生过程，以诱导率低的作为畸形苗的发生过程，即分为正常苗的发生过程，愈伤组织——球型胚状体——心型胚状体——鱼雷型胚状体阶段——子叶型胚状体——正常苗：畸形茁的发生过程，愈伤组织——类球型胚状体——类心型胚状体——类鱼雷型胚状体阶段——类子叶型胚状体——畸形苗。

The results showed as following: NMB was suitable for the callus induction, and MS for differentiation.500 mg/L of Glu or Arg redounded to the callus induction.All the cultures were kept under dark, 25-28 ℃, pH 5.8 for callus induction; and 25-28 ℃, 1 500-2 000 lx,12-14 h/d,pH 5.8 for differentiation.6-BA shows a significant result on the growth of callus;The optimal concentration of hygromycin is 50 mg/L.

结果表明：NMB培养基适合水稻成熟胚盾片愈伤组织诱导，MS培养基适合愈伤分化；500 mg/L谷氨酰胺或精氨酸有利于愈伤组织的形成；培养条件：愈伤组织诱导时温度25～28 ℃，暗培养，pH 5.8；分化时温度25～28 ℃，光照1 500～2 000 lx，12～14 h/d，pH 5.8；6-BA对继代过程中愈伤组织的生长有促进作用；潮霉素筛选的最佳浓度为50 mg/L。

The results indicated:0.1 mg/L 2,4-D or 0.1 mg/L 2,4-D+0.2 mg/L KT was optimal to induct the callus of carrot hypocotyls.The majority of the callus induced on the medium containing 0.1 mg/L 2,4-D regenerated embryoid,whereas,the callus induced on the medium containing 0.1 mg/L (2,4-D)+0.2 mg/L KT produced adventive buds.During the growth of regenerated plant,MS medium is available for rooting of seedling,and seedling regenerated on B5 medium must be placed on the B5 medium containing 0.1% IBA to develop root.

试验结果表明：0 1mg/L2，4 D或0 1mg/L2，4 D+0 2mg/LKT的激素组合有利于胡萝卜下胚轴的愈伤组织诱导；在0 1mg/L2，4 D的培养基上形成的愈伤组织主要以胚状体的形式再生，而在含0 1mg/L2，4 D+0 2mg/LKT激素组合的培养基上形成的愈伤组织主要以发生不定芽的方式再生；再生过程中，B5基本培养基上的苗子不易生根，需要附加0 1mg/L的IBA，而在MS培养基上苗子可直接形成根。

In the present experiment, the tender stems of Dracaena cambodiana were used as explants to establish a two-step tissue culture and regeneration system. The effects of types and concentrations of plant hormones on the induction of callus were studied. Based on these, the reactions of callus regeneration to different mutation-induced approaches (60Co irradiation, EMS and colchicines), the regeneration ability of the treated callus as well as the characters diversities (including the appearance traits and cytological observations) of the regeneration plants were investigated.

本试验以龙血树幼嫩茎段为材料，通过对龙血树幼嫩茎段两步培养法再生的研究，建立了龙血树幼嫩茎段离体再生体系，证明了愈伤组织的发生和激素种类与浓度的关系；并在此基础上研究了愈伤组织再生对不同人工诱变方法（60Co辐射、EMS、秋水仙素）的反应、被处理愈伤组织再生能力和再生植株性状多样性、以及再生植株不同生理角度的变异性，得到了2株变异株系。

So it was last week in Selma , alabama.

上星期在亚拉巴马州塞尔马市发生的事件也是这样一个转折点。

The group also spearheads various social programs in China.

该组织还带头参与中国的各种社会活动。