constructed

constructed的基本解释

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[变形] construct的过去分词

vt.

构造, 建造, 作图, 对...进行构思

n.

构成物

与constructed相关的网络例句 [注：此内容来源于网络，仅供参考]

Especial, forv=36 and 76, the methods to construct D_(3×4)K_(36 and D_(3×4)K_(76 are differentfrom the methods used above. The above small designs are constructed byusing complete orbit and abelian group while the D_(3×4)K_(36 is constructed byusing short orbit and the D_(3×4)K_(76 is constructed by using short orbit andnonabelian group.

特别对于υ=36和υ=76这两个值，我们构造时用到的方法与我们前面构造小设计时用到的方法不同，前面我们构造小的设计时，都是基于完整的轨道和Abelian群的，但对于这两个值我们是用短轨道和非Abelian群进行构造。

Methods: The human KDR promoter (-225 bp~+127 bp) was cloned by PCR. Subsequently, a recombinant adenoviral plasmid carrying KDR promoter for the HSV-tk gene was constructed with the AdEasy system. As a control, the plasmid pAdCMV-tk carrying CMV promoter for the HSV-tk gene was also constructed. 293 packaging cells were transfected by both newly-constructed plasmids and the infectious viruses AdKDR-tk and AdCMV-tk were generated. Then the KDR-producing cells and the KDR nonproducing cells (HepG2) were infected with AdKDR-tkand AdCMV-tk, followed by ganciclovir administration.

应用PCR克隆出人KDR基因启动子序列－225bp~+127bp，以AdEasy system为载体，构建携带受KDR启动子或CMV启动子调控tk基因表达的2种重组腺病毒质粒pAdKDR-tk和pAdCMV-tk，在293细胞中包装、扩增后，体外感染表达KDR的脐静脉血管内皮细胞系HUVEC和不表达KDR的肝癌细胞系HepG2，并给予不同浓度的丙氧鸟苷处理，5d后收集存活细胞并计数。

Abortus, amplify BP26 gene by PCR and clone to pMD18-T simple vector. Identify the constructed recombinant plasmid pMDBP26 by sequencing, then subclone to vector pET-28a. Transform the constructed recombinant plasmid pETBP26 to E. coli BL21(DE3) for expression under induction of IPTG. Purify the expressed product by histidine-binding resin column chromatography and identify by Western blot.

提取布鲁氏菌基因组DNA，用PCR扩增出BP26基因，并克隆到pMD18-T载体上，测序正确后，再亚克隆至pET-28a载体上，转化到大肠杆菌BL21(DE3)中，用IPTG诱导表达，经组氨酸结合树脂柱纯化，并对纯化后的表达产物进行Western blot鉴定。

与constructed相关的网络解释 [注：此内容来源于网络，仅供参考]

CONSTRUCTED SPECIFICATION：结构细节

COMBED 精梳 | CONSTRUCTED SPECIFICATION 结构细节 | CONTINUOUS PLACKET R折

CONSTRUCTED SPECIFICATION：精梳

色差 COMBED | 精梳 CONSTRUCTED SPECIFICATION | 结构细节 CONTINUOUS PLACKET R

CONSTRUCTED SPECIFICATION：框架细节

COMBED 精梳 | CONSTRUCTED SPECIFICATION 框架细节 | CONTINUOUS PLACKET R折

CONSTRUCTED SPECIFICATION：构造细节

COMBED 精梳 | CONSTRUCTED SPECIFICATION 构造细节 | CONTINUOUS PLACKET R折

constructed wetlands：人工湿地

人工湿地(Constructed Wetlands)是20世纪70年代发展起来的一种废水处理新技术,与传统的污水二级生化处理工艺相比.具有净化效果好、去除N与P能力强、工艺设备简单、运转维护管理方便、能耗低、对负荷变化适应性强、T程建设和运行费用低、出水具有一定的生物安全性、生态环境效益显著、可实现废水资源化等特点.