查询词典 callus

The invention provides a method for producing transgene sugarcane quickly with pmi genes. The method primarily comprises the steps of establishing a mannitose screening system; transformation of a gene gun; acquisition of resistant callus; differentiation and screening of the resistant callus; acquisition and rootage of resistant plantlets; molecule detection of resistant plantlets regenerated and detection of pmi gene expression by the chlorophenol red detection method.

一种利用pmi基因快速获得转基因甘蔗的方法，主要内容包括：甘露糖筛选体系的建立，基因枪转化和抗性愈伤组织的获得，抗性愈伤组织的分化筛选，抗性苗的获得和生根，抗性再生苗的分子检测和氯酚红法检测法检测pmi基因表达。

Result The corm and scale were easy to induce the callus on the medium of MS+1. 0 mg/L 6-BA+1.0 mg/L NAA, with the induction of 92% and 90% resp. and the induced callus was easy to differentiate.

结果］球茎和鳞片在MS+6-BA1.0 mg/L+NAA1.0 mg/L培养基上容易诱导愈伤组织，诱导效率分别为92%和90%，且愈伤组织容易分化。

It is vital to reduce the variation of Dracaena fragrans cv.massangeana and Dracaena fragrans cv.lindenii by preventing the differentiation from forming callus as well as the reduction or the prevention of callus redifferentiation.

避免脱分化形成愈伤组织以及减少或阻止愈伤组织再分化是减少金心也门铁和金边也门铁这类嵌合体品种发生变异的关键之一。

Furthermore, supplements of callus induction medium under N6 basic medium,such as concentration of 2,4-D, proline and casein hydrolysate were used to study their effects on the induction frequency,embryogeny and quality of callus,the inhibition of embryo spontaneous shooting.

幼胚在附加4 mg/L 2，4-D且去掉水解酪蛋白和L-脯氨酸的N6改良培养基上，幼芽及根的数量少、出愈率高、愈伤组织质地好，并明显减少了幼胚自发性芽分化的趋势，有利于保持愈伤组织胚性、提高再生植株频率，为玉米自交系幼胚诱导的适宜培养基。

Results The inductivity of stem was the highest about 55.56%,but callus of leaves could not be induced.6-BA was the most important factor on callus induction,followed by 2,4-D NAA and sucrose.

结果茎段的诱导率最高，为55.56%，叶片诱导不出愈伤组织。6-BA是叶下珠愈伤组织形成的主要影响因素，2，4-D、NAA、蔗糖次之。

This paper reports something about it .The callus was induced and proliferated by using young stem , leaf and leafstalk as explants .which came out in early spring . In this paper , its effects on induction and proliferation were discussed by using different kinds of explants and hormone and different physical and chemical factors.The growth circle and some physiological and biochemical factors were studied during the culture of callus, a set of rapid propagation techniques were founded, which was rapid, efficient and steady.

本文以丹参初春萌发的幼茎、叶、叶柄为外植体，探讨了不同的外植体、不同的植物生长调节物质、不同的理化因子对愈伤组织的诱导和增殖效应，研究了黑暗和光照条件下愈伤组织细胞培养生长周期以及生长周期中生理生化指标的变化特征，摸索了一套快速、高效、稳定的丹参快速繁殖技术，为丹参组织培养、次生代谢物的生产提供理论依据，为丹参快速繁殖应用于生产提供技术支持。

The major results as the following: Medium MS+6-BA 2.0 mg·L-1 +2,4-D 0.5 mg·L-1 is optimum for callus induction of C.japonica "Naidong";The pH 5.5 is comparatively suitable for C.japonica "Naidong" callus induction;Using the leafstalk of C.japonica "Naidong" as the exp...

研究结果表明：MS+6-BA 2.0 mg.L-1+2，4-D 0.5 mg.L-1培养基是耐冬山茶愈伤组织诱导的最佳培养基；耐冬山茶愈伤组织诱导培养基pH值以5.5较为合适；耐冬山茶叶柄作为外植体，诱导率和生长状况最佳。

Mixed anther of seven alfalfa varieties or Strains (MT-2, MT-146, MT-328, MT-335, Xinmu No.1, Xinjiang Daye and KS220-97) were inoculated on the double layer medium which contained N6 macroelement, MS microelements, MS molysite, B5 organic compound, 2,4-D 2 mg/L, 6-BA 0.5 mg/L, and induced callus, then the callus was induced on the medium of the different composition and the additives, to observe its result of culture.

将7种苜蓿品种（MT-2、MT-146、MT-328、MT-335、新牧1号、新疆大叶和KS220-97）的混合花药接种于N6大量元素＋MS微量元素＋MS铁盐＋B5有机物＋2，4-D 2mg/L+6-BA 0.5 mg/L的固液双层培养基上，诱导出愈伤组织，再转接到不同成分及添加物的培养基中培养，观察培养效果。

Of the different types of callus induced, type III callus was selected for morphogenesis induction.

所诱导的愈伤组织类型各异，其中Ⅲ型愈伤组织用於形态发生的建立。

The results showed that PYM could dramatically inhibit callus induction and differentiation of explants. With the increasing of PYM concentration, hoth the callus induction rate and differentiation rate decreased.

结果表明，PYM对叶片和茎段愈伤组织的诱导和分化具有明显的抑制作用，随着PYM质量浓度的增加，愈伤组织的诱导和分化均呈明显的下降趋势。

It was just a normal day of school

那只是上学的普通一天

Which is a very good reference in this issue.

这是一个在这个问题上很好的参考。