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Methods: A model to evaluate lymphocyte proliferation stimulated with a polyclonal activator, concanavalin A, was established by vital dye carboxyl fluorescin diacetate succinmidyl ester labeling technique. Effects of the different doses of anisomycin on the lymphocyte proliferation were estimated by flow cytometry and MTT methods. The propidium iodide labeling technique was applied to assay the effect of the different doses of anisomycin on changes of the lymphocyte cell-cycle stimulated by ConA or by phorbol ester plus Ionomycin. The percentage of the expression level of CD69 and CD25 on the activated lymphocytes was evaluated by fluorescin-conjugated monoclonal antibody double labeling technique.

以活体染料羧基荧光素乙酰乙酸琥珀酰亚胺酯染色,建立在多克隆刺激剂刀豆蛋白A刺激下评价小鼠淋巴细胞增殖的模型,通过流式细胞术和MTT法分析茴香素在不同剂量下对淋巴细胞增殖的作用;采用碘化丙锭染色分析茴香素对ConA或佛波醇酯加离子素刺激的小鼠淋巴细胞周期变化的作用;利用荧光标记的单克隆抗体双染技术和流式细胞术观察茴香素对小鼠CD3~+T细胞早期及中期活化标志分子CD69和CD25表达的影响。

The batch seeded isothermal desupersaturation experiments of SPM in aqueous solution were carried out and the overall crystal growth kinetics parameters were determined by the nonlinear optimization techniques. Assuming that the volume diffusion rate at growth is equal to the mass transfer rate at dissolution under the same conditions, the two-step crystal growth rate parameters can be determined. At the fow supersaturation, the surface integration rate is proportional to relative surface supersaturation.

通过测量螺旋素均相成核动力学,获得了表面能的值,估算出表面熵因子的值在1.41~1.56之间,从而可以推断出螺旋素晶体生长的表面反应速率同表面相对过饱和度成线性关系,这种生长机理同第四章得到的结果是相吻合的;利用估算的表面熵因子的值,把螺旋素晶体看作为简单的立方科瑟尔晶体,根据能量最低原理,对螺旋素的晶体生长进行了模拟。

In the four ecosystem types, species of Alternaria and Ulocladium had wider niche breadth and were of widely adaptive species, while species of Bipolaris, Cirrenaria, Curvularia, Doratomyces, Graphium, Myrothecium, Scolecobasidium, Scopulariopsis, Scytalidium and Stachybotrys had narrower niche breadth and were of narrowly adaptive species.

在上述四类生境中,链格孢属Alternaria和细基格孢属Ulocladium真菌具有较宽的生态位,为广适性物种;而平脐蠕孢属Bipolaris、卷旋孢属Cirrenaria、弯孢属Curvularia、矛束属Doratomyces、粘束孢属Graphium、漆斑属Myrothecium、齿梗孢属Scolecobasidium、帚属Scopulariopsis、节隔孢属Scytalidium和葡萄穂属Stachybotrys10个属真菌的生态位较窄,为狭适性物种。

In the group of inhaled allergens, the positive rate in order are mite mixture (dermadermatophagoides pteronyssinus, dermatophagoides farinae), mould mixture, weed mixture, tree mixture, cockroach, cat-dog epithelia, humulus lupulus; In group of food allergens, the positive rate in order are fish-shrimp-crab, egg white/yolk, milk, beef-mutton, cashew nut-peanut-soybean, mango and wheat.

吸入组过敏原阳性率依次为户尘螨粉尘螨、点青分枝孢交链孢、矮豚草蒿、柏榆梧桐柳三角叶杨、蟑螂、猫毛皮屑狗毛皮屑、葎草;食物组阳性率依次为鱼虾蟹、鸡蛋白鸡蛋黄、牛奶、牛肉羊肉、腰果花生黄豆、芒果、小麦。

The feedstuff was produced by using waste spent grains as the main raw materials and candida mycoderma, geotrichum candidum link and rh.

以大曲丢糟为主要原料,以假丝酵母、白地、根为生产菌,采用正交试验,得到最佳工艺条件为:丢糟∶麸皮=5∶1,假丝酵母∶白地∶根=1∶1∶2,在32℃下培养5d,得到的饲料营养丰富,易被动物吸收,是一种新型的饲料蛋白来源。

Result The phytopathogenic fungus Pythium aphanidermatum was isolated; 7 strains of antagonistic actinomycete, which had obvious inhibitory effects on Pythium aphanidermatum were screened. The inhibitory ring of No.8 strain was 13.3 mm, and reached 88.3% when cultivated for 120 h.

伽师甜瓜猝倒病的病原菌为瓜果腐;筛选出7株对瓜果腐具有显著拮抗性的生防放线菌,其中8号放线菌对瓜果腐的拮抗环宽度为13.3mm,其无菌发酵滤液对瓜果腐的抑菌率在培养120h时高达88.3%。

In bioconv ertion experiment for spiramycin of No.5 transformant, the bioconversion product was analysed with TLC, bioautography, HPLC and mass spectrum.Results showed that the bioconvert product is propionyspiramycin. Propionyl acylase gene was l ocolized on 0.53 kb insert DNA fragment of recombinant pasmid pLJM95. Analysis result of DNA sequence showed that content of G+C is 68.2% for 0.53kb insert DNA fragment .

以No.5转化子对螺旋素进行生物转实验,其转化产物经薄层层析,生物显迹,高压液相色谱及质谱分析表明,与标准丙酰螺旋素一致,这说明 No.5 转化子具有将螺旋素生物转化为丙酰螺旋素的能力,丙酰化酶基因位于重组质粒,pIJM95 0.53kb 插入DNA片段上。

Intravenous medium and high erythromycin and azithromycin dose had obvious exciting effect on spontaneous afferent nerve discharge (erythromycin: 8.34 ± 0.37, 8.54 ± 0.26 vs 7.78 ± 0.23, 7.84 ± 8.27; azithromycin: 8.57 ± 0.43, 8.28 ± 0.38 vs 7.74 ± 0.21, 7.86 ± 0.30) and gastric distention-induced afferent nerve discharge of subdiaphragmatic vagus nerve (erythromycin: 8.54 ± 0.34, 8.61 ± 0.20 vs 8.13 ± 0.36, 8.19 ± 0.21; azithromycin: 8.54 ± 0.30, 8.42 ± 0.21 vs 8.24 ± 0.22, 8.22 ± 0.19), which was statistically significant compared with the control and low dose groups.

结果: 静脉注射小剂量红素和阿齐素自发放电和对扩胃诱发放电均无显著变化, 20 min后中、大剂量红素和阿齐素注药后自发放电(红素: 8.34±0.37, 8.54±0.26 vs 7.78±0.23, 7.84±8.27;阿齐素: 8.57±0.43, 8.28±0.38 vs 7.74±0.21, 7.86±0.30)和对扩胃诱发放电均增强(红素: 8.54±0.34, 8.61±0.20 vs 8.13±0.36, 8.19±0.21;阿齐素: 8.54±0.30, 8.42±0.21 vs 8.24±0.22, 8.22±0.19)与相应的对照组和小剂量组间差异有统计学意义。

It was susceptible to doxycycline, azithromycin, josamycin, especially the susceptibly of doxycycline reaches to 98.8%. Doxycycline can be considered as the first choice of antibiotic to treat biotype one of Ureaplasma urealyticum in the near future.

女性生殖道中Uu生物一群主要对喹诺酮类药物耐药,敏感药物主要为强力素,阿歧素及交沙素,尤其强力素的敏感性达到了98.8%,近期内可作为治疗Uu生物一群的一线用药。

This study demonstrated the native-like conformation and interactions between molecules on a proper physically charactering resin, which additionally provides evidence of on-bead screening on the basis of molecular recognition.

结果表明:固载在PEGA树脂上万古素在DMSO中与万古素自身的构象相同,而且固载在PEGA树脂上万古素和Ac〓-L-Lys-D-Ala-D-Ala的相互作用方式与万古素自身和配体形成复合物的方式相同。

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If you are unfortunate enough to the lovelorn, please tell me, I will help you out, really, please contact me!

如果你不幸失恋了,请告诉我,我会帮助你摆脱困境,真的,请联系我啦!

China's plan to cut energy intensity by 20 percent and pollutant discharges by 10 percent between 2006 and 2010 is a case in point.

中国计划在2006年到2010间降低20%的能源强度和减少10%的主要污染物排放,就是一个这样的例子。

Well, Jerry would rattle off all the details of that movie.

那么,杰瑞会急促背诵那部电影所有细节。