查询词典 amplification

The Gordonia specific 16S rDNA fragment (829 bp or 436 bp) was successfully amplified for all the reference Gordonia species with the designed primer pair G268F/G1096R or G699F/G1134R whereas, no amplification was noted for closely-related species from other genera.

研究结果显示，利用G268F/G1096R及G699F/G1134R两组Gordonia菌属特异性引子，可有效区别Gordonia菌属与其他放线菌菌属，并且利用此侦测技术已从台湾分离出36株Gordonia分离株。

Choose 37 ACGM primers to PCR amplification from 55 gramineae germ plasm, 569 bands are obtained, and all of the bands are polymorphism.

利用37对ACGM引物对55份禾本科种质进行PCR扩增，共扩增出569条谱带，皆为多态性条带。

Methods From Feb 2006 to Jun 2006,188 hospitalized children in Shenzhen children s hospital, were collected deep tracheal aspirate at the time of hospitalization. The respiratory tract secretions were immediately sent for bacterial culture with 3 kinds of medium:ordinary medium, Hemophilus influenzae selective medium, Streptococcus penumoniae selective medium. Then we extracted the total nucleic acids from secretions, and detected Mycoplasma pneumoniae by single fluorescent quantitation PCR. Simultaneously, 14 respiratory tract pathogenic bacterium and Mycoplasma pneumoniae were detected by Target Enriched multiplex PCR. Amplification products were identified by the Luminex100 suspension array.

确诊为社区获得性肺炎的患儿188例，在入院当天采集深部呼吸道吸引物，用普通培养基和肺炎链球菌、流感嗜血杆菌选择性培养基进行细菌培养，然后提取深部呼吸道吸引物中病原体的DNA，采用荧光定量单PCR的方法检测肺炎支原体，并对同一标本采用靶序列富集多重PCR技术同时扩增肺炎链球菌、流感嗜血杆菌、金黄色葡萄球菌、肺炎克雷伯菌、大肠杆菌、嗜肺军团菌、铜绿假单胞菌、鲍曼氏不动杆菌、脑膜炎奈瑟氏菌、阴沟肠杆菌、奇异变形杆菌、化脓链球菌、粪肠球菌及屎肠球菌14种呼吸道病原菌和肺炎支原体的靶基因，扩增产物用Luminex100多功能悬浮点阵仪检测。

Heterodon specimens was successfully extracted by the steps of removing the formaldehyde through improved gradual dehydration first and extracting the genomic DNA by the traditional phenol-chloroform method then. After primers designed, the PCR amplification was carried out, and a 205 bp of the control region of mtDNA from all of the ten specimens was successfully amplified.

本实验通过改进的酒精梯度浸泡法去除标本中的甲醛，然后用酚-氯仿抽提法成功地提取到了铜鱼标本的基因组DNA；设计引物后进行了线粒体和微卫星的PCR扩增，扩增产物经银染检测。

Heterodon specimens as successfully extracted by the steps of removing the formaldehyde through improved gradual dehydration first and extracting the genomic DNA by the traditional phenol-chloroform method then. After primers designed, the PCR amplification was carried out, and a 205bp of the control region of mtDNA from all of the ten specimens was successfully amplified.

本实验通过改进的酒精梯度浸泡法去除标本中的甲醛，然后用酚－氯仿抽提法成功地提取到了铜鱼标本的基因组DNA；设计引物后进行了线粒体和微卫星的PCR扩增，扩增产物经银染检测。

The psbL-psbJ region of chloroplast DNA of 8 and 2 Hordeum species or subspecies from China and other countries, respectively, were investigated by PCR amplification and direct sequencing.

对8份来源于中国和2份来自国外的不同大麦属物种或亚种叶绿体基因组psbL～psbJ区进行了序列测定。

In this study, we refer to the NCBI database to design primers which are used for amplification of the gene fragment of hepatopancreatic parvovirus、infectious hypodermal and hematopoietic necrosis virus and taura syndrome virus.

本研究从NCBI database资料中，分别在肝胰小病毒(hepatopancreatic parvovirus， HPV)、传染性皮下及造血组织坏死病毒(infectious hypodermal and hematopoietic necrosis virus， IHHNV)和桃拉病毒(taura syndrome virus， TSV)等三种常见之虾病毒中，各选出一段壳蛋白基因，利用重组蛋白的方式，在大肠杆菌中大量表现。

In this study,Amplified fragment length polymorphism analysis was carried out by using 23 pairs of EcoRI-NN/MseI-NNN selective primers in amplification of the DNAs from reciprocal F_1 hybrids of Cucumis hystrix Chakr.and C.

为了研究细胞质背景对基因组序列丢失的影响，利用23对Eco RI-NN/Mse I-NNN选择性引物对Cucumishystrix与栽培黄瓜C.sativus的正反交F_1、异源双二倍体及亲本DNA进行AFLP分析。

Therefore, we proposed a unidirectional amplification scheme which facilitates bidirectional transmission by using an interleaver.

因此我们提出了利用间隔器组成一个单向放大路径的结构用以达成双向传输的效果。

ERIC, a pair of rep-PCR primers, can amplify NSa-specific band. Using the amplification band patterns of two pairs of primers, which are REP and ERIC, six types of male sterile cytoplasm can be distinguished. 2. A cDNA library for CMS-associated genes was constructed by suppression subtractive hybridzation with the sterile line and its isonuclear maintainer.

利用特异引物扩增的结果表明NSa不育胞质与Pol、Ogu不育胞质不同，一对rep-PCR引物ERIC能扩增到NSa的特异带，而且REP和ERIC两对引物相结合能将NSa不育胞质与油菜原有的不育细胞质区分开，从分子水平上证明了NSa不育胞质的新颖性。

When I started coding, I had problems with the ASF file writer.

当我开始编码，我曾与ASF文件作家的问题。

I know that in one of the schools the boys have risen up in the classroom and enlisted in a mass.

如果你们也和他们一样，是集体自愿的话，你们的老师将是多么地光荣啊。