查询词典 callus

This paper presents a study of Eucalyptus dunnii tissue culture, using its seeds as explants. The results reveal that MS medium is a suitable basic medium for its seeds to germinate and grow; that MS+KT1.0 mg/L+2, 4-D2.0 mg/L+Homocysteine30mg/L is a good medium to induce its seeds to dedifferentiate directly into callus; that H+6-BA2.0 mg/L+IAA0.2 mg/L is a good medium to make its bud on the seedling reproduce more buds; that MS+6-BA2.0 mg/L+NAA0.2 mg/L is a better medium to induce its root on the seedling into callus; that B5+6-BA2.0 mg/L+2, 4-D0.2 mg/L can induce its under-hypocotyl to differentiate into buds; and that B5+Ad2.0 mg/L+IAA0.2 mg/L can induce its under-hypocotyl into callus to generate normal buds.

以邓恩桉种子为外植体，探讨用最少种子快速繁殖最多幼苗的方法，结果表明：MS培养基是较适合邓恩桉种子萌芽和生长的基本培养基；诱导种子直接脱分化成愈伤组织的较佳培养基配方为MS+KT1.0 mg/L+2，4-D2.0 mg/L+半胱氨酸30 mg/L；以邓恩桉实生苗的芽来繁芽的较理想培养基配方为H+BA2.0 mg/L+IAA0.2 mg/L；邓恩桉实生苗的根诱导愈伤组织的较佳培养基配方为MS+6-BA2.0 mg/L+NAA0.2 mg/L；诱导邓恩桉下胚轴分化芽较佳培养基配方为B5+6-BA2.0 mg/L+2，4-D0.2 mg/L；诱导邓恩桉下胚轴脱分化为胚性愈伤组织的较佳培养基配方为B5+Ad2.0 mg/L+IAA0.2 mg/L。

High content of ABA was negative to callus induction, but might increase embryogenic callus in the process of callus regeneration.

内源激素对愈伤组织的生理状态有着极其重要的影响，其种类、含量及协调平衡是水稻愈伤组织形成和分化的关键。

Results (1) Primary callus came into being 3 days after operation. Mesenchymal cells from peripheral tissues around the primary callus were differentiated into chondrocytes 1 week later. Endochondral ossification was going on then. Bridging callus was formed in the fracture sites 4 weeks after operation.

结果 (1)伤后3 d开始形成原始骨痂。1周时肉芽组织中的间质细胞开始分化为软骨细胞，软骨形成后再进行软骨内化骨。4周时形成连接骨折端的桥接骨痂。

The invention clones geranyl pyrophosphate synthase GGPPS gene from the ginkgo to construct plant expression vectors which contain ggpps gene, the ggpps gene is inducted into immature embryos of the ginkgo to induct out the callus tissue by the mediating of agrobacterium tumefaciens, PCR and semiquantitative RT-PCR detect the conformation and expression status of exogenous target gene ggpps, high-performance liquid chromatography and an evaporative light-scattering detector are employed to determine the terpene lactones content inside the callus tissue of the ginkgo, and the obtained callus tissue of the ginkgo of which the terpene lactones content is increased is screened.

本发明从银杏中克隆香叶基香叶基焦磷酸合成酶GGPPS基因，构建含ggpps基因的植物表达载体，用根癌农杆菌介导，将ggpps基因导入银杏幼胚并诱导出愈伤组织，PCR和半定量RT-PCR检测外源目的基因ggpps的整合和表达情况，高效液相色谱法及蒸发光散射检测器测定银杏愈伤组织中萜内酯含量，筛选获得银杏萜内酯含量提高的转基因银杏愈伤组织。

Don.methodswith the stem of sterile seedling and fresh plants as exophyte, ms as a basic medium, 6-ba and naa hormone were adopted as the experimental variables.resultsms +1.0 mg / l 6-ba +1.0 mg / l naa induced the best effect of callus; higher concentrations of 6-ba with high concentrations of naa made callus densification and higher rate.conclusion different plant hormones and their different concentration ratios have different effects on the callus of halenia elliptica d.

方法以椭圆叶花锚种子培养的无菌苗的茎和新鲜植株的茎段为外殖体，ms为基本培养基，分别以6-ba和naa两种植物激素为变量进行实验研究。结果ms培养基+1.0 mg/l 6-ba+1.0 mg/l naa对诱导椭圆叶花锚愈伤组织的形成效果最佳；高浓度的6-ba配合较高浓度的naa诱导的愈伤组织致密，出愈率高。

Cotyledon and hypocotyl ' s rate and quamity are the most among these explams , and callus can be obtained in 10 days by cotyledon and hypocotyl . reversely it is difficult to indue callus with root , and the callus from root is lnde and easy to become browning . the calius obtained from leaf grows very slow and does not become browning uniill in 2 or 3 months

银杏的不同器官和组织都能够诱导出愈伤组织来，其中，子叶和胚轴10d左右全部愈伤化，诱导速度和诱导率均最高，根则很难诱导，愈伤组织很少，褐化很快；叶片诱导的愈伤组织，生长慢，褐化也慢，在培养基上保持两三个月而不褐化；胚乳的诱导时间也较长，需要30d左右。

Firstly, callus induction and subculture for Mikania micrantha were studied systematically. The research on callus induction showed that: Shoot tip has higher induction rate than other explants ; At 25℃ and dark regime, the callus was better cultivated on Murashige and Skoog medium (pH5.8) containing 2.0mg/L 2,4-D and 1.0mg/L KT.

首先，对微甘菊愈伤组织诱导和继代增殖培养进行系统研究，愈伤组织诱导研究表明：外植体中芽尖诱导率最高，在含2，4-D2.0mg／L、KT1.0mg／L、pH5.8的MS培养基上25℃黑暗条件下愈伤组织诱导效果较好。

The experiment result showed: when the medium was MS supplemented with 2,4-D2.0mg/L, BAl.0mg/L and NAA0.5mg/L, the callus induction rate of the cotyledon was 87.9%, the callus induction rate of the plumular axis was 82.9%, and the callus induction rate of the terminal bud was 6.7%.

实验结果表明在MS附加2，4-D2.0mg/L、BA1.0mg/L、NAA0.5mg/L的培养基上，子叶愈伤组织的诱导率为87.9％、胚轴的诱导率为82.9％、顶芽的诱导率为6.7％。

The results demonstrated that, by comparison with un-transgenic Roselle cell or callus, the critical plating cell density and critical initiating cell density of transgenic cell line were cut down 60% and 50%, respectively,the growth cycle of transgenic cell in suspension culture was shortened from 16 days to 12 days, and the specific growth rate of transgenic Roselle callus was raised 75%, the content of flavonoid compounds in transgenic Roselle cell line or callus was hardly altered.

研究结果表明，所获得的转基因玫瑰茄细胞系的临界植板细胞密度和临界起始细胞密度分别降低了60%和50%，悬浮细胞培养周期由16d缩短到12d，转化愈伤组织的比生长速率比对照提高了75%，转基因玫瑰茄细胞和愈伤组织中的花黄素含量与对照相比没有发生明显变化。

Histochemically, the results indicated that POD activity increased with the growth rate of callus within the same tissue; and lignin deposit density within the cell wall increased with the growth of callus over time, displaying a trend similar to POD activity alteration upon Hg(superscript 2+) imposition; Furthermore, more tracheary elements was observed in the callus tissue subjected to Hg(superscript 2+) than that in control.

组织化学定位结果表明：同一处理样品的过氧化物酶活性随着愈伤组织的生长进程而逐渐增强，且与Hg(上标 2+)浓度呈正相关；细胞壁中木质素沉积随着愈伤组织的生长而增加，与过氧化物酶活性变化趋势一致；Hg(上标 2+)处理的愈伤组织中导管诱导率大于对照。

Continuously improve the production mechanism, to provide customers with better quality products and services.

不断的完善生产机制，给客户提供更优质的产品及服务。

B:Yes, he was the very prize-winner in the court tennis ten years ago?

是的，他是十年前的世界网球冠军。