黑穗病

Ustilago crameri koern in our province is one of the major diseases in recent years, there are signs of increase.

谷子黑穗病是我省谷子的一个主要病害，近年来有加重的迹象。

Sugarcane smut caused by Ustilago scitaminea is a worldwide disease and causes serious damage to sugarcane.

甘蔗黑穗病是由黑粉菌（Ustilago scitaminea Syd。

Sugarcane,which is the most important industrial material crops in China, is affected by pathogens because of abscence the disease resistant sugarcane cultivals.The most important diseases are caused by fungi,eg Ustilago Scitaminea Syd.

甘蔗是我国最为重要工业原料作物，近年来由于高产、高糖甘蔗品种的大面积使用，又缺乏相应的抗病品种，甘蔗病害日益严重，尤其是甘蔗的黑穗病、梢腐病等真菌病害常在蔗区大面积流行，严重影响甘蔗的产量和质量。

The universal primers of ITS1 and ITS4 for fungi were used in amplification of genomic rDNA sequences. A target fragment was obtained by ITS-PCR. Then, the fragment is cloned into the vector of pMD18-T before sequencing. The result of sequencing showed that the fragment is 692 bp in length. The results of Blast in Genbank and the analysis of fungal rDNA systemic development show that the relation between this fragment to the fungi of Sporisorium is closest, while the relation between it to the fungi of Ustilago is much far. It suggests that the name of Ustilago scitaminea for sugarcane smut isn't consistent to the result of rDNA systemic development analysis. It still needs more experiments to support this hypothesis.

用真菌rDNA序列分析的通用引物ITS1和ITS4，通过ITS-PCR得到目的片段，克隆在pMD18-T载体上后进行测序，结果显示该片段的长度为692 bp，与Genebank中已报道的真菌序列进行Blast和系统发育树分析，表明所获得的序列与Sporisorium属真菌具有更高的亲缘关系，而与Ustilago属真菌的亲缘关系较远，这与一直沿用的甘蔗黑穗病菌的命名似乎并不吻合，有待进行更多的研究以证实。

The culture filtrate of strain LN-176 was tested for light absorption at 260nm and 280nm, and the results showed that the protein content in the culture filtrate significantly increased. After ammonium sulphate precipitation, the supernatant and the precipitation were separated to testify their antagonistic activity, and it was found that the precipitation was inhibitory to Ustilago hordeum , but the supernatant not.

LN-176菌株的发酵液在260nm和280nm下进行吸光度测定，结果表明发酵液中蛋白质含量明显升高；发酵液经硫酸铵盐析后，取上清液和沉淀物分别做抗菌试验，发现沉淀物对青稞散黑穗病菌有拮抗活性而上清液无活性。

Sugarcane smut caused by Ustilago scitaminea Svd.

由甘蔗黑穗病菌（Ustilago sictaminea Syd。

Parasitic fungi causing smuts; sometimes placed in class Tiliomycetes.

引起黑穗病的寄生菌；有时被归于x中。

The group includes puffballs, shelf fungi, rusts, smuts, and mushrooms.

这个群包括马勃菌、架状菌、锈菌、黑穗病菌和伞菌

The study aimed to research the molecular marker of head smut gene of sweet sorghum by RAPD technology.

［目的］应用RAPD技术对甜高粱丝黑穗病基因进行分子标记研究。

Objective］ The study aimed to research the molecular marker of head smut gene of sweet sorghum by RAPD technology.

［目的］应用RAPD技术对甜高粱丝黑穗病基因进行分子标记研究。

I'm strongly against the death penalty — it's an eye for an eye.

我不赞成死刑——这是以牙还牙的报复行为。

And to get you the support you need, we're enlisting all elements of our national power: our diplomacy and development, our economic might and our moral suasion, so that you and the rest of our military do not bear the burden of our security alone.

并给你们所须的支援，我们正徵召国家所有各种的力量：我们的外交及发展，我们的经济力量与道德劝说，所以你们与其他军人不须要孤独地负起国家安全的责任。