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Results The results showed that the average concentration of the bacteria, fungi and total microbes in the outdoor environment were 5.04×10^4, 3.75×10^3 and 5.41×10^4 cfu/m^3 respectively and the fungi accounted for 6.92% of the total. The preliminary identification showed that the dominant bacteria mainly were Micrococcus, Bacillus, Pseudomonas and Staphylococcus and the average percentage were 37.3%, 21.0%, 10.3% and 9.5% respectively. Of the five fungi the dominant ones were Aspergillum, Penicillium, Mucor and Rhizopu. The average percentage were 42.0%, 36.0%, 4.4% and 4.2% respectively. Conclusion The outdoor air of Hefei has been polluted by airborne microbes.

结果 合肥城区空气细菌总数均值为5.04×10^4cfu/立方公尺,真菌总数均值为3.75×10^3cfu/立方公尺,合计为5.41×10^4 cfu/立方公尺,其中真菌占6.92%;对不同采样点的空气细菌和真菌进行了初步鉴定,优势细菌属为微球菌属、芽孢杆菌属、假单胞菌属和葡萄球菌属,分别占总细菌的37.3%,21.0%,10.3%,9.5%;真菌共检出5属,其中优势菌属为曲属、青属、毛属、根属,分别占总真菌的42.0%,36.0 %,4.4%,4.2%。

Totally,four AMF species(Glomus mosseae,G.hoi,G.sp.1 and G.sp.2)were identifiedfrom 49 farmland soil samples.G.sp.1 and G.hoi mostly distributed in Grey Luvisol;G.mosseae mostly in Dark-Brown Chernozem;G.sp.2 mostly in Dark-Grey Chernozem.

从49个农田土壤样品中鉴定出何氏球囊、摩西球囊、Glomus sp.1和G.sp.24种VA菌根真菌。G.sp.1和何氏球囊主要分布于灰色淋溶土中;摩西球囊主要分布于暗棕钙土中;G.sp.2主要分布于灰漠钙土中。

The mechanism of GCT restrained by ADM: To determine the mechanism of GCT restrained by ADM, the GCT cells were cul- tured with ADM (the dense of ADM after adding were 0.04μg/ml, 0.4μg/ml and 4μg/ml) for 72 hours. The GCT cell were observed by microscope and electron lens, analyzed by flow cell counter and DNA electrophoresis.

素抑制骨巨细胞瘤的机理:为探讨阿素抑制骨巨细胞瘤的机理,我们用阿素与骨巨细胞瘤细胞悬液共同培养72小时(阿素最终浓度分别为0.04μg/ml,0.4μg/ml及4μg/ml),搜集细胞,用光镜、电镜观察及用流式细胞仪、DNA电泳进行分析。

The results indicated that the strain was Mracemosus fresenius which belonged to Racemousus, Muco, Mucoraceae, Mucorales, Phycomycetes in Fungi. Its growth temperature was under 37℃, and the optimal temperature was determined to 28℃.

结果表明,该菌株为真菌门、藻菌纲、毛目、毛科、毛属、总状枝毛组中的总状枝毛

Happy to doxycycline, tetracycline 36.63%, the highest sensitivity, all above 95%, secondly, to clarithromycin 90.98%, 82.71% azithromycin and roxithromycin 21.8%, to 24.82% ciprofloxacin 41.35% levofloxacin, etc, and 44.36% poor sensitivity conclusion: mycoplasm of common antibiotics have different levels of resistance to the early clinical, so for suspected cases for inspection in order to understand the drug susceptibility, thus, the reasonable use.

对强力素,美满素,四环素,交沙素的敏感性最高,都高达95%以上,第二为克拉素90.98%,阿奇素82.71%,而对罗红素21.8%,环丙沙星24.82%,氧氟沙星41.35%,司巴沙星44.36%等敏感性差结论:支原体对常用抗生素有不同程度的耐药性,因此临床上对尽早的对疑似病例进行送检以了解药药敏情况,从而合理,规范的用药。

Happy to doxycycline, tetracycline 36.63%, the highest sensitivity, all above 95%, secondly, to clarithromycin 90.98%, 82.71% azithromycin and roxithromycin 21.8%, to 24.82% ciprofloxacin 41.35% levofloxacin, etc, and 44.36% poor sensitivity conclusion: mycoplasm of common antibiotics have different levels of resistance to the early clinical, so for suspected cases for inspection in order to understand the drug susceptibility, thus, the reasonable use.

对强力素,美满素,四环素,交沙素的敏感性最高,都高达95%以上,其次为克拉素90.98%,阿奇素82.71%,而对罗红素21.8%,环丙沙星24.82%,氧氟沙星41.35%,司巴沙星44.36%等敏感性差结论:支原体对常用抗生素有不同程度的耐药性,因此临床上对尽早的对疑似病例进行送检以了解药药敏情况,从而合理,规范的用药。

The results showed that they are Stemphylium sp, Fusella Sacc, Alternaria sp, Fu5ari urn sp, Gibberella sp, Sphacelia sp, Actinomyces, Dematium Pers.

初步鉴定结果分别属于匐柄、丛梗孢属、交链孢属、镰孢属、赤属、蜜孢、放线菌属和暗梗单孢属。

The progress in research of the biology effect of γ—Linolenic acid 、and its production by mortierella,thamnidium elegans,cunningnamella elegans,mucor mucedo'fermentation were summarized in this article.

本文主要综述了γ—亚麻酸的生物学作用,以及被孢,雅致枝,雅致小克银汉,根和高大毛发酵生产γ—亚麻酸的研究进展。

Methods Indirect immnofluorescence assay was applied to identify specificity of the two groups of monoclonal antibodies prepared with crude antigen and recombinant antigen of Aspergillus fumigatus,respectively.Two different double monoclonal antibody sandwich ELISA assays established with the two groups of antibodies were performed to detect antigents in the cell culture supermatants of 19 common species of Aspergillus,Penicillium marneffei,and 5 species of Candidas.

采用天然烟曲抗原免疫,获得广谱针对曲抗原的单克隆抗体;采用重组烟曲抗原获得特异性针对烟曲抗原的单克隆抗体,用间接免疫荧光鉴定,并分别建立2种双抗体夹心ELISA法,对19种常见的环境和临床分离曲株、马尔尼菲氏青菌及念珠菌培养液进行检测。

LC3-FITC had been used to monitor autophagy by immunofluorescence. It showed that there are a high level of LC3 puncta in HCQ+DOX or BAF +DOX treated cells, and in rapamycin treated cells, moderate level in HCQ, BAF, or DOX singly treated cells, and low level in 3-MA+DOX, and there was diffuse fluorescence in untreated or 3-MA treated cells.

内源性LC3免疫荧光染色显示,羟氯喹+阿素、BAF+阿素及雷帕素处理组MM细胞胞浆可见大量颗粒状荧光,羟氯喹、BAF、阿素单用组仅有中等量颗粒状荧光,而3-MA及3-MA+阿素组胞浆荧光信号基本呈弥散分布,类似于未处理对照组。5。

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