查询词典 amplification

OBJECTIVE: After purification and amplification in vitro, the bone marrow mesenchymal stem cells were induced into chondrocytes, and then implanted them at the bottom of nano-hydroxyapatie/chitosan scaffold for combined culture. The aim was to investigate the feasibility of them used in tissue-engineered chondral composite graft.

目的：将体外提纯、扩增的骨髓间充质干细胞诱导成软骨细胞，将其接种于穿&靴&的纳米羟基磷灰石/壳聚糖支架的底部上联合培养，探索其用于组织工程软骨复合体的可行性。

A cDNA for endo-β-1,4-glucanase was isolated by RT-PCR, and rapid amplification of cDNA ends reaction from taro leaves (Colocasia esculenta var. esculenta).

本研究利用RT-PCR及rapid amplification of cDNA ends的方法，自槟榔心芋叶片选殖内切型纤维素分解酵素cDNA。

To improve detection efficiency and result accuracy, we developed an improved multiplex PCR technique for specific detection of GMO.①The assay strategy involves multiplex PCR amplification of the target with several biotinylated primer pairs in one tube, followed by the hybridization of multiplex PCR products and membrane-bound oligonucleotide probes. The multiplex PCR/probe hybrid is detected colorimetrically using an alkaline phosphatase conjugate and a chromogenic substrate.

在多基因定性检测技术方面，本文在优化并确定了多基因PCR反应条件的基础上，采用了两种方法对多基因PCR产物进行鉴定：①采用共价结合方法将多个探针固定在Biodyne〓C膜上，将5'端标记生物素的多基因PCR产物与膜上的探针进行杂交及显色反应，首次建立了GMO的多基因PCR-膜相杂交检测方法，实现了一个反应体系中同时检测多种转基因成分的目标，提高了检测效率与准确率。

The GPA1 gene was obtained via PCR amplification and was cloned in the two-hybrid DNA binding domain vector pGBKT7 The combinant plasmid was designated as pGBKT7-Ga.β-galactosidase assay indicatedthat Got did not have the property of self-activation. After pGBKT7-Ga was transformed to yeast pJ69-4A, we transformed Arabidopsis vegetative tissue two-hybrid cDNA library plasmids to yeast pJ69-4A containing pGBKT7-Ga.

通过PCR扩增得到GPA1基因并将其克隆到双杂交DNA结合域载体pGBKT7中，得到的重组质粒命名为pGBKT7-Gα，β—半乳糖苷酶活性鉴定表明Gα不具有自激活特性，将其转化到酵母菌pJ69-4A中，再以此为受体菌转化拟南芥绿色营养组织cDNA文库质粒。

The research on the precision of synchronizing control system of hydraulic 4-roller crimper that combines common electromagnetic value with mechanical reacting amplification mechanism and the methods to solve the problems of cylinder synchronous running havebeen performed.

论述了W12—25×2000型液压四辊卷板机的同步控制系统改进过程中遇到的问题及研究解决方法，介绍了采用普通电磁换向阀配以机械反馈放大机构解决液压四辊卷板机同步运动的控制精度问题。

The procedure and system could meet the demands for genome SRAP amplification in Cucumis melo L.

结果表明，该程序和体系能够较好的满足甜瓜基因组SRAP扩增的要求。

In conclusion, the optimization of SRAP-PCR amplification system can be used to study genetic relationship,systematic evolution and genetic diversity of cynodon dactylon.

说明该优化的SRAP-PCR体系可用于野生狗牙根不同种间亲缘关系、系统进化和遗传多样性等领域的研究。

Full-length cDNA sequence of NPY from Barbodes sinensis Based on multiple alignment of a number of Cyprinidae NPY genes, two special primers of 3'cDNA ends were designed for amplification of the 3'cDNA ends of NPY genes and the sequence was acquired from the cDNA templet of the brain of S.sinensis.

中华倒刺鲃基因推导氨基酸序列的分析中华倒刺鲃NPY的cDNA编码区核苷酸长度为291bp，编码96个氨基酸，由信号肽(28个氨基酸)、NPY成熟肽(36个氨基酸)、三肽加工位点(3个氨基酸)和连接肽(29个氨基酸)组成。

It means that if, despite precautions, the Frankenbug does get out, its entirely harmless presence would be detectible in any given sample by straightforward DNA amplification technology of the sort used in genetic fingerprinting.

它意味着，虽然全然无害，但如果预防失灵，这个&弗兰肯斯坦&小虫逃了出去，用在基因指纹图谱上的DNA放大技术就可以直接将它在任何样本中检测出来。

The amplified fragment length polymorphism technique was used to assess the genomic DNA polymorphism of four species Emberiza (cioides, leucocephala, tristrami and spodocephala). Eleven pairs out of thirty-nine AFLP primer pairs produced reproducible polymorphic DNA amplification patterns.

利用扩增片断长度多态性技术分析了鹀属4种鹀（三道眉草鹀Emberiza cioides，白头鹀Emberiza leucocephala，白眉鹀Emberiza tristrami，灰头鹀Emberiza spodocephala）基因组DNA的多态性。

It's bell can reach over a meter in diameter.

它的身体最大可以达到直径1米多。

Now they are overdue and I shall have to pay a fine.

现在他们过期了，我不得不付罚款。