查询词典 amplification

RT-PCR amplification of ginseng β-amyrin synthase gene was successfully performed based on the total RNAs extracted from ginseng hairy roots induced by Agrobacterium rhizogenes A4 using a modified guanidine isothiocyanate-method.

以发根农杆菌A4菌株诱导的人参发根为材料，用改良的异硫氰酸胍法提取总RNA，得到了纯度好的完整的总RNA。

80 Individuals of ATA-A F2 plants were used for RAPD molecular mapping of the monoecious gene by bulk segregant analysis. 840 random primers were chosen for polymerase chain reaction amplification between monoecious pool and andromonoecious pool, the results showed that: primer S128 was tightly linked to the monoecious character, and primer S493 was tightly linked to the andromonoecious character.

以甜瓜单性花系ATA-A系与两性花品种YJX-1的杂交F2代的80个单株为材料，利用群分法进行单性花基因有关RAPD分子标记研究，筛选分析了840个10bp随机引物的PCR扩增谱带，结果表明，随机引物S128在1180bp，S493在930bp处产生与性别相关的特异性条带，S128的特异条带与单性花性状相关，S493的特异条带与两性花性状相关。

MATERIALS AND METHODS: Using telomeric repeat amplification protocol assay and ABC immunohistochemical technique, the level of telomerase activation and PCNA expression were evaluated in 29 skin lesions of arseniasis patients and 10 cases of normal skin.

材料与方法：采用端粒重复序列扩增法和免疫组化ABC法检测29例砷中毒患者病损皮肤及10例正常皮肤组织中的端粒酶活性和PCNA表达情况。

Five methods namely improved CTAB method, CTAB-silica extraction method, SDS method, low pH extraction method with high salt and Zhou shiliang method were adopted to extract genome DNA from the leaves of Citrus aurantium. And three methods including ultraviolet spectrophotometer, agarose gel electrophoresis and SSR-PCR amplification were used to detect extracted sample DNA.

以枳壳叶片为试验材料，分别采用改良CTAB法、CTAB-硅珠法、SDS法、高盐低pH值法和周世良法5种方法提取枳壳基因组DNA，并通过紫外分光光度计、琼脂糖凝胶电泳及SSR-PCR扩增3种方法对所提取的DNA样品进行检测。

Furthermore, cell culture and amplification are comparatively easy so we can get a great quantity of cells applied by autografting and allografting.

目的：探讨由脂肪组织分离获得脂肪基质细胞的方法及其在体外经诱导培养后向成骨细胞分化的潜能。

Some taxon-selective primers that were intended to be specific to fungi and basidiomycetes respectively have been designed and tested to characterize fungal symbionts by amplification of specific sequences in the nuclear ribosomal unit.

大量DNA分析新技术在丛枝菌根真菌的分子遗传、分类鉴定、种间及种内亲缘关系、菌株持久性等方面得到应用，与传统菌根研究方法相比，表现出巨大的优越性。

Transgenic plants were obtained by basta screening and PCR amplification.

转化T1代植株经除草剂Basta筛选和PCR扩增获得转化植株。

Significant differences in cytosine methylation level among the three tissues and the same changing tendency in two inbred lines were detected. Both MSAP (methylation sensitive amplification polymorphism) ratio and full methylation level were the highest in bracteal leaf, and the lowest in tassel.

结果表明，3个组织甲基化水平存在一定差异，并在两自交系中表现出相同的变化趋势，即雌穗苞叶的甲基化敏感扩增多态性比率和全甲基化率最高，穗位叶次之，雄穗最低。

In the first case, the products of the MFW4/31 amplification were loaded when bromophenol blue dye from MFW7/9 sample migrated to a 3-cm distance from the wells, while in the second case, MFW17/26 products were loaded when the dye from MFW13/20 reached 4.5 cm.

在第一种情况下，当溴酚蓝染料从MFW7/9 样品迁移到离加样穴 3-cm 距离时加入MFW4/31 扩增产物，而在第二种情况下，当溴酚蓝染料从MFW13/20 样品迁移到离加样穴4.5 cm距离时加入MFW17/26 产物。

In this research, we extracted the genomic DNA from the Buxus sinica var. parvifolia leaves by using the modified CTAB approach and then used as the template DNA. Base on the invariable quantity of TaqDNA, we att empted to establish and optimize ITS-PCR amplification system for Buxus sinica var. parvifolia by employing L9 (3^4) orthogonal design with four factors, i.e. template DNA, Mg(superscript 2+), dNTPs and primer.

本研究利用改良CTAB法从珍珠黄杨叶片中提取基因组DNA作为模板，在TaqDNA聚合酶量不变的基础上，利用正交设计L9(3^4)对4个因素（模板DNA、Mg(上标2+)、dNTP和引物）在3个水平上对珍珠黄杨ITS-PCR反应体系进行优化。

It's not quite as simple as that.

并没有那么容易。

It debuted in 1992 in Japan and the United Kingdom, and 1993 in the United States.

它首次亮相于1992年在日本和英国，在美国1993年。