观察

Objective To examine the value of Edaravone in the treatment of vascular dementia through ethologic and pathologic changes induced by chronic cerebral ischemiaMethods Wistar rats were randomly divided into sham operated group,model group,edaravone group and duxil groupThe chronic cerebral hypoperfusion models were made by permanent bilateral occlusion of both common carotid arteriesThe memory ability and ultramicrostructure of brain tissue were observedResults The escape latencies in model group were more significantly prolonged than that in the other groups (P＜005)But there was no significant difference between edaravone and duxil groupAnd pathologic changes observed by electron microscope in nerve cell of edaravone group and duxil group and model group were fairly light than that in sham operated groupEdaravone group had increased mitochondrium and frontal membranous synaptic vesicle and more productive metabolic activityConclusions Edaravone can lessen injures of hippocampal neurons loss to improve the spatial learning and memory abilitiesAnd it is proved further that edaravone can treat VD

目的 观察依达拉奉对慢性缺血致血管性痴呆大鼠行为学及形态学的影响，探讨依达拉奉在治疗VD中的应用价值。方法实验分假手术组、模型组及都可喜对照组及依达拉奉治疗组，应用双侧颈总动脉结扎方法制备慢性脑缺血大鼠痴呆模型，观察各组大鼠的记忆功能及脑组织超微结构改变。结果缺血组水迷宫表现同对照组、都可喜组及依达拉奉组相比有显著差异(P＜005)，依达拉奉组与都可喜组相比未见显著差异。同时电镜观察结果也发现依达拉奉组及都可喜组与模型组及假手术组相比神经细胞病理改变较轻，且依达拉奉组线粒体及前膜突触小泡增多，代谢活动较都可喜组旺盛。结论依达拉奉可能通过减轻海马神经元的损伤来改善慢性脑缺血大鼠空间学习记忆障碍，证明依达拉奉对慢性缺血致VD大鼠具有治疗作用。

The hypercholesterolemic rats were selected to imitate early stage of human atherosclerosis The effects and mechanism of n-3 PUFA on endothelium were investigated with the following parameters: the morphological appearance and arrangement of endothelium stained by Ag, in vitro endothelium-dependentrelaxation of aortic ring, endothelial permeability test using Even′s Blue, blood lipid and body weight in one-week intervals, excitative and emissive spectrum of RBC membrane labeled by DPH (1, 6-Diphenyl-1, 3, 5-hexatriene), fluidity of RBC membrane, plasma and RBC membrane LPO

在整体和离体水平，选用大鼠高脂血症模型模拟动脉粥样硬化早期状况。用114只大鼠多指标着重研究n-3多不饱和脂肪酸在高脂血症状态下对内皮的保护作用，以探讨其预防动脉粥样硬化的机理。所采用指标包括：浸银法观察内皮细胞排列与形态；离体血管环观察内皮依赖性舒张；伊文氏蓝染料法观察内皮通透性。为探讨作用机理，进行了血脂和体重的动态监测、DPH(1，6-二苯基-1，3，5，乙三烯)标记红细胞膜的荧光光谱测定、红细胞膜流动性测定、血浆和红细胞膜脂质过氧化物测定。

Following studies had been performed:(1)repetitive WLT and real-time ultrasonography had been performed to 18 HS and 40 FD toassess the reproducibility and influencing factors.(2) correlations of WLT with symptomscore and electrogastrography had been analysed.(3) differences ofWLT between HS and FD had been compared.(4) methophenolane andimmunohistochemistry stain of gastric fundus mucosa biopsy from 40 FD patients hadbeen performed to analyse the correlations of symptom score and WLT with count andactivity of mast cells, 5-HT level, and H.Pylori infectious status.(5) the effect of oralsumatriptan to proximal stomach function in 10 HS and 10 FD had been assessed, so as toestablish the possible regulating mechanism of fundic relaxation, explore the therapeuticpotential and feasibility of sumatriptan on FD.Results:I. Evaluation of WLT and its application in FD management.

我们主要进行以下几个方面的研究：(1)对18名健康人及40名FD患者重复进行水负荷试验并结合B超声对近端胃的实时监测，评价水负荷试验结果的可靠性并分析其影响因素；(2)观察水负荷试验结果与症状积分及体表胃电结果的相关性；(3)比较FD患者和健康成人水负荷试验结果的差别；(4)对40名FD患者胃底粘膜活检组织进行免疫组化染色和甲苯胺蓝染色，观察FD患者的症状及水负荷试验结果与胃底粘膜肥大细胞、5-HT表达情况及H.pylori感染情况的相关性：(5)观察口服舒马曲坦对10名健康人及10名FD患者近端胃功能的影响，并由此推测胃底舒张运动调节的生理机制，探讨口服舒马曲坦治疗FD的可行性。

Methods The antipyretic activity of SMPS was observed in rats with high temperature induced by dried yeast and 2,4-dinitrophenol; biliary duct cannula bile at 0.5,1,1.5,2,2.5 h after intraduodenal administration in rats was collected and counted to observe the effect of SMPS on biliary secretion;the weight of cholecyst in mice was counted to observe the effect of SMPS on the cholecyst of mice;the acute toxicity test of SMPS was done with mice.

采用干酵母、2，4二硝基苯酚致大鼠发热的模型观察玉米须多糖的解热作用；采用胆管引流法分别收集并计量给药后0.5，1，1.5，2 ，2.5 h的胆汁的方法观察SMPS对肝脏分泌胆汁的影响；采用测定小鼠胆囊重量的方法观察SMPS对胆囊的影响；对SMPS进行了小鼠急性毒性实验。

MethodsThe antipyretic activity of SMPS was observed in rats with high temperature induced by dried yeast and 2,4-dinitrophenol; biliary duct cannula bile at 0.5,1,1.5,2,2.5 h after intraduodenal administration in rats was collected and counted to observe the effect of SMPS on biliary secretion;the weight of cholecyst in mice was counted to observe the effect of SMPS on the cholecyst of mice;the acute toxicity test of SMPS was done with mice.

目的研究玉米须多糖的清热利胆作用及急性毒性。方法采用干酵母、2，4二硝基苯酚致大鼠发热的模型观察玉米须多糖的解热作用；采用胆管引流法分别收集并计量给药后0.5，1，1.5，2 ，2.5 h的胆汁的方法观察SMPS对肝脏分泌胆汁的影响；采用测定小鼠胆囊重量的方法观察SMPS对胆囊的影响；对SMPS进行了小鼠急性毒性实验。

MethodsThe antipyretic activity of SMPS was observed in rats with high temperature induced by dried yeast and 2,4-dinitrophenol; biliary duct cannula bile at 0.5,1,1.5,2,2.5 h after intraduodenal administration in rats was collected and counted to observe the effect of SMPS on biliary secretion;the weight of cholecyst in mice was counted to observe the effect of SMPS on the cholecyst of mice;the acute toxicity test of SMPS was done with mice.

方法采用干酵母、2，4二硝基苯酚致大鼠发热的模型观察玉米须多糖的解热作用；采用胆管引流法分别收集并计量给药后0.5，1，1.5，2 ，2.5 h的胆汁的方法观察SMPS对肝脏分泌胆汁的影响；采用测定小鼠胆囊重量的方法观察SMPS对胆囊的影响；对SMPS进行了小鼠急性毒性实验。

We introduced improved primary mixed glial culture and different-attachment method to isolate and purify the OPCs, the cells were proliferated in serum-free medium, flow cytometry and immunohischemistry methods were employed to estimate the purity of cultured OPCs. Their abilities of differentiation and expression of trophic factors were identified by RT-PCR and immunostaining. Several methods including TUNEL and MTT were adopted to estimate the protective effects of conditioned culture medium from oligodendrocyte lineage cells on the primary cultured cerebellar granule neurons. Intravitreal transplant of OPCs, combined with retrograde fluorescent labeling the superior colliculus and intraorbital optic nerve transection, were used to investigate the protective effects of OPCs on the axotomized RGCs in vivo. Intravitreal transplantof OPCs or NSCs on the newborn rats, and retinal transplant of OPCs on the young rats were performed, to observe the myelin formation in the retina at different stages after cellular transplantation. Optic nerve transection was carried out on some rats with myelinated retinae, to study the influence of myelination on the injuried RGCs.

为此，本研究采用改良的胶质细胞混合培养与差速贴壁方法获得大鼠OPCs，使用无血清培养基进行扩增、培养，用免疫组织化学和流式细胞技术对培养细胞的纯度进行鉴定，对少突胶质系细胞表达部分营养因子的情况进行检测；采用TUNEL、MTT等方法对少突胶质系细胞条件培养基对原代培养小脑颗粒神经元的保护作用进行检测；将OPCs移植入成年SD大鼠玻璃体内，利用上丘逆行荧光标记技术，观察眼内移植的OPCs对眶内视神经切断时的视网膜神经节细胞的保护作用及其持续时间；将OPCs或NSCs移植入新生和幼年SD大鼠玻璃体或视网膜内，观察不同时期视网膜内髓鞘形成与分布特点，分析髓鞘的超微结构，并观察眼内髓鞘形成对损伤神经节细胞的保护作用。

Activities in the activity zone was an important component of the kindergarten children's day activity. The kindergartener's observation on the activity zone was the premises to practice effective guidance. The proper way and view angle should be chosen.

活动区活动是幼儿一日活动的重要组成部分，教师对整个活动区的观察是实施有效指导的前提：要选择适宜的观察方式和恰当的观察角度。

An acute observer of politics and politicians; incisive comments; icy knifelike reasoning; as sharp and incisive as the stroke of a fang; penetrating insight; frequent penetrative observations.

对政治和政治家的敏锐观察者；尖锐的评论；冷静敏锐的推理；象犬牙的撞击一样锋利和尖锐；明察秋毫的观察；频繁敏锐的观察。

An acute observer of politics and politicians; incisive comments; icy knifelike reasoning; as sharp and incisive as the stroke of a fang; penetrating insight; frequent penetrative observations.

对政治和政治家的敏锐观察者；尖锐的评论；冷静敏锐的推理；象犬牙的撞击一样锋利和尖锐；明察秋毫的观察；频繁敏锐的观察。精辟的分析。

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克，果实色泽鲜艳，汁多味浓，甜酸适口，离核仁甜，清末时就驰名陕、甘两省，清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异：红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感，ED90值分别为47.65、71.67和29.17g/hm2；春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感，ED90值分别为96.91、114.20g/hm2；而番茄不敏感。