花粉管

Tumefaciens mediated transformation using cotyledonary node as explants was optimized; 3 methods including cutting chapiter, ovary injection and chapiter spreading for pollen tube pathway was compared. Cutting chapiter and ovary injection can both achieve insect resistant gene tranformants.

优化了以子叶节为外植体的农杆菌介导转化技术；比较了切割柱头、子房注射和子房涂抹3种花粉管通道法处理授粉后黄瓜子房的转化效果，切割柱头和子房注射均可成功获得黄瓜抗虫转基因植株。

Effects of ion beam implantation on pollen germination and pollen tube growth of Cedrus deodara were investigated by the laser confocal microscopy technique.

用激光扫描共聚焦显微技术研究了低能离子注入对雪松花粉管萌发和生长的影响。

Wheat and durum were taken as acceptors and the DNAs of 7 different plant materials were taken as donors, authors had studied seed-bearing ratio of modern generation plants transformed by pollen tube pathway. The results were shown as follow.

以普通小麦和硬粒小麦为受体、以亲缘关系不同的7个材料作供体，研究了花粉管通道法转化植株的当代结实率。

In the present review, we focus on some of the recent advances and some key developmental events revealed in the fertilization process, including the role of the egg apparatus in guiding pollen tube orientation, sperm cell movement to their targeting cells, interaction between gametes, egg cell activation, and onset of embryogenesis and endosperm development.

本文着重讨论受精作用的若干重要发育事件，包括受精前卵器细胞对花粉管向胚珠定向生长的近距离引导信号，精子的靶向运动，精、卵细胞相互作用和配子融合后卵细胞的激活与中央细胞发育的启动等。

In second stage,after 2-nucleate embryo sac development,most pollen tubes grow radially around ovules.

在亲和受精过程中，花粉管的生长速度和方向性已成为人们普遍关注的焦点。

With rye cultivar AR71 as the source of resistance to wheat sharp eyespot,we tried to transfer the rye resistant genes into eight wheat cultivars or lines by pollen tube pathwaymethod and intergeneric crossing between wheat and rye.

以抗小麦纹枯病的黑麦AR71为抗源供体，以鲁麦1号、有蜡、百农3217、圆锥小麦TG29等8个小麦品种为受体，试图通过花粉管通道法和属间杂交的方法，导入供体的抗纹枯病基因。

Were done and no positive reaction was found.5. The DNA of the young leaves of 132 Bt transgenic plants was extracted to do PCR Five positive inversion plants were obtained and the inversion rate was 3.8%.6. The DNA of fire positive inversion plants obtained in Dl generation was extracted and tested. Two stable positive inversion plants were obtained in D2 generations.7 The way of flourescent mirror flat was used to observe the pollen tube extension circumstance in the flower post after self-pollination.

检测了132株，没有发现阳性反应。5、对转入Bt基因的132株植株的幼叶提DNA，作PCR检测。D1代获得5株阳性转化植株，分别是90035的4株，85—593的1株，转化率为3.8％。6、将D1代获得5株阳性转化植株的种子在温箱中发芽，提取DNA检测，得到D2代稳定遗传的阳性转化植株2株。7、应用荧光镜制片法观察大豆自花授粉后花粉管在花柱中延伸情况。

The purpose of this study was to establish an efficient and stable gene transfer system for Phalaenopsis via pollen tube pathway. For this purpose, the growth and development of ovary and megaspore mother cell in Phalaenopsis was microscopically examined.

本研究主要是以花粉管法进行蝴蝶兰之基因转殖，希望建立效率高且稳定的转殖系统，以应用於蝴蝶兰育种。

Pollens germinated readily 3 days after pollination and megaspore mother cells underwent meiotic division at about 50 days after pollination.

本试验主要分为两部分；花粉管生长及受精之观察：蝴蝶兰之子房和大孢子母细胞，於授粉后才开始发育。

In third stage,at maturing of the embryo sac,pollen tubes grow directionally toward micropyle into embryo sac.

然而由于技术上存在困难，有关花粉管活体状态下生长的研究很少[1]。

He and Nina moved to California and lived at 2005 Ivar Street, Apt.

他和Nina搬到加州，并在2005年伊瓦尔街，公寓生活。

Droperidol （ potently inhibits transfected HERGchannels and this is the probable mechanism for QT prolongation.

氟哌利多有效地抑制了转染的 HERG 钾通道，可能是 QT 间期延长的机制。