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In order to diagnose disease caused by muscovy duck parvovirus rapidly and accurately, and at same time, further study the taxonomic relationship between muscovy duck parivovirus and goose parvovirus, two pairs of PCR primers (LHMP1/LHMP2 and LHGP1/LHGP2) were designed respectively base on complete nucleotide sequences of Muscovy duck and Goose parvoviruses registered in the GenBank, The LHMP1 for the upper one and the LHMP2 for the lower one, corresponding to the specific nucleotides sequence 454-472 and 1173-1155 respectively in MDPV, and the LHGP1 for the the upper one and the LHGP2 for the lower one, corresponding to the specific nucleotides sequence 454-472 and 1173-1155 respectively in GPV.

为了能快速、准确地对番鸭细小病毒病进行诊断,同时为了能进一步弄清番鸭细小病毒与鹅细小病毒在分类学上是一种怎样的关系,比较GenBank中MDPV和GPV的基因序列,针对这两种病毒左阅读框编码非结构蛋白基因序列的相同区段,分别构建两对PCR引物。第一对引物LHMP1/LHMP2是根据MDPV的基因序列设计的,上游引物针对MDPV基因序列的454~472;下游引物针对基因序列的1173~1155,为19个碱基,扩增跨幅为719bp。

The result shows that the requirements and testing methods for flammability of building materials are similar between the United State and Canada, and they mainly are engaged in the test of combustibility and flame spread. In European Union, a new classification system for flammability of building materials was implemented in 2001, which includes two systems; one is for floor coverings and the other for all other building units. In Japan, the interior materials are classified into non-combustible, quasi noncombustible and fire-retardant materials according to Cone Calorimeter method (ISO 5660). China has established a non-combustible test, a three-class classification system for combustible wall and ceiling interior finishing materials and a two-class classification system for combustible floor coverings.

通过研究认为:美国和加拿大对建筑材料燃烧性能的要求和试验方法基本相似,他们主要针对材料的燃烧性、火焰传播进行检测;欧盟于2001年颁布了新的燃烧性分级体系,该分级体系包括专门针对铺地材料部分和针对其他所有建筑构件材料和产品部门;日本采用锥形量热计法(ISO 5660)将内装饰材料分为不燃、准不燃和阻燃材料3个等级;我国对建筑材料的试验方法包括不燃性试验方法、墙和天花板内装饰材料的三级分级体系和铺地材料两级分级体系。

First mostly contraposing hull deck structure it goes along FEA analysis, discuss the different influence degree of calculational results between the different simplifications of the mathematical model, thereinto mostly analyse five different simplifications of the deck model; Second contraposing hold section and hull deck structure it goes along FEA analysis together, discuss the different influence degree of calculational results between the different elements style, gridding number and gridding density, thereinto mostly analyse the difference between adopting 8 node shell element and 4 node shell element, nondense gridding and thick gridding, and numerous gridding number and few gridding number, In the end mostly contraposing hold section structure it goes along FEA analysis, discuss the different influence degree of calculational results between the different boundary conditions, thereinto mostly analyse the difference between adopting CCS boundary conditions and LR boundary conditions.

首先主要针对船体甲板结构进行分析研究,讨论结构简化不同对有限元计算结果的影响程度,其中主要研究分析了五种不同甲板结构简化模型;紧接着针对舱段与甲板结构一起进行分析研究,讨论单元类型,网格数量与密度的不同对有限元计算结果的影响程度,其中主要研究分析了分别采用8节点板壳单元与4节点板壳单元的不同,以及分别采用疏的网格与密的网格,多的网格数量与少的网格数量的计算结果的差异。最后主要针对舱段模型进行分析研究,讨论不同的边界条件对有限元计算结果的影响程度,其中主要研究分析了分别采用CCS舱段边界条件与LR舱段边界条件进行有限元计算而带来的结果上的差异。本文第二部分为港工建筑物中的大直径薄壁圆筒结构的有限元比较分析。在研究大圆筒的有限元比较分析中,主要讨论用不同的网格密度与数量来划分网格引起的差异。其中用了网格密,数量多与网格疏,数量少两种模型进行大圆筒结构的有限元分析对比

In the case the government and journalism undertook the equal rights and obligations and none wouldn't overtop the other.

新闻出版业的义务除了针对国家和政府以外,还出现了更多的针对公民权益和社会利益而限制新闻出版业的的新闻法规,同时,政府的义务也是针对新闻出版业、公民和社会而设。

The system description is concentrated on units' function. The principle of induction generator describes how to generate electric power from induction motor and the difference between synchronous generator and induction generator. The design of induction generation system is focus on a 80kW biogas induction generation and controlled parallel panel. Finally, the peripheral unit design is interesting in biogas storage tank, hot air duct and safety device .

系统描述针对感应式沼气发电系统所需设备的功能说明;感应发电机原理机针对马应马达与发电机之运转模式说明,并比较同步式与感应式发电机之差异;感应发电系统设计则针对80kW感应式沼气发电系统所需发电机组、保护并联盘设计说明;周边设施包括沼气储袋、引擎热风导管及沼气安全装置。

Secondly,for widespread IPO underpricing under Bookbuilding issuing method and abnormal high IPO underpricing in Chinese stock market,we propose policy recommendations at two different levels.The policy recommendation for Chinese stock market is to introduce short-selling mechanism,for widespread IPO underpricing under Bookbuilding issuing method is to open when-issued market of IPO shares to improve Bookbuilding issuing method.

然后,针对累计投标询价机制下的IPO抑价现象尤其是中国股市IPO抑价畸高的现象,从两个层面提出相关政策建议:针对中国股市的政策建议是引入卖空机制;针对全球股市普遍存在的IPO抑价现象,提出的政策建议是开放IPO股票市前交易来完善累计投标询价机制。

And an on-line adaptative training algorithm of the weights of neuron is given, which enhances fusion precision of the model, fault tolerance and adaptive ability for subsystems 4 In order to diagnose the soft fault of integrated system, a new fault detection method is proposed based on federated filter, which can detect fault of system in short interval and greatly enhance the method sensitivity to fault A fault diagnosis method of Dynamically Tuned Gyro baesd on parameter estimation of kinetics model of rotor, and signal pretreatment, parameter estimation and strategy of fault identification are studied in detail The method can locate fault of DTG correctly and effectively, which provides a new approach and idea for gyro fault diagonsis 5 In order to diagnose the hard fault of integrated system, fault tree analysis is studied on fault disgnosis of integrated system circuits, and the hardware and software of disgnosis system are developed, with faults located to main module and circuit For futhur diagnosis, an optimal measurement technology in detection of circuits is discussed by improving an algorithm based on information theory, and a pratical method based on half-split method is proposed.

针对组合系统的软故障诊断问题,提出了基于联合滤波模型的一种新的故障检测算法,给出了算法的理论推导过程,该算法能在较短的时间内检测出系统故障,提高了算法对故障的敏感度;研究了基于动调陀螺转子动力学模型参数辨识的故障诊断方法,就其中的信号预处理、参数辨识及故障决策方法等关键技术进行了详细的研究,该方法可以正确、有效地检测与定位动调陀螺故障,为陀螺故障诊断提供了新的途径和思路。 5。针对组合系统的硬故障诊断问题,研究了基于系统浅知识推理的故障树法在组合系统电路子系统故障诊断中的应用,开发了相关诊断系统的软硬件,故障可定位到系统的主要功能模块和电路板级。为了进一步的故障定位,研究了电路板故障的最优检测问题,完善了基于信息理论的优化算法,并提出了基于&对分法&的一种实用的优化算法,故障可定位到电路板的主要功能元件级。在组建的SINS/GPS组合系统实验平台上,针对上述研究内容,进行了大量的实验研究。

The majior contributions are summarized as follows:1. Enhanced the concept of min-max transfer capacity, which is the conservative transfer ability against the optimistic transfer ability that ATC evaluates.2. Constructed a TRM model of bi-level optimization based on leader-follower decision-making model, and an analytic method of computing TRM is proposed. A bi-section search algorithm simplifies and speeds up the calculation.3. Proposed the min-max transfer capability model considering steady voltage stability. The BiGA algorithm used in this model is shown to be robust.

本文针对这些问题,补充、提出了一些方法和见解,具体研究成果如下:1、针对ATC评估的是&乐观&情况下电网输电能力,提出了&保守&情况下的电网输电能力-至少传输容量的概念。2、提出基于主从决策的TRM双层优化模型,给出了TRM计算的解析方法,两次一维搜索的算法减小了TRM求解的难度,提高了计算速度。3、提出考虑静态电压稳定约束的至少传输容量评估模型,针对模型特点提出的BiGA算法具有较强的鲁棒性。

In building the simulation models for manufacturing missions, based on the combination with the multi-agent method and the GOOPN method, the compoundable method is presented, which makes the models more intelligent. Based on the result above, combined with the GOOPN models and the GERTS model, we put forward EPN-GERTS model through embedding Petri net into GERTS model, which can simplify the complexity in building models, and also improve the defects of GERTS model in describing resource conflicts and Petri net model in building models. In building the simulation models for development missions, the improved GERTS model is present to describe the development processes in QRMS. Through dynamically computing the redo times in GERTS, the improved model can reflect the operation character of development process. The simulation results are more precise when we use the models above.

针对构建快速研制系统的仿真对象问题,提出了基于多Agent的快速研制系统可靠性复合仿真建模方法,增强了仿真模型的智能性;在此基础上,针对制造任务,将Petri网嵌入到GERTS网中,提出了用于快速研制系统制造任务可靠性仿真的EPN-GERTS模型,简化了建模过程,同时也能考虑任务之间的资源冲突;针对研制任务,提出了用于快速研制系统研制任务可靠性仿真的改进GERTS模型,该模型可以描述设计迭代过程的动态运行特点,使仿真结果更加准确。

Six experimental stages were designed in our procedure, those are:(1) metabolite recovery and tested sample preparation: the metabolites were recovered by Amberlite XAD-2 absorption, followed by MeOH elution and solvent concentration;(2) antioxidant detection and strain selection: samples were quantitatively analyzed by the inhibition effects on formation of lipid peroxides and TBARS to screen the strains able to produce antioxidants. According to the established screening methods, we chose out a strain of actinomycetes, designed as AMBL-029C;(3) antioxidant purification: the fermentation broth was recovered by a series of separation techniques including centrifugation, Amberlite XAD-2 absorption, followed by MeOH elution and a successive TLC purification. The resulting primary purified compound [temperately designed as AMBL-029C-TS] was further analyzed by HPLC to monitor its purity;(4) physical-chemical characteristics: judging from the acid-base fractionation experiments, and the pH and temperature stability tests, the compound was deduced to be a acidic compound with the properties of low polarity and highly pH and temperature stable;(5) mechanism of the antioxidant: in comparison with some other known antioxidants, TS was subjected to investigate its antioxidant mechanism, together with BHT,-tocopherol, as well as two streptomyces metabolites, homogentisic acid and -phenylpyruvic acid, which were previously isolated as the natural antioxidants in our laboratory.

针对本实验目的,我们设计了以下的实验步骤﹔(1)二次代谢物回收及检测样本处理:我们将发酵所得的培养上清液,利用疏水吸附性树酯Amberlite XAD-2吸附回收,并以甲醇溶离及真空减压浓缩脱水等方式处理,以取得提供抗氧化活性筛选之检测样本;(2)抗氧化活性检测及菌种筛选:以「过氧化脂质」和「硫丙二醯尿」的生成量进行定性定量分析以作为抗氧化物质生产菌筛选之用;经此筛选程序,我们选获了具有抗氧化物质高生产力的菌株,命名为AMBL-029C;(3)抗氧化物质的分离纯化:针对生产菌株的发酵回收处理液,以矽胶薄层色层分离法经物质层析纯化后,并以高效能液相层析法(High performance liquid chromatography;HPLC)分析物质可得一初级纯化物质,命名为AMBL-029C-TS;(4)抗氧化物质的物理化学性质分析:由酸碱转溶(acid-base fractionation)实验得知,此抗氧化物质属於中低极性的强酸性物质,对温度(37℃-100℃)及酸碱度(pH3.0-13.0)均表现出高稳定性;(5)在抗氧化机制探讨方面,我们针对数种不同的抗氧化机制进行探讨,即: 1。

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If you are unfortunate enough to the lovelorn, please tell me, I will help you out, really, please contact me!

如果你不幸失恋了,请告诉我,我会帮助你摆脱困境,真的,请联系我啦!

China's plan to cut energy intensity by 20 percent and pollutant discharges by 10 percent between 2006 and 2010 is a case in point.

中国计划在2006年到2010间降低20%的能源强度和减少10%的主要污染物排放,就是一个这样的例子。

Well, Jerry would rattle off all the details of that movie.

那么,杰瑞会急促背诵那部电影所有细节。