查询词典 and then

Methods: One-step trizol had used to get the total RNA of the four animal brain, then PEGS000 to purify the small RNA from the total RNA, after the ligation ofpoly on the 3\' and the adaptor on the 5\', the oligo primer will be used to reverse translation the small RNA, and then used the specific primers to amplification the small RNA sequences. After the gel purition recovery, the small RNA will be ligated to the T-vector, and be transformed to the TOP 10 E. coli, at last, we picked up the masc. clones for sequencing.

通过Trizol一步法提取四种动物脑中的总RNA，接着用PEG纯化其中的小分子RNA，再经过poly连尾反应在小RNA的3'端连接上一段poly，然后在5'端连上adaptor，用Oligoprimer将连接好的小RNA进行反转录，用根据adaptor和Oligoprimer设计的引物来扩增小RNA，扩增后的小RNA进行胶回收后连接到T载体上，转化TOP10大肠杆菌后挑取阳性克隆进行测序，得到的序列再经过生物信息学的分析后确定是否为miRNA。

And then I'll do some mockups and then I take it over to Julian and hope he can make those mockups look far better than my mockups.

之后我就做一些模型并交给Julian，然后希望他能将这些模型更加完善。

And then I'll do some mockups and then I take it over to Julian and hope he can make those mockups look far better than my mockups.

之后我会大概做个样子出来，然后我会把那东西拿去给Julian看，然后希望他会把那些草稿真正弄出个样子来。

Selected material, to sawn ivory films, shape file, and then polished with sandpaper 6 times, and then the surface of ivory black ink will be dry after.

选定材料后，要把象牙锯成片、锉成形，然后用砂纸打磨6遍，再用墨汁将象牙表面涂黑后晾干。

Ace hardware is actually to the company, then the company has entered into a phototypesetting field, and then joined the ranks of the digital, the company is always trying to make myself followed by technology and market development.

Ace 公司实际上是以硬件起家的，后来公司进入了照排领域，而后又加入了数字化行列，公司总是试图让自己紧跟技术和市场的发展。

What a coincidence! Or is it? Whenever we have a ?spooky? or uncanny moment when something happens that gives us goose pimples we know we are experiencing serendipity. Thinking of your mother and then she calls you? Talking about someone and then they show up? Happens a lot doesn?t it?

好像最近每天写英文，说英文的时间要比用别的语言多很多，年终岁尾，感谢信、贺卡之类的东西，都是要中文下面加英文翻译，否则等于没写，一年也就这么几天，把已经发霉的英文，拿出来晒晒。

Language qua said can then be conceived as a system of nouns identifying entities, and then as a system of signs doubling up the beings, designating substances, events and relations by substantives or other parts of speech derived from substantives, designating identities---in sum, designating .

语言作为所说于是能被构想为一个标识实体的名词系统，而且一个双重化存在者，标示物质、事件以及通过实词或来源于实词的其他词类的关系，指定身份的符号系统——总而言之，标示。

When your dog gets used to doing this command try going in circles, in figure of eight, and changing directions suddenly, if the command is still working then try your dog on short walks and then build your way up to longer walks as you progress.

当你的狗被用来做这个命令尝试去圈子，数字8 ，并突然改变方向，如果该命令仍然是工作然后再尝试您的狗在短期散步，然后建立自己的方式行动不再走你的进展。

This method changes the generation process of the constrained texture synthesis, first of all it synthesises textures, and then constraints regionals, it makes the binding process easy and separate the synthesis process, overcomes the constraints of regional boundaries and repeated to determine synthesis methods used interchangeably cumbersome, then speeds the process.

该方法改变了以往的生成过程，首先进行纹理合成，再进行区域约束，使得约束过程与合成过程分开，从而减弱了约束边界在多样图纹理合成时对合成算法的约束，克服了反复判断区域边界的繁琐，进而加快了合成速度。

"No, no, I'll take care of that myself," Joe snickered evilly, then his face turned dour again, And if you tell anybody -- ANYBODY -- about why I didn't get up in front of them, I'll grind your corpse into hamburger, sell you to McDonald's and THEN tapdance on you!

& / &不，不，我将照顾那我自己&，乔邪恶地吃吃地笑，然后他的脸再一次变不爱讲话的&，而且如果你告诉任何人--任何人--关於我为什麼在他们之前没有起床，我将磨你的尸体进汉堡之内，卖你给麦当劳公司然后在你上的 tapdance！

If you are unfortunate enough to the lovelorn, please tell me, I will help you out, really, please contact me!

如果你不幸失恋了，请告诉我，我会帮助你摆脱困境，真的，请联系我啦！

China's plan to cut energy intensity by 20 percent and pollutant discharges by 10 percent between 2006 and 2010 is a case in point.

中国计划在2006年到2010间降低20%的能源强度和减少10%的主要污染物排放，就是一个这样的例子。