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Blood samples were collected before and after extracorporeal circulation to assess platelet adhesiveness, aggregation, platelet count.

测定两组转流前、后血小板粘附率、血小板聚集率、血小板计数,观察术后24小时出血量和输血量。

Fluorescein isothiocyanate labeled polyclonal goat antihuman immunoglobulin antibody was added, and flow cytometer was used to detect bead-platelet-associated autoantibodies-antihuman immunoglobulin antibody complex. Results The fluorescene ratio of four monoclonal antibodies was significantly different (P .01) between the idiopathic thrombocytopenic purpura patients and either the non-ITP patients or the normal controls. If the upper limit of normal control was set as cutoff value, ratios of greater than 1.37, 1.24, 1.48, and 1.19 were considered positive for the four monoclonal antibodies respectively. The flow cytometric bead assay had an overall sensitivity of 73.17% and a specificity of 94.29%.

结果 特发性血小板减少性紫癜组4种单抗荧光强度比值与非ITP血小板减少组和正常对照组有显著性差异(P<0.01);若将ITP组患者4种单抗荧光强度比值分别大于正常对照组上限1.37、1.24、1.48和1.19判断为阳性,则流式微球技术检测血小板特异性自身抗体的敏感性为73.17%,特异性为94.3%;4种单抗联合检测总体敏感性明显高于改良间接单抗特异的血小板抗原固定试验(P<0.05,且大于各单个抗体检测敏感性。

Objective To study the in vitro quality of apheresis platelets stored in platelet additive solutions.

目的:探讨血小板添加液保存单采血小板的效果,观察其对血小板功能的影响。

Transfusion of apheresis platelets can increase the number of platelets in peripheral blood obviously and achieve hemostasis quickly.

血小板减少的患者,输注机采血小板可明显提升外周血中的血小板数量,并能迅速起到止血的目的,防止大出血并发症。

The current model of apheresis platelets is safe,24-hour lock-platelet bacteria screening method in the prevention and control of bacterial contamination in platelets there is a certain shortcomings and deficiencies.

目前的单采血小板模式是安全的, 24 h锁定的血小板细菌筛检方法在预防和控制血小板细菌污染方面存在一定的缺点和不足。

Objective To investigate the current apheresis platelet bacterialcontamination and to explore the probability of a 24-hour lock-platelet bacteria screening method in the prevention and control of the effectiveness of plateletbacterial contamination.

目的 调查目前单采血小板细菌污染的概率及探讨24 h锁定的血小板细菌筛检方法在预防和控制血小板细菌污染的有效性。

The effects of 100% and 50% eluent of PLGA/RIP on the leucocyte, erythrocyte, thrombocyte and platelet aggregation were detected.

PLGA/RIP对兔白细胞、红细胞和血小板血小板聚集的影响实验:观察PLGA/RIP洗提液原液和0.5 g/L洗提液对兔白细胞、红细胞和血小板血小板聚集的影响。

Methods The 26th exon, the 30th exon and the 21st intron of gene GP Ⅱb in 110 individuals were amplified by polymorphism and sequenced to investigate whether there was linkage among the polymorphisms of the gene. Human platelet antigen-3 (HPA-3) gene frequency was detected by Fok 1 enzyme in 147 patients with hematologic diseases, and was compared with that in 110 normal individuals. Forty-four patients who received apheresis platelet transfusion repeatedly were randomly divided into the HPA-3 homotype group and the control group. The antibodies of the platelet were detected after 3 times of platelet transfusion.

聚合酶链反应-单链构型多态性分析检测110例正常人GP Ⅱb基因第26和30外显子(Exon 26, Exon 30)及21内含子(Intron21)基因多态性,进行基因序分析,研究这些基因多态性是否存在连锁关系;应用Fok1酶切法对147例血液病人人类血小板抗原-3(human platelet antigen-3, HPA-3)基因进行分型,并与110例正常人进行比较;将接受单采血小板输注的44例血液病人随机分为HPA-3同型输注组和对照组,血小板输注3次以后检则血小板抗体。

It is concluded that raising content of oxygen in platelet plasma can provide more oxygen to compensate oxygen supply deficiency for platelet metabolism and improve the efficiency of platelet oxygenic metabolism and the quality of platelet during preservation.

提高血小板血浆中的含氧量可弥补保存袋中血小板代谢的供氧不足,提高血小板的有氧代谢和血小板的保存质量。

The number of platelet antibody crossmatch coincidence in 112 specimens of AA, 42 specimens of MDS and 95 specimens of leukemia were 45, 20 and 40, the coincidence rates were 40.18%、47.62% and 42.11%.The mean corrected count increment in 20 patients received platelet transfusion many times was 18.2 after crossmatch and 4.7 before crossmatch.

多次输注血小板的112个AA血液标本、42个MDS血液标本和95个白血病血液标本的血小板抗体交叉相合数分别为45、20和40个,相合率分别为40.18%、47.62%和42.11%; 20例多次输注血小板的病人交叉配合前的血小板增高指数平均为4.7,交叉配合后为18.2。

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