霉

After the supplement of L-Leu, extracellular isovaleric acid was accumulated in vitro and then reabsorbed for eutilization, the ultimate relative content of isovalerylspiramycin II, isovalerylspiramycin III was increased by 41% and 50% respectively, and the relative content of total isovalerylspiramycins increased by 41.9% with the biological titer of biotechspiramycin being equal to that of the control group.

添加Leu对初级代谢影响不大，异戊酸在胞外积累，随后被重新吸收利用，最终异戊酰基螺旋霉素II和异戊酰基螺旋霉素III组分分别提高了41%和50%。同时总异戊酰基螺旋霉素相对含量也比对照提高了41.9%，效价和对照基本一致。

When reached logarithm growth phase, it was poured or the medium was removed. 5 mL medium containing 10 mg/L mitocin-C was added , kept at 37 ℃, and then cultured in saturated humidity warm box with the CO2 of 0.05 fraction volume for 2 or 3 hours. It was coated with 0.1% gelatin in 6-hole plate, laid for over 30 minutes, and then threw away or the medium with mitocin-C was removed, washed with phosptat buffer for 5 times so as to remove the mitocin-C. 2 mL 0.05% trypsin digestive cells were added, and it was observed under microscope. When crevice appeared, cells became round (about 2－4 minutes), digestion was stopped by adding medium of the same volume, and blew up with straws repetitively to make it into monoplast suspension. Special-used cover glass was put in the center of cell counting chamber. Cells were sucked in by glass siphon, and cell suspension flew out at bucket of up or down-sides of counting chamber, until the cover glass was filled with fluid. Living cell were inoculated at concentrations of 3×108, 5×108, 1×109 L－1 after their number counted.

选取2～5代的小鼠胚胎成纤维细胞，待其至对数生长期倒掉或吸掉培养基，加入含10 mg/L丝裂霉素C的细胞全培养液5 mL，置37 ℃，体积分数0.05的CO2饱和湿度温箱中培养二三小时，在6孔板中加入0.1%明胶包被，放置30 min以上，倒掉或吸掉含丝裂霉素C的培养基，磷酸盐缓冲液清洗5遍，尽量除去丝裂霉素C，加入2 mL 0.05%的胰蛋白酶消化细胞，镜下观察，当细胞间出现裂隙，细胞变圆时（约2～4 min），立即加入等量的全培养液终止消化，并用吸管反复吹打，使之成为单细胞悬液，在细胞计数板中央放置专用的盖玻片，用玻璃虹吸管吸取细胞，让虹吸管在盖玻片上或下侧的计数板凹槽处流出悬液，至盖玻片下被液体充满为止。

The first method adopts different kind microbes to ferment buckwheatextraction. The method can increase DCI and its derivation content in buckwheat seed solution, while itreduces sugar content in extraction. The research adopts Saccharomyces cerevisia, Mucor, Bacterium coli, Torulopsis apicola, Aspergillus oryzae Bacillus subtilis, Candida mycoderma, Black mold that inoculate inbuckwheat extraction. Determine content change of various compotent with tracing.

方法一采用不同种类微生物发酵荞麦提取物，增加荞麦籽粒水溶液中D-手性肌醇及其衍生物的含量，同时减少提取物中总糖含量，本研究采用酿酒酵母、毛霉、大肠杆菌、蜜蜂生球拟酵母、米曲霉、枯草芽孢杆菌、解酯假丝酵母、黑曲霉接种于荞麦提取物中进行培养，并跟踪测定提取物中各种成分的含量变化。

Since OPP and its sodium salt are nontoxic, odorless, of high phytocidal activity, good disinfecting and mould-inhibiting properties, they can be used as antiseptic, used for fruits and vegetables especially mandarins to inhibit mould, and used to treat lemons, pineapples, melons, apples, pears, peaches, tomatoes, etc, to protect them from putrefying to the greatest extent.

由于邻苯基苯酚及其钠盐除莠活性很高，并且有广谱的杀菌除霉能力，而且无毒无味，是较好的防腐剂，可用于水果蔬菜的防霉保鲜，特别是用于柑桔类的防霉，也可以用于处理柠檬、菠萝、瓜、果、梨、桃、西红柿等，可使腐烂降到最低限度。2。

Saprolegnia disease :the most common pathogens are two generas of Saprolegnia and Achlya ,and not strictly selectivity on the host.

水霉病：病原最常见的是水霉和绵霉两属，对寄主没有严格的选择性。

Validamycins have been one of the most popular and economical biopesticides in China, by microbial transformation of which validoxylamine A and valienamine can be produced. Validoxylamine A has strong inhibition on trehalase and it is a new promising insecticide. Valienamine demonstrates strong glucosidase inhibitory activity, which can also be used as intermediate in the synthesis of medicines for diabetes.

井冈霉素目前已成为我国最经济最受欢迎的生物农药之一，用微生物转化法分解井冈霉素可以得到井冈霉亚基胺A和井冈胺，前者对海藻糖酶有强烈的抑制作用，有望开发成为新型生物杀虫剂，而后者是一种糖苷酶抑制剂，也可作为合成降糖药的原料。

The uropoiesis genital tract mycoplasma infects the Uu masculine gender rate to be highest, the feminine masculine gender rate is highest; The mycoplasma medicine sensitive first choice medicine is the force mildew element, junction Sha Meisu, the content mildew element; Must use the mycoplasma appraisal agaragar culture medium to make the mycoplasma proof raise.

泌尿生殖道支原体感染Uu阳性率最高，女性阳性率最高；支原体药敏首选药物是强力霉素、交沙霉素、美满霉素；必须使用支原体鉴定琼脂培养基做支原体确证培养。

The bioassay indicated that some of them possess certain fungicidal activity against Botrytis cinerea, Gibberlla zeae, Rhizoctonia solani, Venturia nashicola at a dose of 50 μg/mL. The EC50 value of title compounds B-8、B-9、B-10、B-12 was reached 1.0, 0.2, 1.2 and 0.4 μg/mL against B. cinerea, the effect was approximated or better than pyrimethail and EC50 value of title compounds B-8, B-10, B-11 was reached 0.07, 0.2 and 0.05 μg/mL against G. zeae, the effect was better than carbendazim.

在50μg/mL浓度时，该系列化合物对番茄灰霉病菌Botrytis cinerea、小麦赤霉病菌Gibberlla zeae、水稻纹枯病菌Rhizoctoniasolani、梨黑星病菌Venturia nashicola菌丝生长均具有很好的抑制活性，其中化合物B-8、B-9、B-10、B-12对番茄灰霉病菌的EC50值分别为1.0、0.2、1.2、0.4μg/mL，接近或优于对照药剂嘧霉胺，B-8、B-10、B-11对小麦赤霉病菌的EC50值分别为0.07、0.2、0.05μg/mL，优于对照药剂多菌灵。

An antibiotic that is obtained from cultures of the bacterium Streptomyces verticillus and used in the form of its sulfate for the treatment of various neoplasms.

博来霉素，争光霉素从细菌培养中获得的一种抗生素轮丝链霉，用其硫酸盐形式治疗各种瘤

The relative cross-reactivity of PA with AFB-1,ZON,T-2,FB were less than 0.01%and with an affinity constant for PA of about 1.54×10~8 liters per mol.It offered a foundation for developing immunoassay method for PA.

单克隆抗体与黄曲霉毒素B1、玉米赤霉烯酮、T-2毒素和伏马毒素B1均无交叉反应率，单抗的亲和常数为1.54x10~8 L/mol，为进一步建立青霉酸的免疫学快速检测方法提供了基础。3。

What are your goals and strategies for growth?

你的成长目标和策略是什么？

And unto the angel of the church in Sardis write; These things saith he that hath the seven irits of God, and the seven star I know thy works, that thou hast a name that thou livest, and art dead.

3：1 你要写信给撒狄教会的使者，说，那有神的七灵和七星的，说，我知道你的行为，按名你是活的，其实是死的。