查询词典 callus

Results For callus induction of leaf explants of C. orchioides, dark treatment gave better results compared to light treatment; among the media tested, the suitable phytohormone combinations were 2.0 mg/L 2, 4-D or 6-BA 1.5 mg/L 2, 4-D 2.5 mg/L, and 300 mg/L CH 0.2% AC was good for plantlet regeneration from leaf explants. The callus from leaf explants mainly originated from midrib. The parenchyma cells near epicuticle of midrib firstly were initiated to division. Then the parenchyma cells of vascular bundle sheath and mesophyll cells on each side of vascular bundle were also divided to form callus.

结果对仙茅叶片的愈伤诱导，黑暗的效果好于光照；在所试验的培养基成分中，适宜的激素配比是2.0mg/L2，4-D或6-BA1.5mg/L 2，4-D2.5mg/L，并且附加300mg/L水解酪蛋白和0.2%活性炭，对于仙茅叶片的离体成苗较好；培养后，愈伤组织主要由叶片的中脉产生，位于中脉上表皮内侧的薄壁细胞首先启动分裂，随后维管束鞘薄壁细胞及其外侧的叶肉细胞也启动分裂，参与愈伤组织的形成。

The callus induction of licorices was easy. And the MS+1.0 mg/L NAA+1.0 mg/L 2, 4-D +1.0 mg/L 6-BA was the best medium to cotyledons, hypocotyls and radicles. And all the induction rates were 100%. 4. In the study of callus regeneration, only obtain the regeneration plants of hypocotyls callus,and its frequency was 4.57%.

甘草离体再生中愈伤组织的诱导较为容易，三种外植体子叶、下胚轴和胚根均以MS+1.0 mg/L NAA+1.0 mg/L 2，4-D+1.0 mg/L 6-BA为愈伤组织诱导的最佳培养基，在此培养基上，3种外植体愈伤组织诱导率均达到100%。

L^(-1), the growth of callus was inhibited. However, the effect of Carbenicillin on callus was little and the differentiation of adventive buds was promoted under appropriate concentration. When the Carbenicillin's concentration was 200mg?

L^(-1)时，愈伤组织生长受到抑制；而羧苄青霉素对愈伤组织影响很小，在适宜的浓度下能促进不定芽分化，213品种在羧苄青霉素浓度为200mg？

The higher the concentration of Cefotaxime was, the lower the rate of differentiation was; when the concentration of Cefotaxime was high 400~500mgL^(-1, the growth of callus was inhibited. However, the effect of Carbenicillin on callus was little and the differentiation of adventive buds was promoted under appropriate concentration. When the Carbenicillin's concentration was 200mgL^(-1), the differentiation rate reached the maximum 68.8%.

头孢霉素浓度愈大，213品种不定芽分化频率愈低，当头孢霉素浓度较高400~500mgL^(-1时，愈伤组织生长受到抑制；而羧苄青霉素对愈伤组织影响很小，在适宜的浓度下能促进不定芽分化，213品种在羧苄青霉素浓度为200mgL^(-1)时，分化频率达到最大值68.8%。

The plant expression vectors were constructed and two genes were transferred into A. thaliana and N. tabacum, antikanamycin plants were obtained.4. The trials of adventive bud regeneration directly and callus induction was conducted to select out the optimal explants and formula. The results revealed that double scale can produce 13~16 adventive buds per centimeter in MS medium added 10.0 mg/L 6-BA, 1.0 mg/ L NAA, 40 g/L sugar and 7.5 g/L agar. Scale, leaves, scape and different tissue of flower can be induced to produce callus in appropriate medium, but the filament with anther was the optimized explant.

构建了两个基因的pBI121和pCAMBIA1300植物表达载体，并通过农杆菌将上述基因转入烟草和拟南芥中，得到了卡那抗性的再生植株。4、以中国水仙不同部位外植体进行了不定芽直接分化和愈伤组织诱导分化试验，结果表明，双鳞片外植体在MS0+6-BA 10.0 mg/L+NAA 1.0 mg/L+蔗糖40 g/L+琼脂7.5 g/L培养基上可以大量地直接再生，再生芽数量平均可达13~16个/cm双鳞片，比传统的鳞茎盘之间分化提高30~50%；鳞片、叶片、花葶及花部不同组织在适宜的配方中均可不同程度地诱导出愈伤组织，但以带花药的花丝诱导出的愈伤组织最好。

At day 3, 6, 9, 14, 28, 42 and 56, five rabbits were selected from every group randomly and were killed by aeroembolism respectively. Their radius were taken out and the left one was taken as the research object. Immunohistochemistry stain and in situ hybridization stain were performed to examinate the VEGF and VEGF mRNA expression in the haematoma, fibrous callus and soft callus.

分别于术后3，6，9，14，28，42，56 d每组随机处死5只家兔，取左侧桡骨标本作为观察对象，通过免疫组化和原位杂交方法检查骨折端血肿、纤维骨痂和软骨骨痂中VEGF及VEGF mRNA表达。

The karyotypes and the Giemsa-C banding patterns of Aegilops tauschii (85A-34) and Hordium vulgare (H2) and regenerated plants from embryo callus of hybrids between Ae.tauschii and H.vulgare (2n=28) were confirmed by karyotype analyzing and Giemsa-C banding technique in this study, thus proved that the regenerated plants from embryo callus of hybrids between Ae.tauschii and H.vulgare (2n=28) were spontaneously chromosal doubling amphidiploid.

本研究通过染色体组型分析和Giemsa--C带显带技术，确定了节节麦（85A85A-34）、栽培大麦（H2）、节节麦×大麦杂种胚再生植株(2n=28)的染色体组型和Giemsa--C带核型，证明了节节麦×大麦杂种胚再生植株(2n=28)为自发加倍的节节麦－大麦双二倍体。

It was observed that the influence of bud and its position on the healing process of grafting unions using hardwood sectioning method, and found that:(1) bud of a scion and its position to the graft unions have no influence on callusing formation of the isolation layers, and the early differentiation of cambia;(2) a scion bud closer to the grafting union would promote the formation of more compacted callus cells and the earlier formation of callus bridges;(3) conduit differentiation in grafting unions of lower-bud scions was later than that of other treatments.

利用滑动切片法对芽子有无及芽位对嫁接愈合过程的影响进行了观察，发现：(1)接芽有无及芽位对隔离层及愈伤组织形成和形成层的早期分化没有影响；(2)芽或去芽的芽位距接口近，可以促进愈伤组织的紧密连接，即愈伤组织桥的形成；(3)低位芽枝嫁接体中导管分化较晚，而其它处理导管的分化几乎是同时发生的，而且导管首先出现于愈伤组织薄壁细胞中。

In this paper, induction of callus and regeneration of plants from cuttingpiece of bulb and scalybud-top in fritillaria pallidiflorae were studed, whichhas indicated that in case of adding 2,4-D 1mg/1 and KT 0.1 mg/1, thecallus was induced on the MS medium; in cuse of adding IAA 1 mg/1, NAA0.2 mg/1 and KT 0.1 mg/1, the bulblet from both callus was differentiated.

在加有IAA1毫克/升和NAA0.2毫克/升及KT0.1毫克/升的MS培养基上分化出了小鳞茎。

When cultivated in medium with a certain level of antibiotic, the percentage of callus inducing of cotyledon burl is always the same, while the percentage of callus inducing of leafsteak is different.

在一定浓度抗生素的培养基中，子叶节的愈伤组织出愈率均为100%，而叶柄的出愈率在不同的浓度下会有明显的不同。

The teacher likes the honeymouthed little girl very much.

老师很喜欢这个嘴甜的小姑娘。

Mr. Notker Bien's interests are traveling, spending quality time with the family and long-distance-running.

诺特卡·柏恩先生热爱旅游，长跑，以及和家人一起共度美好时光。