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花粉管

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The results showed that: different concentrations of sucrose and boric acid had significant impact on pollen germination, but different concentrations of calcium had no significant impact on pollen germination. It was most appropriate for tube elongation when sugar 150 gL^(-1) and boric acid 100 gL^(-1). Lack of sucrose would lead to the accumulation of callose in top of pollen tube and hold back its growth. The distribution of callose was regular in pollen tube when the medium lacked boric.

结果表明:蔗糖、硼酸浓度间对花粉萌发有显著影响,钙浓度间对花粉萌发的促进作用不明显;蔗糖150 gL^(-1)、硼酸100 mgL^(-1)时最适宜花粉管伸长;蔗糖的缺乏会导致胼胝质在花粉管顶端积累,使其生长停滞,缺硼时胼胝质在花粉管内分布正常。

When pollen germinates on a stigma the growing pollen tubes deposit callose as small plugs, but if the plant is self-incompatible, tubes from its own pollen will deposit more callose, which then blocks the tube, preventing fertilization.

当花粉在柱头上萌发时,生长的花粉管产生少量胼胝质,但如果该植物是自交不亲和的,那么花粉管将产生比较多的胼胝质,可以阻塞花粉管来阻止受精。

Pollen grains germinated soon after pollination. Pollen tubes grew first into the intercellular space of branched penniform stigma and then through the intercellular space of transmitting tissue into the top of the ovary, further penetrating the ovary wall and primine to the micropyle. Green fluorescence was found in both pollen and pollen tubes.

结果表明:授粉后,花粉随即萌发,花粉管进人羽毛状柱头分支结构的细胞间隙,继续生长于花柱至子房顶部的引导组织的细胞间隙中,而后进入子房,在子房壁与外珠被之间的缝隙中向珠孔方向生长,花粉与花粉管均具有明显的绿色荧光。

The effect of IAA on pollen germination and pollen tube growth was in significant.

结论]珙桐花粉萌发和花粉管的生长因培养基组分和培养条件的的改变而不同,其花粉萌发和花粉管生长不仅需要一个适宜的组分浓度,也需要有一个合适的培养条件。

It takes about 0.5 h for a pollen tube from germination to arrive at the micropyle. About 0.5 h after pollination, the pollen tube releases two sperms into one synergid. At 0.5-2.5 h after pollination, the egg cell fuses with one sperm to form a zygote. About 10.0 h after pollination, the division of the zygote begins. The duration of the zygote lasts about 2.5-10.0 h. However, one sperm nucleus fuses with two polar nuclei at 1.0-3.0 h after pollination, and 5.0 h after pollination the primary endosperm nucleus begins to divide.

水稻受精过程经历的时间表如下:授粉后,花粉在柱头萌发;花粉萌发至花粉管进入珠孔大约需要0.5小时;授粉后0.5小时左右,花粉管进入一个助细胞,释放精子;授粉后0.5-2.5小时,精卵融合形成合子;授粉后约10.0小时,合子第1次分裂,合子期为授粉后2.5-10.0小时;授粉后1.0-3.0小时,精核与两极核融合;授粉后约5.0小时,初生胚乳核分裂。

Pollen grains germinated soon after pollination. Pollen tubes grew first into the intercellular space of branched penniform stigma and then through the intercellular space of transmitting tissue into the top of the ovary, further penetrating the ovary wall and primine to the micropyle. Green fluorescence was found in both pollen and pollen tubes.

结果表明:授粉后,花粉随即萌发,花粉管进入羽毛状柱头分支结构的细胞间隙,继续生长于花柱至子房顶部的引导组织的细胞间隙中,而后进入子房,在子房壁与外珠被之间的缝隙中向珠孔方向生长,花粉与花粉管均具有明显的绿色荧光。

Specific recognition and degradation of stylar S-RNase for self-pollen tube RNA, resulted in the stop of self-tube growth in Prunus mume. The recognition specificity of stylar S-RNase for the RNA weakened with the rise of temperature, consequently regulating the self-incompatibility reaction in Prunus mume.

果梅花柱S-RNase特异性识别并降解自花花粉管RNA,导致自花花粉管停止生长;随着温度的升高花柱S-RNase对花粉管RNA的识别特异性减弱,从而调节果梅的自交不亲和性反应。

With the aid of aniline blue staining, pollen tube growth in pistil tissue was observed in Arabidopsis thaliana, and the growth speed of pollen tubes in style and ovary were calculated, the results showed that the pollen tube grew faster as time goes on.

以模式植物拟南芥为供试材料,利用脱色苯胺蓝染色的方法观察了拟南芥花粉管在雌蕊组织中的生长情况,获得了花粉管从萌发到进入花柱和子房不同时期的生长速率,结果表明,花粉管在不同时期的生长速率呈现出逐步加快的趋势。

In this study, in order to examine the dynamics of tip [Ca2+]i together with the dynamics of tip-localized F-actin in vivo, a kind of double labeled material was constructed. The Ca2+ and actin microfilaments of Arabidopsis pollen tubes were labeled by cameleon and GFP-mTalin respectively.

本研究以拟南芥花粉管为材料,通过转基因技术,将分别标记Ca~(2+)和微丝的两种荧光蛋白cameleon与GFP-mTalin在花粉管中同时表达,实现活体花粉管中Ca~(2+)与微丝的同时标记。

The results showed that pollen in differentfamily or genus of plant have different requirements for phytohormones.The basicmedium of pollen culture supplemented with 2ppm NAA and GA_3 can promotes thepollen germination in Cruciferae.It has been proved that basic medium supplemtedwith 2ppm IAA and GA_3 has special effect on pollen germination and elongation ofpollen tube of woody plant in Rosace...

结果证明:不同科属的植物花粉对激素的要求不同,如在花粉萌发的基本培养基上加2ppm的NAA和GA_3混合液对十字花科植物花粉有促进作用,加2ppm的IAA和GAa_3混合液对蔷薇科木本植物花粉萌发和花粉管伸长有显著效果,而葫芦科植物的花粉在基本培养基上只要有无机盐离子就可以萌发,激素仍有促进花粉萌发的作用。

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